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PMID: 2449974 Published · ppublish English Journal Article Research Support, Non-U.S. Gov't

Insulin-dependent diabetes mellitus induced in transgenic mice by ectopic expression of class II MHC and interferon-gamma.

Cell ·Vol. 52 ·No. 5 ·1988-03-11 ·Pages 773-82

Sarvetnick N, Liggitt D, Pitts SL, Hansen SE, Stewart TA

Abstract

We have produced transgenic mouse strains harboring class II major histocompatibility complex or interferon-gamma genes linked to the human insulin promoter. These experiments were designed to investigate the consequences of the expression of immunological effector molecules by nonimmunological cells. In both of these studies we observed the disappearance from the pancreas of the insulin-producing beta cells coinciding with the development of insulin-dependent diabetes mellitus. Transgenic mice expressing both chains of the I-A gene showed progressive atrophy of the islets of Langerhans, whereas mice expressing interferon-gamma suffered an inflammatory destruction of the islets.

MeSH Terms
Animals Blood Glucose/metabolism DNA, Recombinant Diabetes Mellitus, Experimental/genetics,metabolism,pathology Diabetes Mellitus, Type 1/genetics,metabolism,pathology Glycosuria/urine Histocompatibility Antigens Class II/genetics Humans Immunoenzyme Techniques Insulin/analysis,genetics Interferon-gamma/genetics Islets of Langerhans/metabolism,pathology Mice Mice, Inbred BALB C Mice, Transgenic Pancreas/analysis Pedigree Plasmids Promoter Regions, Genetic RNA/analysis RNA, Messenger/genetics
Chemicals
Blood Glucose DNA, Recombinant Histocompatibility Antigens Class II Insulin RNA, Messenger RNA Interferon-gamma
Authors & Affiliations
5 authors, click to expand affiliations / ORCID
Sarvetnick N
Department of Developmental Biology, Genentech, Inc., South San Francisco, California 94080.
Liggitt D
Pitts S L
Hansen S E
Stewart T A
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Article Info
Journal
Cell
Abbr.
Cell
ISSN
0092-8674
DOI
10.1016/0092-8674(88)90414-x
Published
1988-03-11
Pages
773-82
Language
English
Region
United States
NLM ID
0413066
PMCID
PMC7133464
Subset
IM
External Links
PubMed source DOI: 10.1016/0092-8674(88)90414-x PMC Full Text View journal details
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