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PMID: 2438428 Published · ppublish English Comparative Study Journal Article Research Support, Non-U.S. Gov't

Isolation of novel human retrovirus-related sequences by hybridization to synthetic oligonucleotides complementary to the tRNA(Pro) primer-binding site.

Journal of virology ·Vol. 61 ·No. 7 ·1987-07-00 ·Pages 2071-5

Kröger B, Horak I

Abstract

Synthetic oligonucleotides complementary to putative retroviral primer-binding sites were used as hybridization probes to detect novel retroviruslike sequences. An 8.1-kilobase element with structural features of a retroviral provirus was isolated from a human genomic library by this approach. Nucleotide sequence analysis of its 600-base-pair long terminal repeats revealed characteristic motifs known as regulatory signals for RNA polymerase II transcription: CCAAT, TATA, and ATTAAA. In addition, a putative pol gene displays apparent homologies to conserved regions of retroviral reverse transcriptase. The 5' long terminal repeat is flanked at its 3' end by a putative primer-binding site for reverse transcription with homology to tRNA(Pro). This element is therefore termed HuRRS-P (human retrovirus-related sequence-proline). There are 20 to 40 copies of HuRRS-P homologous sequences in DNAs of human and simian origin.

MeSH Terms
Animals Binding Sites DNA/genetics DNA Transposable Elements DNA, Recombinant/isolation & purification Humans Oligodeoxyribonucleotides/chemical synthesis,genetics Phylogeny Primates/genetics RNA/genetics RNA Polymerase II/metabolism RNA, Transfer/genetics RNA, Viral/genetics RNA-Directed DNA Polymerase/genetics Repetitive Sequences, Nucleic Acid Retroviridae/genetics Sequence Homology, Nucleic Acid Viral Proteins/genetics
Chemicals
DNA Transposable Elements DNA, Recombinant Oligodeoxyribonucleotides RNA primers RNA, Viral Viral Proteins RNA DNA RNA, Transfer RNA Polymerase II RNA-Directed DNA Polymerase
Authors & Affiliations
2 authors, click to expand affiliations / ORCID
Kröger B
Horak I
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28 references, click to expand
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Article Info
Journal
Journal of virology
Abbr.
J Virol
ISSN
0022-538X
DOI
10.1128/JVI.61.7.2071-2075.1987
Published
1987-07-00
Pages
2071-5
Language
English
Region
United States
NLM ID
0113724
PMCID
PMC254226
Subset
IM
Databases
GENBANK
M16779, M16780
External Links
PubMed source DOI: 10.1128/JVI.61.7.2071-2075.1987 PMC Full Text View journal details
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