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PMID: 2445730 Published · ppublish English Journal Article Research Support, U.S. Gov't, P.H.S.

Streptococcus pyogenes type 12 M protein gene regulation by upstream sequences.

Journal of bacteriology ·Vol. 169 ·No. 12 ·1987-12-00 ·Pages 5633-40

Robbins JC, Spanier JG, Jones SJ, Simpson WJ, Cleary PP

Abstract

A partial nucleotide sequence that included 1,693 base pairs of the M12 (emm12) gene of group A streptococci (strain CS24) and adjacent upstream DNA was determined. Type 12 M protein-specific mRNA of strain CS24 is transcribed from two promoters (P1 and P3) separated by 30 bases. The transcription start sites of the emm12 gene were located more than 400 bases downstream of a deletion that causes decreased M-protein gene transcription in strain CS64. Deletion analysis of M protein-expressing plasmids indicated that an upstream region greater than 1 kilobase is required for M-protein gene expression. The M-protein gene transcriptional unit appears to be monocistronic. Analysis of the emm12 DNA sequence revealed three major repeat regions. Two copies of each repeat, A and B, existed within the variable 5' end of the gene; repeat C demarcated the 5' end of the constant region shared by emm12 and emm6.

MeSH Terms
Antigens, Bacterial/genetics Bacterial Outer Membrane Proteins Bacterial Proteins/genetics Base Sequence Carrier Proteins Cloning, Molecular DNA Restriction Enzymes DNA, Bacterial/genetics Gene Expression Regulation Genes, Bacterial Molecular Sequence Data Plasmids Promoter Regions, Genetic RNA, Bacterial/genetics Regulatory Sequences, Nucleic Acid Streptococcus pyogenes/genetics Transcription, Genetic
Chemicals
Antigens, Bacterial Bacterial Outer Membrane Proteins Bacterial Proteins Carrier Proteins DNA, Bacterial RNA, Bacterial streptococcal M protein DNA Restriction Enzymes
Authors & Affiliations
5 authors, click to expand affiliations / ORCID
Robbins J C
Department of Microbiology, University of Minnesota, Minneapolis 55455.
Spanier J G
Jones S J
Simpson W J
Cleary P P
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Article Info
Journal
Journal of bacteriology
Abbr.
J Bacteriol
ISSN
0021-9193
DOI
10.1128/jb.169.12.5633-5640.1987
Published
1987-12-00
Pages
5633-40
Language
English
Region
United States
NLM ID
2985120R
PMCID
PMC214017
Subset
IM
Grants
NHLBI NIH HHS · 5T32HL107114 · United States
NIAID NIH HHS · AI16722 · United States
Databases
GENBANK
M18269
External Links
PubMed source DOI: 10.1128/jb.169.12.5633-5640.1987 PMC Full Text View journal details
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