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PMID: 3935430 Published · ppublish English Journal Article Research Support, Non-U.S. Gov't Research Support, U.S. Gov't, P.H.S.

Tissue-specific, inducible and functional expression of the E alpha d MHC class II gene in transgenic mice.

The EMBO journal ·Vol. 4 ·No. 9 ·1985-09-00 ·Pages 2225-30

Pinkert CA, Widera G, Cowing C, Heber-Katz E, Palmiter RD, Flavell RA, Brinster RL

Abstract

We have introduced the class II E alpha d gene into (C57BL/6 X SJL) F2 mice which do not express their endogenous E alpha gene. The mRNA expression of the E alpha d gene shows the same tissue distribution as the endogenous class II genes except in the case of one mouse, which carried 19 copies of the E alpha d gene. In this mouse expression of E alpha d mRNA was seen in all tissues tested. Expression of the transgene was induced by gamma-interferon in isolated macrophages from the transgenic mice. In addition, fluorescence activated cell sorter (FACS) analysis, mixed lymphocyte response and antigen-presentation experiments showed that the product of the transferred gene is expressed on the cell surface and functions as a major histocompatibility complex restriction element. Transmission of the gene occurred only with female transgenic mice, all males were infertile or did not transmit the gene, suggesting an effect of the transferred DNA sequence on male reproductive function.

MeSH Terms
Animals Cloning, Molecular Crosses, Genetic Female Flow Cytometry Genes Genes, MHC Class II Lymph Nodes/immunology Lymphocytes/immunology Macrophage Activation Macrophages/immunology Major Histocompatibility Complex Male Mice Mice, Inbred Strains RNA, Messenger/genetics Spleen/immunology Transcription, Genetic
Chemicals
RNA, Messenger
Authors & Affiliations
7 authors, click to expand affiliations / ORCID
Pinkert C A
Widera G
Cowing C
Heber-Katz E
Palmiter R D
Flavell R A
Brinster R L
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Article Info
Journal
The EMBO journal
Abbr.
EMBO J
ISSN
0261-4189
Published
1985-09-00
Pages
2225-30
Language
English
Region
England
NLM ID
8208664
PMCID
PMC554489
Subset
IM
Grants
NIAID NIH HHS · AI-16044 · United States
NICHD NIH HHS · HD-09172 · United States
NICHD NIH HHS · HD-17321 · United States
External Links
PubMed source PMC Full Text View journal details
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