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PMID: 2474570 Published · ppublish English Journal Article Research Support, Non-U.S. Gov't Research Support, U.S. Gov't, P.H.S.

Membrane cofactor protein of complement is present on human fibroblast, epithelial, and endothelial cells.

The Journal of clinical investigation ·Vol. 84 ·No. 2 ·1989-08-00 ·Pages 538-45

McNearney T, Ballard L, Seya T, Atkinson JP

Abstract

Membrane cofactor protein (MCP) of the complement system is a iC3/C3b binding molecule with cofactor activity that has been identified on all human peripheral blood cells except erythrocytes. Human mononuclear and platelet MCP is dimeric with molecular weights of 68,000 and 63,000 and is expressed in three phenotypic patterns. To further determine its tissue distribution, surface-labeled human fibroblast, epithelial, and endothelial cells and cell lines were assessed for the presence of MCP by iC3 affinity chromatography and by immunoprecipitation with a monospecific anti-MCP rabbit polyclonal antibody. All sources of adult and fetal fibroblast and epithelial cells and cell lines examined and umbilical vein endothelial cells expressed MCP. The molecular weight and phenotypic patterns of MCP were similar to those of peripheral blood cells. MCP was synthesized by fibroblast and epithelial cell lines. Solubilized extracts of these cell lines expressed factor I-dependent cofactor activity for the first cleavage of iC3/C3b which was abrogated by removal of MCP. Expression of MCP was modulated by SV40 transformation of two fetal fibroblast lines. There was a 5- to 10-fold increase in expression of MCP and a preferential expression of the lower species such that the phenotypic designation was changed. The wide tissue distribution and activity profile of MCP suggest that it is likely to play an important role in the regulation of the complement cascade.

MeSH Terms
Antigens, CD CD55 Antigens Cell Line Cell Transformation, Viral Complement System Proteins/analysis,physiology Endothelium, Vascular/analysis Epithelium/analysis Fibroblasts/analysis Humans Membrane Cofactor Protein Membrane Glycoproteins/analysis,biosynthesis,physiology Membrane Proteins/analysis Simian virus 40
Chemicals
Antigens, CD CD46 protein, human CD55 Antigens Membrane Cofactor Protein Membrane Glycoproteins Membrane Proteins Complement System Proteins
Authors & Affiliations
4 authors, click to expand affiliations / ORCID
McNearney T
Howard Hughes Medical Institute Laboratories, Department of Medicine, Washington University School of Medicine, St. Louis, Missouri 63110.
Ballard L
Seya T
Atkinson J P
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Article Info
Journal
The Journal of clinical investigation
Abbr.
J Clin Invest
ISSN
0021-9738
DOI
10.1172/JCI114196
Published
1989-08-00
Pages
538-45
Language
English
Region
United States
NLM ID
7802877
PMCID
PMC548913
Subset
IM
Grants
NIAID NIH HHS · 5R01-AI19642 · United States
NIADDK NIH HHS · AM07279 · United States
NIAMS NIH HHS · T32-AR07279 · United States
External Links
PubMed source DOI: 10.1172/JCI114196 PMC Full Text View journal details
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