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PMID: 2449422 Published · ppublish English Comparative Study Journal Article Research Support, Non-U.S. Gov't

Molecular cloning, characterization, and nucleotide sequence of an extracellular amylase gene from Aeromonas hydrophila.

Journal of bacteriology ·Vol. 170 ·No. 3 ·1988-03-00 ·Pages 1325-32

Gobius KS, Pemberton JM

Abstract

The structural gene for excreted amylase from Aeromonas hydrophila JMP636 has been cloned within a 2.1-kilobase SmaI fragment of DNA. The amylase gene is transcribed from its own promoter in Escherichia coli, producing a gene product of Mr 49,000. The amylase gene product is secreted to the periplasm of E. coli; however, it is not excreted. Nucleotide sequencing revealed an open reading frame of 1,392 base pairs corresponding to a protein of 464 amino acid residues. A potential signal peptide of 21 amino acid residues is present at the NH2 terminal of the predicted protein. Three regions of homology with other procaryotic and eucaryotic alpha-amylases were detected within the predicted amino acid sequence.

MeSH Terms
Aeromonas/enzymology,genetics Amino Acid Sequence Amylases/genetics Base Sequence Chromosome Deletion Cloning, Molecular Codon DNA Mutational Analysis DNA Restriction Enzymes Escherichia coli/genetics Extracellular Space/enzymology Gene Expression Regulation Genes, Bacterial Molecular Sequence Data Protein Sorting Signals
Chemicals
Codon Protein Sorting Signals DNA Restriction Enzymes Amylases
Authors & Affiliations
2 authors, click to expand affiliations / ORCID
Gobius K S
Department of Microbiology, University of Queensland, St. Lucia, Australia.
Pemberton J M
References (31)
31 references, click to expand
  1. Molecular cloning and expression in Escherichia coli of the structural gene for the hemolytic toxin aerolysin from Aeromonas hydrophila.
    Mol Gen Genet. 1986 Aug;204(2):289-95 PMID: 3020368
  2. Aeromonas hydrophila: ecology and toxigenicity of isolates from an estuary.
    J Appl Bacteriol. 1981 Apr;50(2):359-77 PMID: 7251516
  3. Complete nucleotide sequence of bacteriophage T7 DNA and the locations of T7 genetic elements.
    J Mol Biol. 1983 Jun 5;166(4):477-535 PMID: 6864790
  4. A bacteriophage T7 RNA polymerase/promoter system for controlled exclusive expression of specific genes.
    Proc Natl Acad Sci U S A. 1985 Feb;82(4):1074-8 PMID: 3156376
  5. Characterization of translational initiation sites in E. coli.
    Nucleic Acids Res. 1982 May 11;10(9):2971-96 PMID: 7048258
  6. New M13 vectors for cloning.
    Methods Enzymol. 1983;101:20-78 PMID: 6310323
  7. The pUC plasmids, an M13mp7-derived system for insertion mutagenesis and sequencing with synthetic universal primers.
    Gene. 1982 Oct;19(3):259-68 PMID: 6295879
  8. The nature of information, required for export and sorting, present within the outer membrane protein OmpA of Escherichia coli K-12.
    EMBO J. 1985 Dec 16;4(13A):3593-8 PMID: 3912172
  9. Cloning of enterotoxin gene from Aeromonas hydrophila provides conclusive evidence of production of a cytotonic enterotoxin.
    Infect Immun. 1984 Nov;46(2):435-41 PMID: 6500697
  10. Molecular cloning of eucaryotic genes required for excision repair of UV-irradiated DNA: isolation and partial characterization of the RAD3 gene of Saccharomyces cerevisiae.
    J Bacteriol. 1982 Oct;152(1):323-31 PMID: 6749808
  11. Replacement of the fip gene of Escherichia coli by an inactive gene cloned on a plasmid.
    J Bacteriol. 1984 Sep;159(3):1034-9 PMID: 6384177
  12. How signal sequences maintain cleavage specificity.
    J Mol Biol. 1984 Feb 25;173(2):243-51 PMID: 6423828
  13. Cleavage of structural proteins during the assembly of the head of bacteriophage T4.
    Nature. 1970 Aug 15;227(5259):680-5 PMID: 5432063
  14. Codon usage and transfer RNA contents: organism-specific codon-choice patterns in reference to the isoacceptor contents.
    Cold Spring Harb Symp Quant Biol. 1983;47 Pt 2:1087-97 PMID: 6345068
  15. Patterns of amino acids near signal-sequence cleavage sites.
    Eur J Biochem. 1983 Jun 1;133(1):17-21 PMID: 6852022
  16. Mechanism and control of transcription initiation in prokaryotes.
    Annu Rev Biochem. 1985;54:171-204 PMID: 3896120
  17. Signal peptide amino acid sequences in Escherichia coli contain information related to final protein localization. A multivariate data analysis.
    EMBO J. 1987 Mar;6(3):823-31 PMID: 3556168
  18. Cloning, expression, and mapping of the Aeromonas hydrophila aerolysin gene determinant in Escherichia coli K-12.
    J Bacteriol. 1986 Jul;167(1):368-74 PMID: 3522552
  19. Biochemical and exoenzymatic properties of Aeromonas species.
    Diagn Microbiol Infect Dis. 1985 May;3(3):223-32 PMID: 3888508
  20. Complete nucleotide sequence of a thermophilic alpha-amylase gene: homology between prokaryotic and eukaryotic alpha-amylases at the active sites.
    J Biochem. 1985 Jul;98(1):95-103 PMID: 3876333
  21. Isolation of large bacterial plasmids and characterization of the P2 incompatibility group plasmids pMG1 and pMG5.
    J Bacteriol. 1978 Jul;135(1):227-38 PMID: 97269
  22. Construction of plasmid vectors with unique PstI cloning sites in a signal sequence coding region.
    Gene. 1980 Dec;12(3-4):235-41 PMID: 6265317
  23. Studies on transformation of Escherichia coli with plasmids.
    J Mol Biol. 1983 Jun 5;166(4):557-80 PMID: 6345791
  24. Compilation and analysis of Escherichia coli promoter DNA sequences.
    Nucleic Acids Res. 1983 Apr 25;11(8):2237-55 PMID: 6344016
  25. Nonchromosomal antibiotic resistance in bacteria: genetic transformation of Escherichia coli by R-factor DNA.
    Proc Natl Acad Sci U S A. 1972 Aug;69(8):2110-4 PMID: 4559594
  26. Structure and possible catalytic residues of Taka-amylase A.
    J Biochem. 1984 Mar;95(3):697-702 PMID: 6609921
  27. Correlation between the abundance of Escherichia coli transfer RNAs and the occurrence of the respective codons in its protein genes: a proposal for a synonymous codon choice that is optimal for the E. coli translational system.
    J Mol Biol. 1981 Sep 25;151(3):389-409 PMID: 6175758
  28. DNA sequencing with chain-terminating inhibitors.
    Proc Natl Acad Sci U S A. 1977 Dec;74(12):5463-7 PMID: 271968
  29. Nucleotide sequence and transcriptional analysis of the E. coli ushA gene, encoding periplasmic UDP-sugar hydrolase (5'-nucleotidase): regulation of the ushA gene, and the signal sequence of its encoded protein product.
    Nucleic Acids Res. 1986 May 27;14(10):4325-42 PMID: 3012467
  30. Construction and characterization of new cloning vehicles. II. A multipurpose cloning system.
    Gene. 1977;2(2):95-113 PMID: 344137
  31. A small cosmid for efficient cloning of large DNA fragments.
    Gene. 1980 Nov;11(3-4):291-8 PMID: 6260575
Article Info
Journal
Journal of bacteriology
Abbr.
J Bacteriol
ISSN
0021-9193
DOI
10.1128/jb.170.3.1325-1332.1988
Published
1988-03-00
Pages
1325-32
Language
English
Region
United States
NLM ID
2985120R
PMCID
PMC210910
Subset
IM
Databases
GENBANK
M20401
External Links
PubMed source DOI: 10.1128/jb.170.3.1325-1332.1988 PMC Full Text View journal details
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