Rescue and serotypic characterization of noncultivable human rotavirus by gene reassortment.

Infection and immunity ·Vol. 37 ·No. 1 ·1982-07-00 ·Pages 104-9

Greenberg HB, Wyatt RG, Kapikian AZ, Kalica AR, Flores J, Jones R

Abstract

Thirty-three of 50 noncultivable human rotavirus strains from a variety of locations were successfully rescued by gene reassortment. The serotype of each of the 33 strains was investigated by a qualitative cytopathic effect neutralization assay. Nineteen strains resembled the previously characterized human rotavirus serotype Wa, whereas three strains were serologically related to the DS-1 strain. Eleven strains appeared to be serotypically distinct from the Wa and DS-1 strains and thus apparently represent one or more new human rotavirus serotypes.

MeSH Terms

Cytopathogenic Effect, Viral Genes, Viral Humans Methods RNA, Viral/analysis Reoviridae/growth & development Rotavirus/classification,genetics,growth & development Serotyping Viral Plaque Assay

Chemicals

RNA, Viral

Authors & Affiliations

6 authors, click to expand affiliations / ORCID

Greenberg H B

Wyatt R G

Kapikian A Z

Kalica A R

Flores J

Jones R

References (16)

16 references, click to expand

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Article Info

Journal

Infection and immunity

Abbr.

Infect Immun

ISSN

0019-9567

DOI

10.1128/iai.37.1.104-109.1982

Published

1982-07-00

Pages

104-9

Language

English

Region

United States

NLM ID

0246127

PMCID

PMC347496

Subset

External Links

PubMed source DOI: 10.1128/iai.37.1.104-109.1982 PMC Full Text View journal details

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Rescue and serotypic characterization of noncultivable human rotavirus by gene reassortment.

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