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PMID: 2446864 Published · ppublish English Journal Article Research Support, Non-U.S. Gov't

Characterization of binding properties of the myelin-associated glycoprotein to extracellular matrix constituents.

The EMBO journal ·Vol. 6 ·No. 10 ·1987-10-00 ·Pages 2875-83

Fahrig T, Landa C, Pesheva P, Kühn K, Schachner M

Abstract

The myelin-associated glycoprotein (MAG) can be obtained from adult mouse brain from detergent-lysates of a crude membrane fraction as a 96-100 kd form (detergent solubilized MAG), and from 100,000 g supernatants of homogenates as a 90-96 kd form (soluble MAG). The soluble form distributes into the Triton X-114-poor aqueous phase, while detergent-solubilized MAG predominantly enters the Triton X-114-rich phase. Both molecular forms bind to heparin in hypo- and isotonic buffers. Soluble MAG binds to several collagens (type G, I, II, III, IV, V, VI, IX) with a kd of 5.7 X 10(-8) M for collagen type IX and 2.0 X 10(-7) for collagen type IV. Binding of 125I-labeled MAG to collagen G can be completely inhibited by unlabeled MAG and collagen G, but not by heat-denatured collagen. MAG does not bind to itself, laminin, fibronectin, or the neural cell adhesion molecules L1 and N-CAM. Binding of MAG to collagen G is most effectively blocked by a high molecular weight dextran sulfate, heparan sulfate and heparin, with chondroitin sulfate and a low molecular weight dextran sulfate being less potent blockers. These findings are in agreement with previous observations on the localization of MAG in basal lamina and interstitial collagens of the sciatic nerve in situ.

MeSH Terms
Animals Antibodies, Monoclonal Antigen-Antibody Complex Antigens, Surface Brain/metabolism Cell Adhesion Cell Adhesion Molecules Chromatography, Affinity Collagen/metabolism Extracellular Matrix/metabolism Fibronectins/metabolism Heparin/metabolism Humans Kinetics Mice Mice, Inbred Strains Myelin Proteins/isolation & purification,metabolism Myelin-Associated Glycoprotein Protein Binding Radioimmunoassay
Chemicals
Antibodies, Monoclonal Antigen-Antibody Complex Antigens, Surface Cell Adhesion Molecules Fibronectins Myelin Proteins Myelin-Associated Glycoprotein Heparin Collagen
Authors & Affiliations
5 authors, click to expand affiliations / ORCID
Fahrig T
Department of Neurobiology, University of Heidelberg, FRG.
Landa C
Pesheva P
Kühn K
Schachner M
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Article Info
Journal
The EMBO journal
Abbr.
EMBO J
ISSN
0261-4189
Published
1987-10-00
Pages
2875-83
Language
English
Region
England
NLM ID
8208664
PMCID
PMC553721
Subset
IM
External Links
PubMed source PMC Full Text View journal details
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