M2 delta, a candidate for the structure lining the ionic channel of the nicotinic cholinergic receptor.

Proceedings of the National Academy of Sciences of the United States of America ·Vol. 85 ·No. 22 ·1988-11-00 ·Pages 8703-7

Oiki S, Danho W, Madison V, Montal M

Abstract

A synthetic 23-mer peptide that mimics the sequence of the putative transmembrane M2 segment of the Torpedo californica acetylcholine receptor (AcChoR) delta subunit--Glu-Lys-Met-Ser-Thr-Ala-Ile-Ser-Val-Leu-Leu-Ala-Gln-Ala-Val-Phe-Leu- Leu-Leu-Thr-Ser-Gln-Arg--forms discrete ionic channels in phosphatidylcholine bilayers. In contrast, a synthetic peptide that mimics the sequence of the putative M1 transmembrane segment of the Torpedo AcChoR delta subunit--Leu-Phe-Tyr-Val-Ile-Asn-Phe-Ile-Thr-Pro-Cys-Val-Leu-Ile-Ser-Phe- Leu-Ala-Ser-Leu-Ala-Phe-Tyr--does not form channels. The synthetic M2 delta channel peptide exhibits features that are characteristic of the authentic AcChoR channel, such as single channel conductances, discrimination of cations over anions, and channel lifetimes for open and closed states in the millisecond time range. Energetic considerations suggest that an aggregate of five amphipathic alpha-helices conforms the channel. Thus, the M2 segment may be a component of the AcChoR channel structure.

MeSH Terms

Amino Acid Sequence Animals Cattle Chickens Ion Channels/physiology Lipid Bilayers Macromolecular Substances Membrane Potentials Mice Models, Molecular Oligopeptides/chemical synthesis Protein Conformation Receptors, Nicotinic/genetics,physiology Species Specificity Torpedo

Chemicals

Ion Channels Lipid Bilayers Macromolecular Substances Oligopeptides Receptors, Nicotinic

Authors & Affiliations

4 authors, click to expand affiliations / ORCID

Oiki S

Roche Institute of Molecular Biology, Nutley, NJ 07110.

Danho W

Madison V

Montal M

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33 references, click to expand

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Article Info

Journal

Proceedings of the National Academy of Sciences of the United States of America

Abbr.

Proc Natl Acad Sci U S A

ISSN

0027-8424

DOI

10.1073/pnas.85.22.8703

Published

1988-11-00

Pages

8703-7

Language

English

Region

United States

NLM ID

7505876

PMCID

PMC282529

Subset

External Links

PubMed source DOI: 10.1073/pnas.85.22.8703 PMC Full Text View journal details

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M2 delta, a candidate for the structure lining the ionic channel of the nicotinic cholinergic receptor.

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