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PMID: 6453349 Published · ppublish English Journal Article Research Support, U.S. Gov't, Non-P.H.S. Research Support, U.S. Gov't, P.H.S.

Depurination causes mutations in SOS-induced cells.

Proceedings of the National Academy of Sciences of the United States of America ·Vol. 78 ·No. 3 ·1981-03-00 ·Pages 1773-7

Schaaper RM, Loeb LA

Abstract

Introduction of apurinic sites into phi X174 am3 DNA leads to loss of biological activity when measured in a transfection assay. For single-stranded DNA, approximately one apurinic site constitutes a lethal hit; for double-stranded (RFI) DNA, approximately 3.5 hits per strand are lethal. When the reversion frequency of am3 DNA is measured, no increase due to depurination is observed above the background level. However, a large increase in reversion frequency is observed when the same DNA is assayed by using spheroplasts derived from bacteria previously exposed to UV light. The results suggest that apurinic sites are impediments to a replicating DNA polymerase; however, nucleotides can be incorporated opposite these sites under SOS-induced conditions. We estimate the frequency of mutagenesis per apurinic site to be less than 1 in 1400 in normal spheroplasts and 1 in 100 in SOS-induced spheroplasts.

MeSH Terms
Apurinic Acid/metabolism Bacteriophage phi X 174/genetics DNA Repair DNA Replication DNA, Viral/genetics Escherichia coli/genetics Kinetics Mutation Polynucleotides/metabolism Transfection Virus Replication
Chemicals
DNA, Viral Polynucleotides Apurinic Acid
Authors & Affiliations
2 authors, click to expand affiliations / ORCID
Schaaper R M
Loeb L A
References (35)
35 references, click to expand
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Article Info
Journal
Proceedings of the National Academy of Sciences of the United States of America
Abbr.
Proc Natl Acad Sci U S A
ISSN
0027-8424
DOI
10.1073/pnas.78.3.1773
Published
1981-03-00
Pages
1773-7
Language
English
Region
United States
NLM ID
7505876
PMCID
PMC319216
Subset
IM
Grants
NIA NIH HHS · AG-0751 · United States
NCI NIH HHS · CA-24498 · United States
NCI NIH HHS · CA-24845 · United States
External Links
PubMed source DOI: 10.1073/pnas.78.3.1773 PMC Full Text View journal details
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