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PMID: 6197537 Published · ppublish English Journal Article Research Support, Non-U.S. Gov't Research Support, U.S. Gov't, P.H.S.

Two base changes restore infectivity to a noninfectious molecular clone of Moloney murine leukemia virus (pMLV-1).

Journal of virology ·Vol. 49 ·No. 1 ·1984-01-00 ·Pages 214-22

Miller AD, Verma IM

Abstract

The complete nucleotide sequence of a molecular clone of Moloney murine leukemia virus (pMLV-1) has previously been reported (Shinnick et al., Nature [London] 293:543-548, 1981). However, pMLV-1 does not generate infectious virus after transfection into cells (Berns et al., J. Virol. 36:254-263, 1980). The lesion in pMLV-1 has been localized by determining the biological activity of recombinants containing DNA from an infectious clone of Moloney murine leukemia virus (pMLV-48) and pMLV-1. Replacement of a 1.0-kilobase pair region which spans the gag-pol junction of pMLV-1 with the corresponding DNA fragment from the infectious clone restores its infectivity. Nucleotide sequence analysis of this fragment obtained from the infectious clone (pMLV-48) and pMLV-1 reveals two single base pair changes, one in the p30gag gene and the other in the 5' end of the pol gene. The mutation in the pol gene does not affect the production of infectious virus but renders them XC negative, whereas the mutation in the gag gene appears to be lethal. The complete nucleotide sequence of an infectious clone of Moloney murine leukemia virus can now be deduced.

MeSH Terms
Base Sequence DNA, Recombinant DNA, Viral/genetics Gene Products, gag Genes Genes, Viral Moloney murine leukemia virus/genetics Mutation RNA, Viral/genetics RNA-Directed DNA Polymerase/genetics Viral Proteins/genetics Virus Replication
Chemicals
DNA, Recombinant DNA, Viral Gene Products, gag RNA, Viral Viral Proteins RNA-Directed DNA Polymerase
Authors & Affiliations
2 authors, click to expand affiliations / ORCID
Miller A D
Verma I M
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Article Info
Journal
Journal of virology
Abbr.
J Virol
ISSN
0022-538X
DOI
10.1128/JVI.49.1.214-222.1984
Published
1984-01-00
Pages
214-22
Language
English
Region
United States
NLM ID
0113724
PMCID
PMC255444
Subset
IM
Databases
GENBANK
J02255, J02256, J02257
External Links
PubMed source DOI: 10.1128/JVI.49.1.214-222.1984 PMC Full Text View journal details
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