Three human rotavirus serotypes demonstrated by plaque neutralization of isolated strains.

Infection and immunity ·Vol. 38 ·No. 2 ·1982-11-00 ·Pages 781-4

Urasawa S, Urasawa T, Taniguchi K

Abstract

Human rotaviruses were isolated directly from stool specimens of gastroenteritis patients in MA-104 cells in the presence of trypsin. For the plaque assay of the isolated strains, the optimal composition of overlay medium was determined. The antigenicity of the isolated strains was investigated by a plaque neutralization method, using antisera prepared against six strains having different electropherotypes of viral RNA, and three different neutralization serotypes were demonstrated.

MeSH Terms

Antigens, Viral Cell Line Culture Media Humans Neutralization Tests Rotavirus/classification,growth & development,immunology Serotyping Viral Plaque Assay

Chemicals

Antigens, Viral Culture Media

Authors & Affiliations

3 authors, click to expand affiliations / ORCID

Urasawa S

Urasawa T

Taniguchi K

References (13)

13 references, click to expand

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Article Info

Journal

Infection and immunity

Abbr.

Infect Immun

ISSN

0019-9567

DOI

10.1128/iai.38.2.781-784.1982

Published

1982-11-00

Pages

781-4

Language

English

Region

United States

NLM ID

0246127

PMCID

PMC347806

Subset

External Links

PubMed source DOI: 10.1128/iai.38.2.781-784.1982 PMC Full Text View journal details

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Three human rotavirus serotypes demonstrated by plaque neutralization of isolated strains.

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