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PMID: 2458920 Published · ppublish English Journal Article Research Support, Non-U.S. Gov't

Small finger protein of avian and murine retroviruses has nucleic acid annealing activity and positions the replication primer tRNA onto genomic RNA.

The EMBO journal ·Vol. 7 ·No. 6 ·1988-06-00 ·Pages 1777-83

Prats AC, Sarih L, Gabus C, Litvak S, Keith G, Darlix JL

Abstract

Retrovirus virions carry a diploid genome associated with a large number of small viral finger protein molecules which are required for encapsidation. Our present results show that finger protein p12 of Rous sarcoma virus (RSV) and p10 of murine leukaemia virus (MuLV) positions replication primer tRNA on the replication initiation site (PBS) at the 5' end of the RNA genome. An RSV mutant with a Val-Pro insertion in the finger motif of p12 is able to partially encapsidate genomic RNA but is not infectious because mutated p12 is incapable of positioning the replication primer, tRNATrp. Since all known replication competent retroviruses, and the plant virus CaMV, code for finger proteins analogous to RSV p12 or MuLV p10, the initial stage of reverse transcription in avian, mammalian and human retroviruses and in CaMV is probably controlled in an analogous way.

MeSH Terms
Avian Sarcoma Viruses/genetics,physiology DNA, Viral/biosynthesis Gene Products, gag Moloney murine leukemia virus/genetics,physiology RNA/metabolism RNA, Transfer, Amino Acid-Specific/metabolism RNA, Transfer, Trp/metabolism RNA, Viral/metabolism RNA-Directed DNA Polymerase/metabolism Retroviridae Proteins/physiology Virus Replication
Chemicals
DNA, Viral Gene Products, gag RNA primers RNA, Transfer, Amino Acid-Specific RNA, Transfer, Trp RNA, Viral Retroviridae Proteins RNA RNA-Directed DNA Polymerase
Authors & Affiliations
6 authors, click to expand affiliations / ORCID
Prats A C
Labo-Retro, CRBGC-CNRS, Toulouse, France.
Sarih L
Gabus C
Litvak S
Keith G
Darlix J L
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Article Info
Journal
The EMBO journal
Abbr.
EMBO J
ISSN
0261-4189
Published
1988-06-00
Pages
1777-83
Language
English
Region
England
NLM ID
8208664
PMCID
PMC457168
Subset
IM
External Links
PubMed source PMC Full Text View journal details
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