Home LiteratureArticle Details
PMID: 4928818 Published · ppublish English Journal Article

Purification and physical properties of group C streptococcal phage-associated lysin.

The Journal of experimental medicine ·Vol. 133 ·No. 5 ·1971-05-01 ·Pages 1105-17

Fischetti VA, Gotschlich EC, Bernheimer AW

Abstract

A purification procedure for the Group C phage-associated lysin is described utilizing tetrathionate to protect the enzyme's -SH group(s) from thiol-inactivating agents. A 652-fold purification has been accomplished yielding a solution in which the enzyme activity corresponds to essentially a single band on polyacrylamide gel which accounts for 70% of the total protein in the preparation. A molecular weight of 101,000 and frictional ratio of 1.526 was determined for the lysin utilizing experimentally determined values for its Stokes radius and sedimentation coefficient.

MeSH Terms
Acrylates Bacterial Proteins/analysis Bacteriological Techniques Bacteriophages Chromatography Densitometry Electrophoresis Enzyme Activation Gels Lysine/analysis,isolation & purification Lysogeny Molecular Weight Spectrophotometry Streptococcus/enzymology Thiosulfates/pharmacology
Chemicals
Acrylates Bacterial Proteins Gels Thiosulfates Lysine
Authors & Affiliations
3 authors, click to expand affiliations / ORCID
Fischetti V A
Gotschlich E C
Bernheimer A W
References (30)
30 references, click to expand
  1. Biosynthesis of streptococcal cell walls: A rhamnose polysaccharide.
    Science. 1963 Apr 26;140(3565):400-1 PMID: 14003420
  2. A method for determining the sedimentation behavior of enzymes: application to protein mixtures.
    J Biol Chem. 1961 May;236:1372-9 PMID: 13767412
  3. OBSERVATIONS ON THE GROUP C STREPTOCOCCAL BACTERIOPHAGE AND LYTIC ENZYME SYSTEM.
    J Bacteriol. 1965 Feb;89:496-502 PMID: 14255719
  4. Enzymic Activity of Bacteriophage-Culture Lysates: I. A Capsule Lysin Active against Klebsiella pneumoniae Type A.
    J Bacteriol. 1948 Nov;56(5):683-93 PMID: 16561619
  5. Studies on the bacteriophages of hemolytic streptococci. II. Antigens released from the streptococcal cell wall by a phage-associated lysin.
    J Exp Med. 1958 Dec 1;108(6):803-21 PMID: 13598814
  6. MOLECULAR EXCLUSION AND RESTRICTED DIFFUSION PROCESSES IN MOLECULAR-SIEVE CHROMATOGRAPHY.
    Biochemistry. 1964 May;3:723-30 PMID: 14193644
  7. INTRACELLULAR M PROTEIN OF GROUP A STREPTOCOCCUS.
    J Bacteriol. 1963 Mar;85:536-40 PMID: 14042930
  8. An immunological relationship between the group. A streptococcus and mammalian muscle.
    J Exp Med. 1966 Oct 1;124(4):661-78 PMID: 5922288
  9. Studies on L forms and protoplasts of group A streptococci. II. Chemical and immunological properties of the cell membrane.
    J Exp Med. 1963 Mar 1;117:377-99 PMID: 13959531
  10. DISC ELECTROPHORESIS. II. METHOD AND APPLICATION TO HUMAN SERUM PROTEINS.
    Ann N Y Acad Sci. 1964 Dec 28;121:404-27 PMID: 14240539
  11. Enzymatic properties of a phage-induced lysin affecting group A streptococci.
    J Bacteriol. 1962 May;83:1058-68 PMID: 13887529
  12. The active agent in nascent phage lysis of streptococci.
    J Gen Microbiol. 1957 Jun;16(3):584-95 PMID: 13439143
  13. Estimation of the molecular weights of proteins by Sephadex gel-filtration.
    Biochem J. 1964 May;91(2):222-33 PMID: 4158310
  14. Demonstration of the presence of a histidine residue at the active site of streptococcal proteinase.
    J Biol Chem. 1967 Sep 25;242(18):4029-32 PMID: 6061695
  15. Studies on the chemical structure of the streptococcal cell wall. I. The identification of a mucopeptide in the cell walls of groups A and A-variant streptococci.
    J Exp Med. 1961 Jul 1;114:127-40 PMID: 13754097
  16. Determination of molecular weights and frictional ratios of proteins in impure systems by use of gel filtration and density gradient centrifugation. Application to crude preparations of sulfite and hydroxylamine reductases.
    Biochim Biophys Acta. 1966 Feb 7;112(2):346-62 PMID: 5329026
  17. Preparation and antigenicity of M protein released from group A, type 1 streptococcal cell walls by phage-associated lysin.
    J Exp Med. 1960 Jul 1;112:77-96 PMID: 13751222
  18. FINAL PROOF FOR THE IDENTITY OF ENZYMIC SPECIFICITIES OF EGG-WHITE LYSOZYME AND PHAGE T2 ENZYME.
    Biochim Biophys Acta. 1963 Oct 29;78:369-70 PMID: 14099647
  19. Staphylococcal virolysin, a phage-induced lysin; its differentiation from the autolysis of normal cells.
    J Gen Physiol. 1957 May 20;40(5):791-807 PMID: 13428989
  20. STRUCTURE OF STREPTOCOCCAL CELL WALLS. IV. PURIFICATION AND PROPERTIES OF STREPTOCOCCAL PHAGE MURALYSIN.
    J Biol Chem. 1964 Dec;239:4027-33 PMID: 14247642
  21. Synthesis of capsular polysaccharide (hyaluronic acid) by protoplastmembrane preparations of group A Streptococcus.
    J Biol Chem. 1962 Feb;237:273-9 PMID: 14470033
  22. Purification and properties of a bacteriophage-induced cell wall peptidase from Staphylococcus aureus.
    J Bacteriol. 1967 Mar;93(3):1089-95 PMID: 4225890
  23. A phage-associated enzyme of Bacillus megaterium which destroys the bacterial cell wall.
    Virology. 1957 Dec;4(3):563-81 PMID: 13507314
  24. Specific chemical modification of proteins.
    Annu Rev Biochem. 1970;39:101-30 PMID: 4920818
  25. Studies on ficin. I. Its isolation and characterization.
    Biochemistry. 1968 Jan;7(1):163-75 PMID: 5758541
  26. Studies on bacteriophages of hemolytic streptococci. I. Factors influencing the interaction of phage and susceptible host cell.
    J Exp Med. 1957 Sep 1;106(3):365-84 PMID: 13463248
  27. GROUP N STREPTOCOCCAL PHAGE LYSIN.
    J Gen Microbiol. 1963 Jul;32:29-32 PMID: 14045520
  28. The interaction of oxidized glutathione, cystamine monosulfoxide, and tetrathionate with the-SH groups of rabbit muscle D-glyceraldehyde 3-phosphate dehydrogenase.
    J Biol Chem. 1962 Apr;237:1356-62 PMID: 14486599
  29. Purification and properties of a thermophilic bacteriophage lytic enzyme.
    J Virol. 1967 Jun;1(3):617-25 PMID: 4990043
  30. Studies of L forms and protoplasts of group A streptococci. I. Isolation, growth, and bacteriologic characteristics.
    J Exp Med. 1959 Dec 1;110:853-74 PMID: 13824817
Article Info
Journal
The Journal of experimental medicine
Abbr.
J Exp Med
ISSN
0022-1007
DOI
10.1084/jem.133.5.1105
Published
1971-05-01
Pages
1105-17
Language
English
Region
United States
NLM ID
2985109R
PMCID
PMC2138921
Subset
IM
External Links
PubMed source DOI: 10.1084/jem.133.5.1105 PMC Full Text View journal details
Analysis Services
Analysis Services

Contact

No. 2 Wenbo Road, Zhangqiu District, Jinan, Shandong

Qilu Normal University · Genelibs Bioinformatics Lab

750 Shunhua Rd, Jinan

2F, Bldg F, University Science Park

Tel: 0531-88819269

WeChat Official Account

Follow our WeChat subscription account for real-time updates and the latest in medical and biological research.


Business Email

E-mail: [email protected]

Copyright © 2026.Genelibs Jinan Honghui Biotechnology Co., Ltd. 鲁ICP备20007159号-2