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PMID: 2460406 Published · ppublish English Journal Article Research Support, Non-U.S. Gov't Research Support, U.S. Gov't, Non-P.H.S.

Characterization of the major outer membrane antigens of Treponema hyodysenteriae.

Infection and immunity ·Vol. 56 ·No. 12 ·1988-12-00 ·Pages 3032-9

Wannemuehler MJ, Hubbard RD, Greer JM

Abstract

Outer membrane extracts of Treponema hyodysenteriae were used to evaluate the antibody responses in immunized or convalescent pigs. Western blot (immunoblot) analysis identified antibodies in sera reactive with 14- to 19-kilodalton (kDa) antigens. Reactivity against these antigens could be removed only by absorption of sera with butanol-water-extracted endotoxin from the homologous strain of T. hyodysenteriae. Treatment of the outer membrane extracts with 0.1 M sodium meta-periodate, but not with proteinase K, abolished reactivity with both outer membrane and endotoxin antigens (14 and 19 kDa). These results indicate that swine vaccinated with the outer membrane extract of T. hyodysenteriae develop antibody responses to outer membrane antigens qualitatively similar to those of swine convalescing from active infection, especially antibodies against low-molecular-mass antigens. The nature of the 14- to 19-kDa antigens appears consistent with that of treponemal endotoxin and lipopolysaccharide.

MeSH Terms
Animals Antibodies, Bacterial/immunology Antigens, Bacterial/immunology Bacterial Outer Membrane Proteins/immunology Blotting, Western Electrophoresis, Polyacrylamide Gel Endotoxins/immunology Epitopes Lipopolysaccharides/immunology Molecular Weight Swine Treponema/immunology Treponemal Infections/immunology,prevention & control Vaccination
Chemicals
Antibodies, Bacterial Antigens, Bacterial Bacterial Outer Membrane Proteins Endotoxins Epitopes Lipopolysaccharides
Authors & Affiliations
3 authors, click to expand affiliations / ORCID
Wannemuehler M J
Veterinary Medical Research Institute, Iowa State University, Ames 50011.
Hubbard R D
Greer J M
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Article Info
Journal
Infection and immunity
Abbr.
Infect Immun
ISSN
0019-9567
DOI
10.1128/iai.56.12.3032-3039.1988
Published
1988-12-00
Pages
3032-9
Language
English
Region
United States
NLM ID
0246127
PMCID
PMC259696
Subset
IM
External Links
PubMed source DOI: 10.1128/iai.56.12.3032-3039.1988 PMC Full Text View journal details
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