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PMID: 2483252 Published · ppublish English Journal Article Research Support, Non-U.S. Gov't

Evidence for a highly nuclease resistant RNA fragment in prosomes.

Molecular biology reports ·Vol. 13 ·No. 4 ·1988-00-00 ·Pages 207-11

Dineva B, Tomek W, Köhler K, Schmid HP

Abstract

Prosomes, small cytoplasmic particles of mouse erythroblasts were found to contain low molecular weight RNA molecules in the range of 80 nucleotides. Nuclease digestion of prosomes suggests that prosomal proteins cover and protect almost the whole length of their RNA(s). Our results demonstrate clearly that RNA is an intrinsic component of prosomes.

MeSH Terms
Animals Cytoplasm/analysis Electrophoresis, Polyacrylamide Gel Erythroblasts/analysis Mice Mice, Inbred BALB C RNA/analysis Ribonucleases Ribonucleoproteins/analysis
Chemicals
Ribonucleoproteins RNA Ribonucleases
Authors & Affiliations
4 authors, click to expand affiliations / ORCID
Dineva B
Biologisches Institut, Universität Stuttgart, FRG.
Tomek W
Köhler K
Schmid H P
References (14)
14 references, click to expand
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  3. Analysis of cytoplasmic 19 S ring-type particles in Drosophila which contain hsp 23 at normal growth temperature.
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  7. 3'-terminal labelling of RNA with T4 RNA ligase.
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  8. Sea urchin prosome: characterization and changes during development.
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  9. High resolution two-dimensional electrophoresis of basic as well as acidic proteins.
    Cell. 1977 Dec;12(4):1133-41 PMID: 23215
  10. Reconstitution of the U1 small nuclear ribonucleoprotein particle.
    Mol Cell Biol. 1987 Nov;7(11):4030-7 PMID: 2963210
  11. A 20S particle ubiquitous from yeast to human.
    J Mol Evol. 1987;25(2):141-50 PMID: 2443724
  12. On the lability of poly(A) sequences during extraction of messenger RNA from polyribosomes.
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  13. Possible involvement of messenger RNA-associated proteins in protein synthesis.
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  14. The prosome: an ubiquitous morphologically distinct RNP particle associated with repressed mRNPs and containing specific ScRNA and a characteristic set of proteins.
    EMBO J. 1984 Jan;3(1):29-34 PMID: 6200323
Article Info
Journal
Molecular biology reports
Abbr.
Mol Biol Rep
ISSN
0301-4851
DOI
10.1007/BF00788172
Published
1988-00-00
Pages
207-11
Language
English
Region
Netherlands
NLM ID
0403234
Subset
IM
External Links
PubMed source DOI: 10.1007/BF00788172 View journal details
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