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PMID: 2491670 Published · ppublish English Journal Article Research Support, Non-U.S. Gov't

Mutational analysis of the conserved domains of a T-region border repeat of Agrobacterium tumefaciens.

Plant molecular biology ·Vol. 13 ·No. 5 ·1989-11-00 ·Pages 523-31

van Haaren MJ, Sedee NJ, de Boer HA, Schilperoort RA, Hooykaas PJ

Abstract

Left- and right-border repeats, which surround the T-region, contain two conserved regions separated by 5 bp that are not conserved. At the onset of T-DNA processing virD-encoded proteins introduce a nick in the largest of these conserved regions (12 bp) at a specific position in the bottom strand between a guanine and thymine nucleotide [2, 33]. In this paper we describe the effect of several site-directed mutations in the right-border repeat on tumorigenicity of Agrobacterium in plants. Our data show that mutations introduced directly around the nick site do not seriously affect the tumorigenicity of Agrobacterium, whereas mutations in the right part of this 12 bp conserved region do so. Furthermore, it appeared that the second conserved region (5 bp) is also essential for border activity and that the distance between the two conserved regions is important to obtain optimal border activity.

Related Genes
vir
MeSH Terms
Base Sequence DNA/genetics DNA Mutational Analysis DNA, Bacterial/genetics Molecular Sequence Data Plants/genetics,microbiology Plasmids Repetitive Sequences, Nucleic Acid Rhizobium/genetics,pathogenicity Transfection
Chemicals
DNA, Bacterial T-DNA DNA
Authors & Affiliations
5 authors, click to expand affiliations / ORCID
van Haaren M J
Department of Plant Molecular Biology, Leiden University, Netherlands.
Sedee N J
de Boer H A
Schilperoort R A
Hooykaas P J
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34 references, click to expand
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Article Info
Journal
Plant molecular biology
Abbr.
Plant Mol Biol
ISSN
0167-4412
DOI
10.1007/BF00027312
Published
1989-11-00
Pages
523-31
Language
English
Region
Netherlands
NLM ID
9106343
Subset
IM
External Links
PubMed source DOI: 10.1007/BF00027312 View journal details
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