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PMID: 2446331 Published · ppublish English Journal Article Research Support, U.S. Gov't, P.H.S.

Modulation of sodium-channel mRNA levels in rat skeletal muscle.

Proceedings of the National Academy of Sciences of the United States of America ·Vol. 84 ·No. 23 ·1987-12-00 ·Pages 8721-5

Cooperman SS, Grubman SA, Barchi RL, Goodman RH, Mandel G

Abstract

Action potentials in many types of excitable cells result from changes in permeability to Na ions. Although these permeability changes in nerve and muscle are mediated by voltage-gated Na channels that are functionally similar, we found that the Na-channel gene expressed in skeletal muscle is different from the genes coding for two Na channels (type I and type II) in brain. Despite the structural differences between muscle and brain Na-channel genes, a cDNA clone derived from rat brain hybridizes to skeletal muscle Na-channel mRNA of approximately 9.5 kilobases. We used this cDNA probe to measure changes in Na-channel mRNA levels in skeletal muscle during development and following denervation. By blot hybridization analysis of electrophoretically fractionated RNA, we found that Na-channel mRNA can be detected as early as embryonic day 17 and that mRNA levels increase 2-fold between birth and postnatal day 35. Denervation of adult muscle causes a further 2- to 3-fold increase in muscle Na-channel mRNA levels, suggesting that expression of Na-channel genes in fast-twitch muscle may be regulated by the state of innervation.

MeSH Terms
Age Factors Animals Gene Expression Regulation Ion Channels/physiology Muscle Denervation Muscle Development Muscles/physiology Nucleic Acid Hybridization RNA, Messenger/genetics Rats Sodium/physiology
Chemicals
Ion Channels RNA, Messenger Sodium
Authors & Affiliations
5 authors, click to expand affiliations / ORCID
Cooperman S S
Department of Medicine, Tufts-New England Medical Center, Boston, MA 02111.
Grubman S A
Barchi R L
Goodman R H
Mandel G
References (27)
27 references, click to expand
  1. Studies on tetrodotoxin resistant action potentials in denervated skeletal muscle.
    Acta Physiol Scand. 1971 Nov;83(3):382-8 PMID: 5134176
  2. Action potential generation in denervated rat skeletal muscle. II. The action of tetrodotoxin.
    Acta Physiol Scand. 1971 May;82(1):70-8 PMID: 4327036
  3. Kinetic and pharmacological properties of the sodium channel of frog skeletal muscle.
    J Gen Physiol. 1976 Mar;67(3):309-23 PMID: 4577
  4. A new method for sequencing DNA.
    Proc Natl Acad Sci U S A. 1977 Feb;74(2):560-4 PMID: 265521
  5. Screening lambdagt recombinant clones by hybridization to single plaques in situ.
    Science. 1977 Apr 8;196(4286):180-2 PMID: 322279
  6. Isolation of biologically active ribonucleic acid from sources enriched in ribonuclease.
    Biochemistry. 1979 Nov 27;18(24):5294-9 PMID: 518835
  7. Voltage-clamp experiments in normal and denervated mammalian skeletal muscle fibres.
    J Physiol. 1980 Sep;306:377-410 PMID: 6257898
  8. Tetrodotoxin-insensitive sodium channels. Ion flux studies of neurotoxin action in a clonal rat muscle cell line.
    J Biol Chem. 1981 Jun 25;256(12):6213-22 PMID: 6113244
  9. Tetrodotoxin-sensitive sodium channels in rat muscle cells developing in vitro.
    J Biol Chem. 1983 Feb 25;258(4):2488-95 PMID: 6296148
  10. Na+ channels with high and low affinity tetrodotoxin binding sites in the mammalian skeletal muscle cell. Difference in functional properties and sequential appearance during rat skeletal myogenesis.
    J Biol Chem. 1983 Jun 25;258(12):7256-9 PMID: 6305931
  11. Differences in the properties of Na+ channels in muscle surface and T-tubular membranes revealed by tetrodotoxin derivatives.
    Pflugers Arch. 1983 Apr;397(1):1-5 PMID: 6306551
  12. Identification of two distinct regulatory regions adjacent to the human beta-interferon gene.
    Cell. 1983 Oct;34(3):865-79 PMID: 6313211
  13. The sodium channel from rat brain. Purification and subunit composition.
    J Biol Chem. 1984 Feb 10;259(3):1667-75 PMID: 6319405
  14. Primary structure of Electrophorus electricus sodium channel deduced from cDNA sequence.
    Nature. 1984 Nov 8-14;312(5990):121-7 PMID: 6209577
  15. Antigenic differences among the voltage-sensitive sodium channels in the peripheral and central nervous systems and skeletal muscle.
    Brain Res. 1985 Apr 1;331(1):145-9 PMID: 2580596
  16. Purification and functional reconstitution of the voltage-sensitive sodium channel from rabbit T-tubular membranes.
    J Biol Chem. 1985 May 25;260(10):6341-7 PMID: 2581954
  17. Muscle denervation increases the levels of two mRNAs coding for the acetylcholine receptor alpha-subunit.
    J Neurosci. 1985 Sep;5(9):2553-8 PMID: 4032011
  18. Thyrotropin-releasing hormone precursor: characterization in rat brain.
    Science. 1986 Jan 10;231(4734):159-61 PMID: 3079917
  19. Existence of distinct sodium channel messenger RNAs in rat brain.
    Nature. 1986 Mar 13-19;320(6058):188-92 PMID: 3754035
  20. Discrimination of muscle and neuronal Na-channel subtypes by binding competition between [3H]saxitoxin and mu-conotoxins.
    Proc Natl Acad Sci U S A. 1986 Jul;83(14):5321-5 PMID: 2425365
  21. Functional differences between two classes of sodium channels in developing rat skeletal muscle.
    Science. 1986 Jul 18;233(4761):361-4 PMID: 2425432
  22. Messenger RNA coding for only the alpha subunit of the rat brain Na channel is sufficient for expression of functional channels in Xenopus oocytes.
    Proc Natl Acad Sci U S A. 1986 Oct;83(19):7503-7 PMID: 2429308
  23. Patch clamp measurements on Xenopus laevis oocytes: currents through endogenous channels and implanted acetylcholine receptor and sodium channels.
    Pflugers Arch. 1986 Dec;407(6):577-88 PMID: 2432468
  24. Monoclonal antibodies against the voltage-sensitive sodium channel from rat skeletal muscle: mapping antibody binding sites.
    J Neurochem. 1987 Mar;48(3):773-8 PMID: 2433396
  25. Localization of sodium channel subtypes in adult rat skeletal muscle using channel-specific monoclonal antibodies.
    J Neurosci. 1987 Sep;7(9):2957-66 PMID: 2442326
  26. Measurement of current-voltage relations in the membrane of the giant axon of Loligo.
    J Physiol. 1952 Apr;116(4):424-48 PMID: 14946712
  27. Tetrodotoxin-resistant action potentials in newborn rat muscle.
    Nat New Biol. 1973 Jun 6;243(127):191-2 PMID: 4514634
Article Info
Journal
Proceedings of the National Academy of Sciences of the United States of America
Abbr.
Proc Natl Acad Sci U S A
ISSN
0027-8424
DOI
10.1073/pnas.84.23.8721
Published
1987-12-00
Pages
8721-5
Language
English
Region
United States
NLM ID
7505876
PMCID
PMC299618
Subset
IM
Grants
NINDS NIH HHS · NS22518 · United States
External Links
PubMed source DOI: 10.1073/pnas.84.23.8721 PMC Full Text View journal details
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