Cross-reactive and serotype-specific neutralization epitopes on VP7 of human rotavirus: nucleotide sequence analysis of antigenic mutants selected with monoclonal antibodies.

Journal of virology ·Vol. 62 ·No. 6 ·1988-06-00 ·Pages 1870-4

Taniguchi K, Hoshino Y, Nishikawa K, Green KY, Maloy WL, Morita Y, Urasawa S, Kapikian AZ, Chanock RM, Gorziglia M

Abstract

The neutralization epitopes of human and simian rotavirus protein VP7 were studied by producing six neutralizing monoclonal antibodies (N-MAbs) and using these N-MAbs to select antigenic mutants that resisted neutralization by the N-MAbs used for their selection. Cross-neutralization tests between the N-MAbs and the antibody-selected antigenic mutants identified one cross-reactive and five distinct serotype-specific neutralization epitopes which operationally overlapped one another and constituted a single antigenic site. In addition, the amino acid substitutions in human rotavirus VP7 that are responsible for the antigenic alterations in the mutants selected with anti-VP7 cross-reactive or serotype-specific N-MAbs were identified. All the amino acid substitutions in the antigenic mutants occurred in one of two variable regions: amino acids 87 to 101 and 208 to 221.

MeSH Terms

Amino Acid Sequence Antibodies, Monoclonal/immunology Antibodies, Viral/immunology Antigens, Viral/genetics,immunology Cross Reactions Epitopes Molecular Sequence Data Mutation Neutralization Tests Peptides/chemical synthesis,immunology Rotavirus/genetics,immunology Serotyping Structure-Activity Relationship Viral Proteins/genetics,immunology

Chemicals

Antibodies, Monoclonal Antibodies, Viral Antigens, Viral Epitopes Peptides Viral Proteins

Authors & Affiliations

10 authors, click to expand affiliations / ORCID

Taniguchi K

Laboratory of Infectious Diseases, National Institute of Allergy and Infectious Diseases, Bethesda, Maryland 20892.

Hoshino Y

Nishikawa K

Green K Y

Maloy W L

Morita Y

Urasawa S

Kapikian A Z

Chanock R M

Gorziglia M

References (27)

27 references, click to expand

Genes of human (strain Wa) and bovine (strain UK) rotaviruses that code for neutralization and subgroup antigens.
Virology. 1981 Jul 30;112(2):385-90 PMID: 6266134
Nucleotide sequence of the structural glycoprotein VP7 gene of Nebraska calf diarrhea virus rotavirus: comparison with homologous genes from four strains of human and animal rotaviruses.
Virology. 1985 Mar;141(2):292-8 PMID: 2417410
Three human rotavirus serotypes demonstrated by plaque neutralization of isolated strains.
Infect Immun. 1982 Nov;38(2):781-4 PMID: 6292112
Location and primary structure of a major antigenic site for poliovirus neutralization.
Nature. 1983 Feb 24;301(5902):674-9 PMID: 6186919
Gene coding assignments for growth restriction, neutralization and subgroup specificities of the W and DS-1 strains of human rotavirus.
J Gen Virol. 1983 Feb;64 (Pt 2):313-20 PMID: 6300286
Induction of cross-reactive serum neutralizing antibody to human rotavirus in calves after in utero administration of bovine rotavirus.
J Clin Microbiol. 1983 Sep;18(3):505-8 PMID: 6313744
Protection of infants against rotavirus diarrhoea by RIT 4237 attenuated bovine rotavirus strain vaccine.
Lancet. 1984 May 5;1(8384):977-81 PMID: 6143964
Serotypic similarity and diversity of rotaviruses of mammalian and avian origin as studied by plaque-reduction neutralization.
J Infect Dis. 1984 May;149(5):694-702 PMID: 6202807
Nucleotide sequence of the gene encoding the serotype-specific antigen of human (Wa) rotavirus: comparison with the homologous genes from simian SA11 and UK bovine rotaviruses.
J Virol. 1984 Sep;51(3):860-2 PMID: 6088808
Neutralizing monoclonal antibodies to human rotavirus and indications of antigenic drift among strains from neonates.
J Virol. 1985 Apr;54(1):14-20 PMID: 2579249
A candidate for a new serotype of human rotavirus.
J Virol. 1985 May;54(2):623-4 PMID: 2985825
Passive protection against rotavirus-induced diarrhea by monoclonal antibodies to surface proteins vp3 and vp7.
J Virol. 1986 May;58(2):700-3 PMID: 2422398
Location of the major antigenic sites involved in rotavirus serotype-specific neutralization.
Proc Natl Acad Sci U S A. 1986 May;83(10):3465-8 PMID: 2422651
Protective effect of naturally acquired homotypic and heterotypic rotavirus antibodies.
Lancet. 1986 Aug 23;2(8504):417-21 PMID: 2874413
Conservation of amino acid sequence of VP8 and cleavage region of 84-kDa outer capsid protein among rotaviruses recovered from asymptomatic neonatal infection.
Proc Natl Acad Sci U S A. 1986 Sep;83(18):7039-43 PMID: 3018754
Derivation of neutralizing monoclonal antibodies to human rotaviruses and evidence that an immunodominant neutralization site is shared between serotypes 1 and 3.
Virology. 1986 Oct 30;154(2):302-12 PMID: 2429439
Direct serotyping of human rotavirus in stools by an enzyme-linked immunosorbent assay using serotype 1-, 2-, 3-, and 4-specific monoclonal antibodies to VP7.
J Infect Dis. 1987 Jun;155(6):1159-66 PMID: 3033090
Cross-reactive neutralization epitopes on VP3 of human rotavirus: analysis with monoclonal antibodies and antigenic variants.
J Virol. 1987 May;61(5):1726-30 PMID: 2437322
Rotavirus isolate WI61 representing a presumptive new human serotype.
J Clin Microbiol. 1987 Sep;25(9):1757-62 PMID: 2443534
Comparison of the amino acid sequences of the major neutralization protein of four human rotavirus serotypes.
Virology. 1987 Nov;161(1):153-9 PMID: 2823458
Analysis of serotype-specific neutralization epitopes on VP7 of human rotavirus by the use of neutralizing monoclonal antibodies and antigenic variants.
J Gen Virol. 1988 Feb;69 ( Pt 2):451-8 PMID: 2448418
Infection immunity of piglets to either VP3 or VP7 outer capsid protein confers resistance to challenge with a virulent rotavirus bearing the corresponding antigen.
J Virol. 1988 Mar;62(3):744-8 PMID: 2828669
Prediction of human rotavirus serotype by nucleotide sequence analysis of the VP7 protein gene.
J Virol. 1988 May;62(5):1819-23 PMID: 2833626
Preparation and characterization of neutralizing monoclonal antibodies with different reactivity patterns to human rotaviruses.
J Gen Virol. 1985 May;66 ( Pt 5):1045-53 PMID: 2987395
Antigenic variation and resistance to neutralization in poliovirus type 1.
Science. 1985 Sep 13;229(4718):1090-3 PMID: 2412292
Mutations conferring resistance to neutralization with monoclonal antibodies in type 1 poliovirus can be located outside or inside the antibody-binding site.
J Virol. 1986 Jan;57(1):81-90 PMID: 2416957
Antigenic relationships among some animal rotaviruses: virus neutralization in vitro and cross-protection in piglets.
J Clin Microbiol. 1982 Sep;16(3):495-503 PMID: 6290530

Article Info

Journal

Journal of virology

Abbr.

J Virol

ISSN

0022-538X

DOI

10.1128/JVI.62.6.1870-1874.1988

Published

1988-06-00

Pages

1870-4

Language

English

Region

United States

NLM ID

0113724

PMCID

PMC253268

Subset

External Links

PubMed source DOI: 10.1128/JVI.62.6.1870-1874.1988 PMC Full Text View journal details

Analysis Services

Cross-reactive and serotype-specific neutralization epitopes on VP7 of human rotavirus: nucleotide sequence analysis of antigenic mutants selected with monoclonal antibodies.

Contact

WeChat Official Account