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PMID: 2439066 Published · ppublish English Journal Article Research Support, Non-U.S. Gov't Research Support, U.S. Gov't, P.H.S.

Identification of a phosphoprotein in the nuclear matrix by monoclonal antibodies.

The Biochemical journal ·Vol. 241 ·No. 3 ·1987-02-01 ·Pages 693-7

Halikowski MJ, Liew CC

Abstract

Previous work in this laboratory has established that a rat liver nuclear phosphoprotein (B2:Mr 68,000, pI 6.5-8.2) is associated with actively transcribed nucleosomes, as demonstrated by its preferential release after mild treatment with micrococcal nuclease. In the present report we provide further immunological evidence ('Western Blot' analysis, solid-phase radioimmunoassay and indirect immunofluorescence) that in addition establishes the presence of this phosphoprotein in the nuclear-matrix protein fraction. This paradoxical localization suggests that this phosphoprotein may function in two separate and distinct roles within the realm of nuclear organization.

MeSH Terms
Animals Antibodies, Monoclonal Chromosomal Proteins, Non-Histone/immunology,isolation & purification Epitopes/analysis Fluorescent Antibody Technique Immunoelectrophoresis Liver/analysis Male Phosphoproteins/immunology,isolation & purification Protein Binding Radioimmunoassay Rats Rats, Inbred Strains
Chemicals
Antibodies, Monoclonal Chromosomal Proteins, Non-Histone Epitopes Phosphoproteins nuclear phosphoprotein B2, rat
Authors & Affiliations
2 authors, click to expand affiliations / ORCID
Halikowski M J
Liew C C
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31 references, click to expand
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Article Info
Journal
The Biochemical journal
Abbr.
Biochem J
ISSN
0264-6021
DOI
10.1042/bj2410693
Published
1987-02-01
Pages
693-7
Language
English
Region
England
NLM ID
2984726R
PMCID
PMC1147619
Subset
IM
External Links
PubMed source DOI: 10.1042/bj2410693 PMC Full Text View journal details
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