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PMID: 2457921 Published · ppublish English Journal Article Research Support, Non-U.S. Gov't

Monoclonal antibodies against human immunodeficiency virus (HIV) type 2 core proteins: cross-reactivity with HIV type 1 and simian immunodeficiency virus.

Proceedings of the National Academy of Sciences of the United States of America ·Vol. 85 ·No. 18 ·1988-09-00 ·Pages 6939-43

Minassian AA, Kalyanaraman VS, Gallo RC, Popovic M

Abstract

Four mouse monoclonal antibodies were developed after immunization with one human immunodeficiency virus (HIV) type 2 isolate and were tested for reactivity with different HIV-1, HIV-2, and simian immunodeficiency virus (SIV) isolates in an immunofluorescence assay and by immunological blot analysis. One of them, an anti-capsid (p24) antibody, called R1C7, reacted with all HIV-1, HIV-2, and SIV isolates tested, thus identifying an epitope shared by all HIV and SIV. Another anti-capsid antibody, named A4F6, reacted with three HIV-2 isolates (HIV-2NIH-Z, LAV-2Rod, and LK001 ST9), some SIV isolates (STLV-IIIAGM, SIV-251, and SIV-309), but no HIV-1 isolates. Two anti-matrix (p16) antibodies, named R5C4 and R5F6, reacted strongly only with the HIV-2 isolates. The use of these monoclonal antibodies for rapid discrimination and identification of acquired immunodeficiency syndrome-related retroviruses is discussed.

MeSH Terms
Animals Antibodies, Monoclonal Capsid/immunology Cross Reactions Epitopes/analysis HIV/immunology HIV Core Protein p24 Haplorhini Mice Monkey Diseases/microbiology Retroviridae/immunology Retroviridae Proteins/immunology
Chemicals
Antibodies, Monoclonal Epitopes HIV Core Protein p24 Retroviridae Proteins
Authors & Affiliations
4 authors, click to expand affiliations / ORCID
Minassian A A
Laboratory of Tumor Cell Biology, National Cancer Institute, Bethesda, MD 20892.
Kalyanaraman V S
Gallo R C
Popovic M
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Article Info
Journal
Proceedings of the National Academy of Sciences of the United States of America
Abbr.
Proc Natl Acad Sci U S A
ISSN
0027-8424
DOI
10.1073/pnas.85.18.6939
Published
1988-09-00
Pages
6939-43
Language
English
Region
United States
NLM ID
7505876
PMCID
PMC282094
Subset
IM
External Links
PubMed source DOI: 10.1073/pnas.85.18.6939 PMC Full Text View journal details
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