Home LiteratureArticle Details
PMID: 2479541 Published · ppublish English Journal Article

The Drosophila hairy protein acts in both segmentation and bristle patterning and shows homology to N-myc.

The EMBO journal ·Vol. 8 ·No. 10 ·1989-10-00 ·Pages 3095-103

Rushlow CA, Hogan A, Pinchin SM, Howe KM, Lardelli M, Ish-Horowicz D

Abstract

The Drosophila segmentation gene, hairy (h), acts to regulate embryonic segmentation and bristle pattern. We present the DNA sequence of the h gene and of h cDNAs, thereby deducing the organization of the h transcripts. The h gene encodes a 337 amino acid protein that acts in both embryonic segmentation and adult bristle patterning. The h protein includes a domain that shows extensive similarity to a domain of the proto-oncogene N-myc that may be involved in DNA binding and/or protein dimerization. We discuss mechanisms of h action as a transcriptional regulator.

MeSH Terms
Amino Acid Sequence Animals Base Sequence Basic Helix-Loop-Helix Transcription Factors Blotting, Northern Cosmids DNA/genetics Drosophila/genetics Drosophila Proteins Genes Insect Hormones Insect Proteins Molecular Sequence Data Mutation Oncogenes Phenotype RNA/genetics Repressor Proteins Restriction Mapping Sequence Homology, Nucleic Acid Transcription Factors/analysis,genetics,physiology Transcription, Genetic Transfection
Chemicals
Basic Helix-Loop-Helix Transcription Factors Drosophila Proteins Insect Hormones Insect Proteins Repressor Proteins Transcription Factors hairy protein, Drosophila hairy protein, insect RNA DNA
Authors & Affiliations
6 authors, click to expand affiliations / ORCID
Rushlow C A
Department of Zoology, University of Oxford, UK.
Hogan A
Pinchin S M
Howe K M
Lardelli M
Ish-Horowicz D
References (61)
61 references, click to expand
  1. opa: a novel family of transcribed repeats shared by the Notch locus and other developmentally regulated loci in D. melanogaster.
    Cell. 1985 Jan;40(1):55-62 PMID: 2981631
  2. Closely related transcripts encoded by the neurogenic gene complex enhancer of split of Drosophila melanogaster.
    EMBO J. 1989 Jan;8(1):203-10 PMID: 2540957
  3. Conservation of a large protein domain in the segmentation gene paired and in functionally related genes of Drosophila.
    Cell. 1986 Dec 26;47(6):1033-40 PMID: 2877747
  4. Cloning sequences from the hairy gene of Drosophila.
    EMBO J. 1984 Mar;3(3):569-73 PMID: 6425055
  5. Comparison of the consensus sequence flanking translational start sites in Drosophila and vertebrates.
    Nucleic Acids Res. 1987 Feb 25;15(4):1353-61 PMID: 3822832
  6. The ovalbumin gene-sequence of putative control regions.
    Nucleic Acids Res. 1980 Jan 11;8(1):127-42 PMID: 6243777
  7. Improved tools for biological sequence comparison.
    Proc Natl Acad Sci U S A. 1988 Apr;85(8):2444-8 PMID: 3162770
  8. Direct interaction between fos and jun nuclear oncoproteins: role of the 'leucine zipper' domain.
    Nature. 1988 Dec 15;336(6200):692-5 PMID: 3143919
  9. Sequence of a Drosophila segmentation gene: protein structure homology with DNA-binding proteins.
    Nature. 1984 Jul 5-11;310(5972):25-31 PMID: 6330566
  10. Site-specific polyadenylation in a cell-free reaction.
    Cell. 1984 Mar;36(3):581-91 PMID: 6230155
  11. Molecular and genetic analysis of the hairy locus in Drosophila.
    Cold Spring Harb Symp Quant Biol. 1985;50:135-44 PMID: 3006986
  12. Glycoproteins.
    Annu Rev Biochem. 1972;41:673-702 PMID: 4563441
  13. c-Jun dimerizes with itself and with c-Fos, forming complexes of different DNA binding affinities.
    Cell. 1988 Dec 2;55(5):917-24 PMID: 3142692
  14. Distantly related sequences in the alpha- and beta-subunits of ATP synthase, myosin, kinases and other ATP-requiring enzymes and a common nucleotide binding fold.
    EMBO J. 1982;1(8):945-51 PMID: 6329717
  15. Expression, function, and regulation of the hairy segmentation protein in the Drosophila embryo.
    Genes Dev. 1988 Jul;2(7):883-90 PMID: 3209072
  16. Structure of the segmentation gene paired and the Drosophila PRD gene set as part of a gene network.
    Cell. 1986 Dec 5;47(5):735-46 PMID: 2877746
  17. Interactions among the gypsy transposable element and the yellow and the suppressor of hairy-wing loci in Drosophila melanogaster.
    Mol Cell Biol. 1986 Jan;6(1):47-53 PMID: 3023836
  18. Three regions upstream from the cap site are required for efficient and accurate transcription of the rabbit beta-globin gene in mouse 3T6 cells.
    Cell. 1983 Mar;32(3):695-706 PMID: 6299573
  19. Mutations affecting segment number and polarity in Drosophila.
    Nature. 1980 Oct 30;287(5785):795-801 PMID: 6776413
  20. False starts in translational control of gene expression.
    Nature. 1985 Aug 15-21;316(6029):580-1 PMID: 4033756
  21. The engrailed locus of Drosophila: structural analysis of an embryonic transcript.
    Cell. 1985 Jan;40(1):37-43 PMID: 3917855
  22. Nucleotide sequence of the human N-myc gene.
    Proc Natl Acad Sci U S A. 1986 Mar;83(6):1772-6 PMID: 2869488
  23. The molecular basis for metameric pattern in the Drosophila embryo.
    Development. 1987 Sep;101(1):1-22 PMID: 2896587
  24. Genetic Analysis of the Hairy Locus in DROSOPHILA MELANOGASTER.
    Genetics. 1985 Nov;111(3):463-86 PMID: 17246301
  25. Spliced early mRNAs of simian virus 40.
    Proc Natl Acad Sci U S A. 1978 Mar;75(3):1274-8 PMID: 206891
  26. Point mutations define a sequence flanking the AUG initiator codon that modulates translation by eukaryotic ribosomes.
    Cell. 1986 Jan 31;44(2):283-92 PMID: 3943125
  27. Regulatory interactions between the segmentation genes fushi tarazu, hairy, and engrailed in the Drosophila blastoderm.
    Cell. 1986 Mar 28;44(6):949-57 PMID: 3955654
  28. Protein import into the cell nucleus.
    Annu Rev Cell Biol. 1986;2:367-90 PMID: 3548772
  29. A transposable P vector that confers selectable G418 resistance to Drosophila larvae.
    EMBO J. 1985 Jan;4(1):167-71 PMID: 16453599
  30. Gene-dose titration analysis in the search of trans-regulatory genes in Drosophila.
    EMBO J. 1982;1(3):307-10 PMID: 7188185
  31. The achaete-scute gene complex of D. melanogaster: conserved domains in a subset of genes required for neurogenesis and their homology to myc.
    Cell. 1987 Jul 31;50(3):415-24 PMID: 3111716
  32. Pattern abnormalities induced by ectopic expression of the Drosophila gene hairy are associated with repression of ftz transcription.
    Cell. 1987 Nov 6;51(3):405-15 PMID: 3664640
  33. A conserved DNA sequence in homoeotic genes of the Drosophila Antennapedia and bithorax complexes.
    Nature. 1984 Mar 29-Apr 4;308(5958):428-33 PMID: 6323992
  34. The leucine zipper: a hypothetical structure common to a new class of DNA binding proteins.
    Science. 1988 Jun 24;240(4860):1759-64 PMID: 3289117
  35. Unidirectional digestion with exonuclease III creates targeted breakpoints for DNA sequencing.
    Gene. 1984 Jun;28(3):351-9 PMID: 6235151
  36. The Hox-1.3 homeo box protein is a sequence-specific DNA-binding phosphoprotein.
    Genes Dev. 1989 Feb;3(2):158-72 PMID: 2565857
  37. Homology between the DNA-binding domain of the GCN4 regulatory protein of yeast and the carboxyl-terminal region of a protein coded for by the oncogene jun.
    Proc Natl Acad Sci U S A. 1987 May;84(10):3316-9 PMID: 3554236
  38. A catalogue of splice junction sequences.
    Nucleic Acids Res. 1982 Jan 22;10(2):459-72 PMID: 7063411
  39. Excess function hairy-wing mutations caused by gypsy and copia insertions within structural genes of the achaete-scute locus of Drosophila.
    Cell. 1986 Jan 31;44(2):303-12 PMID: 3002632
  40. Region-specific alleles of the Drosophila segmentation gene hairy.
    Genes Dev. 1988 Aug;2(8):1037-46 PMID: 3169547
  41. A new DNA binding and dimerization motif in immunoglobulin enhancer binding, daughterless, MyoD, and myc proteins.
    Cell. 1989 Mar 10;56(5):777-83 PMID: 2493990
  42. The invected gene of Drosophila: sequence analysis and expression studies reveal a close kinship to the engrailed gene.
    Genes Dev. 1987 Mar;1(1):19-28 PMID: 2892756
  43. In vitro transcription of the Drosophila engrailed gene.
    Genes Dev. 1988 Jan;2(1):68-81 PMID: 3356339
  44. pEMBL: a new family of single stranded plasmids.
    Nucleic Acids Res. 1983 Mar 25;11(6):1645-55 PMID: 6300771
  45. Genetic analysis of cell heredity in imaginal discs of Drosophila melanogaster.
    Proc Natl Acad Sci U S A. 1971 Sep;68(9):2222-6 PMID: 5002430
  46. DNA binding activities of three murine Jun proteins: stimulation by Fos.
    Cell. 1988 Dec 2;55(5):907-15 PMID: 3142691
  47. The role of the leucine zipper in the fos-jun interaction.
    Nature. 1988 Dec 15;336(6200):646-51 PMID: 2974122
  48. daughterless, a Drosophila gene essential for both neurogenesis and sex determination, has sequence similarities to myc and the achaete-scute complex.
    Cell. 1988 Dec 23;55(6):1061-7 PMID: 3203380
  49. Transcriptional control signals of a eukaryotic protein-coding gene.
    Science. 1982 Jul 23;217(4557):316-24 PMID: 6283634
  50. The bicoid protein is a positive regulator of hunchback transcription in the early Drosophila embryo.
    Nature. 1989 Jan 12;337(6203):138-43 PMID: 2911348
  51. Molecular analysis of ouabain-resistant mutants of the mouse lymphoma cell line L5178Y.
    Mutagenesis. 1987 Sep;2(5):383-9 PMID: 2830454
  52. Molecular characterization of the zerknüllt region of the Antennapedia gene complex in Drosophila.
    Genes Dev. 1987 Dec;1(10):1268-79 PMID: 2892759
  53. Zygotically active genes that affect the spatial expression of the fushi tarazu segmentation gene during early Drosophila embryogenesis.
    Cell. 1986 Apr 11;45(1):113-26 PMID: 3082519
  54. A putative signal peptidase recognition site and sequence in eukaryotic and prokaryotic signal peptides.
    J Mol Biol. 1983 Jun 25;167(2):391-409 PMID: 6345794
  55. The achaete-scute gene complex of Drosophila melanogaster comprises four homologous genes.
    EMBO J. 1988 Aug;7(8):2585-91 PMID: 2461300
  56. Characterization and developmental expression of a Drosophila ras oncogene.
    Mol Cell Biol. 1985 Apr;5(4):885-9 PMID: 3921827
  57. Transcription termination and 3' processing: the end is in site!
    Cell. 1985 Jun;41(2):349-59 PMID: 2580642
  58. A gradient of bicoid protein in Drosophila embryos.
    Cell. 1988 Jul 1;54(1):83-93 PMID: 3383244
  59. The molecular genetics of embryonic pattern formation in Drosophila.
    Nature. 1988 Sep 1;335(6185):25-34 PMID: 2901040
  60. Sequence and structure of the serendipity locus of Drosophila melanogaster. A densely transcribed region including a blastoderm-specific gene.
    J Mol Biol. 1985 Nov 5;186(1):149-66 PMID: 3935797
  61. A model for the tertiary structure of p21, the product of the ras oncogene.
    Science. 1985 Oct 4;230(4721):78-82 PMID: 3898366
Article Info
Journal
The EMBO journal
Abbr.
EMBO J
ISSN
0261-4189
Published
1989-10-00
Pages
3095-103
Language
English
Region
England
NLM ID
8208664
PMCID
PMC401388
Subset
IM
Databases
GENBANK
X15904, X15905
External Links
PubMed source PMC Full Text View journal details
Analysis Services
Analysis Services

Contact

No. 2 Wenbo Road, Zhangqiu District, Jinan, Shandong

Qilu Normal University · Genelibs Bioinformatics Lab

750 Shunhua Rd, Jinan

2F, Bldg F, University Science Park

Tel: 0531-88819269

WeChat Official Account

Follow our WeChat subscription account for real-time updates and the latest in medical and biological research.


Business Email

E-mail: [email protected]

Copyright © 2026.Genelibs Jinan Honghui Biotechnology Co., Ltd. 鲁ICP备20007159号-2