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PMID: 2456582 Published · ppublish English Journal Article Research Support, Non-U.S. Gov't

Isolation of cDNAs of scrapie-modulated RNAs by subtractive hybridization of a cDNA library.

Proceedings of the National Academy of Sciences of the United States of America ·Vol. 85 ·No. 15 ·1988-08-00 ·Pages 5738-42

Duguid JR, Rohwer RG, Seed B

Abstract

We have developed a subtractive cloning procedure based on the hybridization of single-stranded cDNA libraries constructed in pi H3M, a vector containing the phage M13 origin of replication. We have used this strategy to isolate three transcripts whose abundance is increased in scrapie-infected brain. DNA sequence analysis showed that they represent glial fibrillary acidic protein, metallothionein II, and the B chain of alpha-crystallin; the latter two may represent a response to stress.

MeSH Terms
Amino Acid Sequence Animals Base Sequence Brain Chemistry Cloning, Molecular Cricetinae Crystallins/genetics DNA/genetics,isolation & purification Electrophoresis, Agar Gel Female Glial Fibrillary Acidic Protein/genetics Mesocricetus Metallothionein/genetics Molecular Sequence Data Nucleic Acid Hybridization RNA/genetics Scrapie/genetics Transcription, Genetic
Chemicals
Crystallins Glial Fibrillary Acidic Protein RNA DNA Metallothionein
Authors & Affiliations
3 authors, click to expand affiliations / ORCID
Duguid J R
GRECC, Edith Nourse Rogers Memorial Veterans Administration Hospital, Bedford, MA 01730.
Rohwer R G
Seed B
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Article Info
Journal
Proceedings of the National Academy of Sciences of the United States of America
Abbr.
Proc Natl Acad Sci U S A
ISSN
0027-8424
DOI
10.1073/pnas.85.15.5738
Published
1988-08-00
Pages
5738-42
Language
English
Region
United States
NLM ID
7505876
PMCID
PMC281836
Subset
IM
Databases
GENBANK
J03847, J03848, J03849
External Links
PubMed source DOI: 10.1073/pnas.85.15.5738 PMC Full Text View journal details
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