Home LiteratureArticle Details
PMID: 2470100 Published · ppublish English Comparative Study Journal Article Research Support, Non-U.S. Gov't Research Support, U.S. Gov't, Non-P.H.S. Research Support, U.S. Gov't, P.H.S.

Physical association of CD4 and the T-cell receptor can be induced by anti-T-cell receptor antibodies.

Proceedings of the National Academy of Sciences of the United States of America ·Vol. 86 ·No. 9 ·1989-05-00 ·Pages 3311-5

Rojo JM, Saizawa K, Janeway CA

Abstract

CD4 can physically associate with the CD3-T-cell receptor complex as visualized in cocapping experiments. This association occurs when the T-cell receptor is cross-linked by certain anti-variable region antibodies that appear to induce a conformational change in the receptor such that it associate with CD4. Similar association has been observed in earlier studies with the same cloned helper T cell when the physiological ligand, antigen-class II major histocompatibility complex molecule, is bound by the T-cell receptor. The ability of anti-T-cell receptor antibodies to induce the T-cell receptor-CD4 association correlates with a 100-fold increase in the ability of the antibody to activate the T cell. This suggests that the complex of CD4 and the T-cell receptor act synergistically in T-cell activation, thus readily explaining the commonly observed association of CD4 expression with class II major histocompatibility complex-restricted antigen recognition. This association could also play a role in infection by human immunodeficiency virus.

MeSH Terms
Animals Antibodies, Anti-Idiotypic Antibodies, Monoclonal Antibody Specificity Antigens, Differentiation, T-Lymphocyte/immunology CD3 Complex Cell Line Cricetinae Cross-Linking Reagents Epitopes/immunology Fluorescein-5-isothiocyanate Fluoresceins Fluorescent Antibody Technique Fluorescent Dyes Histocompatibility Antigens Class II/immunology Lymphocyte Activation Macromolecular Substances Mice Rats Receptors, Antigen, T-Cell/immunology T-Lymphocytes, Helper-Inducer/immunology Thiocyanates
Chemicals
Antibodies, Anti-Idiotypic Antibodies, Monoclonal Antigens, Differentiation, T-Lymphocyte CD3 Complex Cross-Linking Reagents Epitopes Fluoresceins Fluorescent Dyes Histocompatibility Antigens Class II Macromolecular Substances Receptors, Antigen, T-Cell Thiocyanates Fluorescein-5-isothiocyanate
Authors & Affiliations
3 authors, click to expand affiliations / ORCID
Rojo J M
Department of Pathology, Yale University School of Medicine, New Haven, CT 06510.
Saizawa K
Janeway C A
References (37)
37 references, click to expand
  1. Molecular dynamics in the membranes of helper T cells.
    Proc Natl Acad Sci U S A. 1988 Nov;85(21):8216-20 PMID: 2973062
  2. The role of L3T4 in T cell activation: L3T4 may be both an Ia-binding protein and a receptor that transduces a negative signal.
    J Mol Cell Immunol. 1986;2(4):179-90 PMID: 3509916
  3. The role of the murine L3T4 molecule in T cell activation: differential effects of anti-L3T4 on activation by monoclonal anti-receptor antibodies.
    J Mol Cell Immunol. 1987;3(2):121-31 PMID: 2978238
  4. Antigens involved in mouse cytolytic T-lymphocyte (CTL)-mediated killing: functional screening and topographic relationship.
    Cell Immunol. 1982 Oct;73(1):1-11 PMID: 6983917
  5. Both a monoclonal antibody and antisera specific for determinants unique to individual cloned helper T cell lines can substitute for antigen and antigen-presenting cells in the activation of T cells.
    J Exp Med. 1983 Sep 1;158(3):836-56 PMID: 6193236
  6. The major histocompatibility complex-restricted antigen receptor on T cells. II. Role of the L3T4 product.
    J Exp Med. 1983 Oct 1;158(4):1077-91 PMID: 6413636
  7. T cell subsets and the recognition of MHC class.
    Immunol Rev. 1983;74:129-42 PMID: 6226585
  8. Characterization of the murine antigenic determinant, designated L3T4a, recognized by monoclonal antibody GK1.5: expression of L3T4a by functional T cell clones appears to correlate primarily with class II MHC antigen-reactivity.
    Immunol Rev. 1983;74:29-56 PMID: 6195085
  9. Characterization of the murine T cell surface molecule, designated L3T4, identified by monoclonal antibody GK1.5: similarity of L3T4 to the human Leu-3/T4 molecule.
    J Immunol. 1983 Nov;131(5):2445-51 PMID: 6415170
  10. The role of L3T4 in recognition of Ia by a cytotoxic, H-2Dd-specific T cell hybridoma.
    J Exp Med. 1984 Apr 1;159(4):1213-24 PMID: 6200564
  11. Triggering of the T3-Ti antigen-receptor complex results in clonal T-cell proliferation through an interleukin 2-dependent autocrine pathway.
    Proc Natl Acad Sci U S A. 1984 Mar;81(5):1509-13 PMID: 6231642
  12. Growth of a cloned helper T cell line induced by a monoclonal antibody specific for the antigen receptor: interleukin 1 is required for the expression of receptors for interleukin 2.
    J Immunol. 1984 Sep;133(3):1339-45 PMID: 6235287
  13. Binding of HTLV-III/LAV to T4+ T cells by a complex of the 110K viral protein and the T4 molecule.
    Science. 1986 Jan 24;231(4736):382-5 PMID: 3001934
  14. Transfer of specificity by murine alpha and beta T-cell receptor genes.
    Nature. 1986 Mar 20-26;320(6059):232-8 PMID: 2421164
  15. Involvement of Lyt-2 and L3T4 in activation of hapten-specific Lyt-2+ L3T4+ T-cell clones.
    Proc Natl Acad Sci U S A. 1986 Apr;83(8):2594-8 PMID: 3085100
  16. Specific antigen-Ia activation of transfected human T cells expressing murine Ti alpha beta-human T3 receptor complexes.
    Nature. 1987 Jan 8-14;325(7000):125-30 PMID: 3027582
  17. Amplification of HTLV-III/LAV infection by antigen-induced activation of T cells and direct suppression by virus of lymphocyte blastogenic responses.
    J Immunol. 1987 Mar 15;138(6):1719-23 PMID: 3493285
  18. Identification of a monoclonal antibody specific for a murine T3 polypeptide.
    Proc Natl Acad Sci U S A. 1987 Mar;84(5):1374-8 PMID: 2950524
  19. Transfection of the CD8 gene enhances T-cell recognition.
    Nature. 1987 Apr 2-8;326(6112):510-1 PMID: 3031507
  20. The L3T4 molecule is part of the helper T-cell antigen/Ia recognition complex.
    Ann Inst Pasteur Immunol. 1987 Jan-Feb;138(1):138-43 PMID: 2953352
  21. Effective activation of resting mouse T lymphocytes by cross-linking submitogenic concentrations of the T cell antigen receptor with either Lyt-2 or L3T4.
    Eur J Immunol. 1987 May;17(5):643-50 PMID: 3108015
  22. Differential effects of antibodies to Lyt-2 and L3T4 on cytolysis by cloned, Ia-restricted T cells expressing both proteins.
    J Immunol. 1987 Jul 15;139(2):380-4 PMID: 3110269
  23. Evidence for a physical association of CD4 and the CD3:alpha:beta T-cell receptor.
    Nature. 1987 Jul 16-22;328(6127):260-3 PMID: 3110627
  24. Reconstitution of MHC class I specificity by transfer of the T cell receptor and Lyt-2 genes.
    Cell. 1987 Aug 14;50(4):545-54 PMID: 2955903
  25. Coclustering of CD4 (L3T4) molecule with the T-cell receptor is induced by specific direct interaction of helper T cells and antigen-presenting cells.
    Proc Natl Acad Sci U S A. 1987 Aug;84(16):5888-92 PMID: 2956608
  26. Interaction between CD4 and class II MHC molecules mediates cell adhesion.
    Nature. 1987 Nov 19-25;330(6145):256-9 PMID: 2823150
  27. The influence of valence on the functional activities of monoclonal anti-L3T4 antibodies. Discrimination of signaling from other effects.
    J Immunol. 1987 Nov 15;139(10):3207-12 PMID: 2960730
  28. Coaggregation of the T-cell receptor with CD4 and other T-cell surface molecules enhances T-cell activation.
    Proc Natl Acad Sci U S A. 1987 Dec;84(24):9209-13 PMID: 3501123
  29. A soluble CD4 protein selectively inhibits HIV replication and syncytium formation.
    Nature. 1988 Jan 7;331(6151):78-81 PMID: 2829023
  30. The biologic activity of anti-T cell receptor V region monoclonal antibodies is determined by the epitope recognized.
    J Immunol. 1988 Feb 15;140(4):1081-8 PMID: 2449495
  31. Comodulation of CD3 and CD4. Evidence for a specific association between CD4 and approximately 5% of the CD3:T cell receptor complexes on helper T lymphocytes.
    J Immunol. 1988 Mar 15;140(6):1732-7 PMID: 2964480
  32. CD4+ T cells: specificity and function.
    Immunol Rev. 1988 Jan;101:39-80 PMID: 2450827
  33. Characterization of a murine monoclonal antibody specific for an allotypic determinant on T cell antigen receptor.
    J Immunol. 1985 Jun;134(6):3994-4000 PMID: 2580901
  34. V beta gene polymorphism and a major polyclonal T cell receptor idiotype.
    Cell. 1985 Aug;42(1):89-92 PMID: 2410144
  35. The isolation and nucleotide sequence of a cDNA encoding the T cell surface protein T4: a new member of the immunoglobulin gene family.
    Cell. 1985 Aug;42(1):93-104 PMID: 2990730
  36. Role of the L3T4-antigen in T cell activation. II. Inhibition of T cell activation by monoclonal anti-L3T4 antibodies in the absence of accessory cells.
    J Immunol. 1985 Oct;135(4):2237-42 PMID: 3897371
  37. Perturbation of the T4 molecule transmits a negative signal to T cells.
    J Exp Med. 1985 Oct 1;162(4):1294-303 PMID: 2413158
Article Info
Journal
Proceedings of the National Academy of Sciences of the United States of America
Abbr.
Proc Natl Acad Sci U S A
ISSN
0027-8424
DOI
10.1073/pnas.86.9.3311
Published
1989-05-00
Pages
3311-5
Language
English
Region
United States
NLM ID
7505876
PMCID
PMC287121
Subset
IM
Grants
NIAID NIH HHS · AI-14579 · United States
NCI NIH HHS · CA-29606 · United States
External Links
PubMed source DOI: 10.1073/pnas.86.9.3311 PMC Full Text View journal details
Analysis Services
Analysis Services

Contact

No. 2 Wenbo Road, Zhangqiu District, Jinan, Shandong

Qilu Normal University · Genelibs Bioinformatics Lab

750 Shunhua Rd, Jinan

2F, Bldg F, University Science Park

Tel: 0531-88819269

WeChat Official Account

Follow our WeChat subscription account for real-time updates and the latest in medical and biological research.


Business Email

E-mail: [email protected]

Copyright © 2026.Genelibs Jinan Honghui Biotechnology Co., Ltd. 鲁ICP备20007159号-2