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PMID: 6811601 Published · ppublish English Journal Article

Possible involvement of messenger RNA-associated proteins in protein synthesis.

The Journal of cell biology ·Vol. 93 ·No. 3 ·1982-06-00 ·Pages 893-8

Schmid HP, Köhler K, Setyono B

Abstract

Two distinct forms of globin messenger RNA were isolated from mouse spleen cells infected with Friend erythroleukemia virus: polyribosomal messenger ribonucleoprotein particles (15S mRNP), and their corresponding protein-free mRNAs obtained by chemical deproteinization. The translation efficiencies of both messenger forms were assayed in a Krebs II ascites cell-free system. Selective removal of RNA-binding proteins from the ascites cell lysate did not affect globin synthesis when the mRNA was supplied as 15S mRNP; deproteinized mRNA however was not translated. Only in the presence of two fractions of RNA-binding proteins was the protein-free mRNA translated. Some of the RNA-binding proteins have the same molecular weights and isoelectric points as the principal proteins of 15S mRNP.

MeSH Terms
Animals Carrier Proteins/physiology Cell Fractionation Cell-Free System Friend murine leukemia virus Globins/biosynthesis,genetics Leukemia, Experimental/genetics Mice Mice, Inbred BALB C Protein Biosynthesis RNA, Messenger/physiology RNA-Binding Proteins Ribonucleoproteins/analysis Spleen/metabolism
Chemicals
Carrier Proteins RNA, Messenger RNA-Binding Proteins Ribonucleoproteins messenger ribonucleoprotein Globins
Authors & Affiliations
3 authors, click to expand affiliations / ORCID
Schmid H P
Köhler K
Setyono B
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47 references, click to expand
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Article Info
Journal
The Journal of cell biology
Abbr.
J Cell Biol
ISSN
0021-9525
DOI
10.1083/jcb.93.3.893
Published
1982-06-00
Pages
893-8
Language
English
Region
United States
NLM ID
0375356
PMCID
PMC2112157
Subset
IM
External Links
PubMed source DOI: 10.1083/jcb.93.3.893 PMC Full Text View journal details
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