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PMID: 2457010 Published · ppublish English Journal Article Research Support, U.S. Gov't, P.H.S.

Excision repair of thymine glycols, urea residues, and apurinic sites in Escherichia coli.

Journal of bacteriology ·Vol. 170 ·No. 8 ·1988-08-00 ·Pages 3359-66

Laspia MF, Wallace SS

Abstract

The genetic requirements for the excision repair of thymine glycols, urea residues, and apurinic (AP) sites were examined by measuring the survival in Escherichia coli mutants of phi X174 replicative form (RF) I transfecting DNA containing selectively introduced lesions. phi X RF I DNA containing thymine glycols was inactivated at a greater rate in mutants deficient in endonuclease III (nth) than in wild-type hosts, suggesting that endonuclease III is involved in the repair of thymine glycols in vivo. phi X RF I DNA containing thymine glycols was also inactivated at a greater rate in mutants that were deficient in both exonuclease III and endonuclease IV (xth nfo) than in wild-type hosts, suggesting that a class II AP endonuclease is required for the in vivo processing of thymine glycols. phi X duplex-transfecting DNA containing urea residues or AP sites was inactivated at a greater rate in xth nfo double mutants than in wild-type, but not single-mutant, hosts, suggesting that exonuclease III or endonuclease IV is required for the repair of these damages and that either activity can substitute for the other. These data are in agreement with the known in vitro substrate specificities of endonuclease III, exonuclease III, and endonuclease IV.

MeSH Terms
Coliphages/genetics DNA Damage DNA Repair DNA, Bacterial/analysis,metabolism DNA-(Apurinic or Apyrimidinic Site) Lyase Deoxyribonuclease (Pyrimidine Dimer) Deoxyribonuclease IV (Phage T4-Induced) Endodeoxyribonucleases/genetics,metabolism Escherichia coli/enzymology,genetics,radiation effects Escherichia coli Proteins Exodeoxyribonucleases/genetics,metabolism Mutation SOS Response, Genetics Substrate Specificity Thymine/analogs & derivatives,analysis,metabolism Transfection Ultraviolet Rays Urea/analysis,metabolism
Chemicals
DNA, Bacterial Escherichia coli Proteins thymine glycol Urea Endodeoxyribonucleases Exodeoxyribonucleases exodeoxyribonuclease III Deoxyribonuclease IV (Phage T4-Induced) endonuclease IV, E coli Deoxyribonuclease (Pyrimidine Dimer) NTH protein, E coli DNA-(Apurinic or Apyrimidinic Site) Lyase Thymine
Authors & Affiliations
2 authors, click to expand affiliations / ORCID
Laspia M F
Department of Microbiology, New York Medical College, Valhalla 10595.
Wallace S S
References (48)
48 references, click to expand
  1. Determination of base sequence in nucleic acids with the electron microscope. V. The thymine-specific reactions of osmium tetroxide with deoxyribonucleic acid and its components.
    Biochemistry. 1966 Jul;5(7):2283-8 PMID: 5959451
  2. A DEOXYRIBONUCLEIC ACID PHOSPHATASE-EXONUCLEASE FROM ESCHERICHIA COLI. II. CHARACTERIZATION OF THE EXONUCLEASE ACTIVITY.
    J Biol Chem. 1964 Jan;239:251-8 PMID: 14114851
  3. Rate of chain breakage at apurinic sites in double-stranded deoxyribonucleic acid.
    Biochemistry. 1972 Sep 12;11(19):3618-23 PMID: 4559796
  4. Contribution of various types of damage to inactivation of a biologically-active double-stranded circular DNA by gamma-radiation.
    Int J Radiat Biol Relat Stud Phys Chem Med. 1973 Feb;23(2):133-50 PMID: 4539670
  5. Mutations simultaneously affecting endonuclease II and exonuclease III in Escherichia coli.
    Proc Natl Acad Sci U S A. 1975 Feb;72(2):688-92 PMID: 1091930
  6. Endonucleolytic incision of x-irradiated deoxyribonucleic acid by extracts of Escherichia coli.
    Proc Natl Acad Sci U S A. 1975 Jun;72(6):1997-2001 PMID: 1094450
  7. An endonuclease from Escherichia coli that introduces single polynucleotide chain scissions in ultraviolet-irradiated DNA.
    J Biol Chem. 1976 Mar 10;251(5):1438-45 PMID: 3498
  8. Methyl methane sulfonate-sensitive mutant of Escherichia coli deficient in an endonuclease specific for apurinic sites in deoxyribonucleic acid.
    J Bacteriol. 1976 May;126(2):646-53 PMID: 177402
  9. Ultraviolet mutagenesis and inducible DNA repair in Escherichia coli.
    Bacteriol Rev. 1976 Dec;40(4):869-907 PMID: 795416
  10. Biological effect of thymine ring saturation in coliphage phiX174-DNA.
    Radiat Res. 1977 Feb;69(2):375-8 PMID: 841010
  11. Endonuclease from Escherichia coli that acts specifically upon duplex DNA damaged by ultraviolet light, osmium tetroxide, acid, or x-rays.
    J Biol Chem. 1977 May 10;252(9):2802-7 PMID: 16001
  12. A new endonuclease from Escherichia coli acting at apurinic sites in DNA.
    J Biol Chem. 1977 May 10;252(9):2808-14 PMID: 16002
  13. Thymine fragment damage retained in the DNA polynucleotide chain after gamma irradiation in aerated solutions. II.
    Radiat Res. 1977 Nov;72(2):190-200 PMID: 918256
  14. A DNase for apurinic/apyrimidinic sites associated with exonuclease III of Hemophilus influenzae.
    J Biol Chem. 1978 May 10;253(9):2990-9 PMID: 305919
  15. Ultraviolet light-induced recovery in Escherichia coli of gamma-irradiated bacteriophage DNA.
    Radiat Res. 1979 Nov;80(2):398-408 PMID: 159466
  16. DNA N-glycosylases and UV repair.
    Nature. 1980 Sep 18;287(5779):203-8 PMID: 6253795
  17. Apurinic/apyrimidinic endonucleases in repair of pyrimidine dimers and other lesions in DNA.
    Proc Natl Acad Sci U S A. 1980 Aug;77(8):4602-6 PMID: 6254032
  18. Identification of the cis-thymine glycol moiety in oxidized deoxyribonucleic acid.
    Biochemistry. 1981 Feb 17;20(4):750-4 PMID: 7011371
  19. Depurination causes mutations in SOS-induced cells.
    Proc Natl Acad Sci U S A. 1981 Mar;78(3):1773-7 PMID: 6453349
  20. Role of exonuclease III in the base excision repair of uracil-containing DNA.
    J Bacteriol. 1982 Jul;151(1):351-7 PMID: 6282808
  21. Escherichia coli xth mutants are hypersensitive to hydrogen peroxide.
    J Bacteriol. 1983 Feb;153(2):1079-82 PMID: 6337115
  22. Inducible repair of thymine ring saturation damage in phi X174 DNA.
    Radiat Res. 1983 Mar;93(3):609-12 PMID: 6222390
  23. Studies on transformation of Escherichia coli with plasmids.
    J Mol Biol. 1983 Jun 5;166(4):557-80 PMID: 6345791
  24. Monoclonal antibody to DNA containing thymine glycol.
    Mutat Res. 1983 Aug;112(4):191-200 PMID: 6888408
  25. Characterization of the Escherichia coli X-ray endonuclease, endonuclease III.
    Biochemistry. 1983 Aug 16;22(17):4071-81 PMID: 6351916
  26. Detection and repair of DNA base damages produced by ionizing radiation.
    Environ Mutagen. 1983;5(5):769-88 PMID: 6352254
  27. DNA glycosylase activities for thymine residues damaged by ring saturation, fragmentation, or ring contraction are functions of endonuclease III in Escherichia coli.
    J Biol Chem. 1984 May 10;259(9):5543-8 PMID: 6371006
  28. Mutagenesis and inducible responses to deoxyribonucleic acid damage in Escherichia coli.
    Microbiol Rev. 1984 Mar;48(1):60-93 PMID: 6371470
  29. Properties of antibodies to thymine glycol, a product of the radiolysis of DNA.
    Radiat Res. 1984 Mar;97(3):499-510 PMID: 6729026
  30. Endonuclease III (nth) mutants of Escherichia coli.
    Proc Natl Acad Sci U S A. 1985 Jan;82(2):474-8 PMID: 2982160
  31. Thymine lesions produced by ionizing radiation in double-stranded DNA.
    Biochemistry. 1985 Jul 16;24(15):4018-22 PMID: 3902079
  32. The role of specific DNA base damages in the X-ray-induced inactivation of bacteriophage PM2.
    Mutat Res. 1985 Nov;146(3):229-41 PMID: 2997600
  33. Possible role for thymine glycol in the selective inhibition of DNA synthesis on oxidized DNA templates.
    Cancer Res. 1985 Dec;45(12 Pt 1):6113-8 PMID: 3904979
  34. Thymine glycols and urea residues in M13 DNA constitute replicative blocks in vitro.
    Nucleic Acids Res. 1985 Nov 25;13(22):8035-52 PMID: 3906566
  35. Exonuclease III recognizes urea residues in oxidized DNA.
    Proc Natl Acad Sci U S A. 1985 Dec;82(24):8354-8 PMID: 3001698
  36. Sequence dependence for bypass of thymine glycols in DNA by DNA polymerase I.
    Nucleic Acids Res. 1986 Jan 24;14(2):1045-61 PMID: 3945552
  37. Thymine glycol lesions terminate chain elongation by DNA polymerase I in vitro.
    Nucleic Acids Res. 1986 Jan 24;14(2):737-49 PMID: 3511447
  38. Chemical changes induced in DNA by ionizing radiation.
    Prog Nucleic Acid Res Mol Biol. 1985;32:115-54 PMID: 3003798
  39. Mechanism of action of a mammalian DNA repair endonuclease.
    Biochemistry. 1986 Apr 22;25(8):2212-20 PMID: 2423122
  40. Formation of cytosine glycol and 5,6-dihydroxycytosine in deoxyribonucleic acid on treatment with osmium tetroxide.
    Biochem J. 1986 Apr 15;235(2):531-6 PMID: 3741404
  41. Photoalkylated DNA and ultraviolet-irradiated DNA are incised at cytosines by endonuclease III.
    Nucleic Acids Res. 1986 Aug 26;14(16):6621-31 PMID: 3529039
  42. Exonuclease III and endonuclease IV remove 3' blocks from DNA synthesis primers in H2O2-damaged Escherichia coli.
    Proc Natl Acad Sci U S A. 1986 Oct;83(20):7731-5 PMID: 2429316
  43. Endonuclease IV (nfo) mutant of Escherichia coli.
    J Bacteriol. 1986 Dec;168(3):1120-7 PMID: 2430946
  44. Radiation damage to phi X174 DNA and biological effects.
    Radiat Environ Biophys. 1986;25(3):159-73 PMID: 3025919
  45. Escherichia coli endonuclease III is not an endonuclease but a beta-elimination catalyst.
    Biochem J. 1987 Mar 1;242(2):565-72 PMID: 2439070
  46. The biological consequences of oxidized DNA bases.
    Br J Cancer Suppl. 1987 Jun;8:118-28 PMID: 3477282
  47. Mechanism of action of Escherichia coli endonuclease III.
    Biochemistry. 1987 Dec 15;26(25):8200-6 PMID: 3327518
  48. Effect of growth conditions on the formation of the relaxation complex of supercoiled ColE1 deoxyribonucleic acid and protein in Escherichia coli.
    J Bacteriol. 1972 Jun;110(3):1135-46 PMID: 4555406
Article Info
Journal
Journal of bacteriology
Abbr.
J Bacteriol
ISSN
0021-9193
DOI
10.1128/jb.170.8.3359-3366.1988
Published
1988-08-00
Pages
3359-66
Language
English
Region
United States
NLM ID
2985120R
PMCID
PMC211302
Subset
IM
Grants
NCI NIH HHS · CA 33657 · United States
External Links
PubMed source DOI: 10.1128/jb.170.8.3359-3366.1988 PMC Full Text View journal details
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