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PMID: 2491017 Published · ppublish English Journal Article Research Support, Non-U.S. Gov't

Noninactivation of a selectable human X-linked gene that complements a murine temperature-sensitive cell cycle defect.

American journal of human genetics ·Vol. 45 ·No. 4 ·1989-10-00 ·Pages 592-8

Brown CJ, Willard HF

Abstract

The human gene A1S9T, which complements the temperature-sensitive cell-cycle defect in the murine cell line tsA1S9 and which has previously been assigned to the X-chromosome short arm, is expressed from the inactive X chromosome in human/tsA1S9 somatic cell hybrids grown at the nonpermissive temperature. The Y chromosome cannot complement the defect; thus, unlike at least two other noninactivated X loci, A1S9T has no functional Y-linked homologue. As A1S9T is readily selectable in somatic cell hybrids with the tsA1S9 mouse line, this marker should be useful in isolating somatic cell hybrids containing inactive X chromosomes, or abnormal X's (active or inactive) retaining the short arm.

MeSH Terms
Animals Cell Cycle/genetics Cells, Cultured Fibroblasts/cytology Glucosephosphate Dehydrogenase/genetics,metabolism Humans Hybrid Cells/cytology Hypoxanthine Phosphoribosyltransferase/genetics,metabolism Karyotyping Methylation Mice Skin/cytology Temperature X Chromosome
Chemicals
Glucosephosphate Dehydrogenase Hypoxanthine Phosphoribosyltransferase
Authors & Affiliations
2 authors, click to expand affiliations / ORCID
Brown C J
Department of Medical Genetics, University of Toronto.
Willard H F
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Article Info
Journal
American journal of human genetics
Abbr.
Am J Hum Genet
ISSN
0002-9297
Published
1989-10-00
Pages
592-8
Language
English
Region
United States
NLM ID
0370475
PMCID
PMC1683489
Subset
IM
External Links
PubMed source PMC Full Text View journal details
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