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PMID: 2492501 Published · ppublish English Journal Article Research Support, Non-U.S. Gov't Research Support, U.S. Gov't, P.H.S.

Structural and functional analysis of transcriptional control of the Rhodobacter capsulatus puf operon.

Journal of bacteriology ·Vol. 171 ·No. 1 ·1989-01-00 ·Pages 473-82

Adams CW, Forrest ME, Cohen SN, Beatty JT

Abstract

We report data indicating that the Rhodobacter capsulatus puf operon promoter and the site for its oxygen regulation are located more than 700 base pairs upstream from the previously identified puf genes and have identified the nucleotide sequences that constitute these control signals. A model is proposed in which a polycistronic transcript at least 3.4 kilobases in length is initiated near the O2-regulated promoter and is processed posttranscriptionally by endonucleolytic cleavage at multiple sites, yielding discrete mRNA segments that are degraded at different rates. A newly identified gene (pufQ), which includes a hydrophobic domain having some similarity to domains of the products of the pufL and pufM genes, begins 313 nucleotides into the puf transcript and is located entirely within the most rapidly degraded segment of the transcript. A previously identified puf transcript segment encoding structural proteins for photosynthetic membrane complexes persists after degradation of the most 5' region of the transcript and is itself subject to segmentally specific degradation. Our results suggest a model in which differential expression of the multiple genes encoded by the puf operon is at least in part attributable to major differences in the rates of decay of the various segments of puf mRNA.

MeSH Terms
Amino Acid Sequence Bacterial Proteins/genetics Base Sequence Genes Genes, Bacterial Molecular Sequence Data Operon Photosynthetic Reaction Center Complex Proteins Promoter Regions, Genetic Protein Conformation Restriction Mapping Rhodopseudomonas/genetics Transcription, Genetic beta-Galactosidase
Chemicals
Bacterial Proteins Photosynthetic Reaction Center Complex Proteins beta-Galactosidase
Authors & Affiliations
4 authors, click to expand affiliations / ORCID
Adams C W
Department of Genetics, Stanford University School of Medicine, California 94305.
Forrest M E
Cohen S N
Beatty J T
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Article Info
Journal
Journal of bacteriology
Abbr.
J Bacteriol
ISSN
0021-9193
DOI
10.1128/jb.171.1.473-482.1989
Published
1989-01-00
Pages
473-82
Language
English
Region
United States
NLM ID
2985120R
PMCID
PMC209611
Subset
IM
Grants
NIGMS NIH HHS · GM07790 · United States
NIGMS NIH HHS · GM27241 · United States
Databases
GENBANK
M22752
External Links
PubMed source DOI: 10.1128/jb.171.1.473-482.1989 PMC Full Text View journal details
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