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PMID: 2472636 Published · ppublish English Journal Article Research Support, Non-U.S. Gov't Research Support, U.S. Gov't, P.H.S.

Inhibition of human immunodeficiency virus (HIV) replication by HIV-trans-activated alpha 2-interferon.

Proceedings of the National Academy of Sciences of the United States of America ·Vol. 86 ·No. 13 ·1989-07-00 ·Pages 4958-62

Bednarik DP, Mosca JD, Raj NB, Pitha PM

Abstract

We have prepared stable cell lines, derived from Vero cells and A3.01 cells, that express a hybrid human alpha 2-interferon gene under control of the human immunodeficiency virus (HIV) long terminal repeat. These cells constitutively produced low levels (50-150 units/ml) of alpha 2-interferon. However, high levels of interferon (10(3) units/ml) could be induced upon trans-activation by the product of the tat gene (pIIIextatIII), and de novo infection by HIV resulted in a moderate increase (400 units/ml) in alpha 2-interferon synthesis. In contrast to the fully permissive HIV replication, in transfected Vero cells or infected A3.01 cells, the transcription and replication of HIV in Vero or A3.01 cells containing the HIV long terminal repeat--alpha 2-interferon hybrid gene (VN89 and A3N89 cells, respectively) was completely inhibited. These data suggest that virus-trans-activated alpha 2-interferon synthesis can be used as a selective inhibitor of HIV replication.

MeSH Terms
Animals Cell Line DNA, Viral/genetics Genetic Vectors HIV/drug effects,physiology Humans Interferon Type I/biosynthesis,genetics,pharmacology Kinetics Plasmids RNA-Directed DNA Polymerase/metabolism Vero Cells Virus Replication/drug effects
Chemicals
DNA, Viral Interferon Type I RNA-Directed DNA Polymerase
Authors & Affiliations
4 authors, click to expand affiliations / ORCID
Bednarik D P
Department of Molecular Biology and Genetics, Johns Hopkins University, Baltimore, MD 21205.
Mosca J D
Raj N B
Pitha P M
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Article Info
Journal
Proceedings of the National Academy of Sciences of the United States of America
Abbr.
Proc Natl Acad Sci U S A
ISSN
0027-8424
DOI
10.1073/pnas.86.13.4958
Published
1989-07-00
Pages
4958-62
Language
English
Region
United States
NLM ID
7505876
PMCID
PMC297535
Subset
IM
Grants
NIAID NIH HHS · AI26123 · United States
External Links
PubMed source DOI: 10.1073/pnas.86.13.4958 PMC Full Text View journal details
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