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PMID: 2495790 Published · ppublish English Journal Article Research Support, Non-U.S. Gov't

Feedback control of ornithine decarboxylase expression by polyamines. Analysis of ornithine decarboxylase mRNA distribution in polysome profiles and of translation of this mRNA in vitro.

The Biochemical journal ·Vol. 258 ·No. 2 ·1989-03-01 ·Pages 343-50

Holm I, Persson L, Stjernborg L, Thorsson L, Heby O

Abstract

Cell growth and differentiation require the presence of optimal concentrations of polyamines. Ornithine decarboxylase (ODC) catalyses the first and rate-controlling step in polyamine synthesis. In studies using cultures of Ehrlich ascites-tumour cells, we have shown that the expression of ODC is subject to feedback regulation by the polyamines. A decrease in the cellular polyamine concentration results in a compensatory increase in the synthesis of ODC, whereas an increase in polyamine concentration results in suppression of ODC synthesis. These changes in ODC synthesis were attributed to changes in the efficiency of ODC mRNA translation, because the steady-state amount of ODC mRNA remained constant. We now show that the number of ribosomes associated with ODC mRNA is low, and that the increase in ODC mRNA translation takes place without a shift in the distribution of ODC mRNA towards larger polysomes. This finding indicates that the polyamines regulate the efficiency of ODC mRNA translation by co-ordinately affecting the rates of initiation and elongation. By analysing ODC mRNA translation in vitro, using a rabbit reticulocyte lysate, polyadenylated RNA from a cell line with an amplified ODC gene, and a monospecific anti-ODC antibody, we also show that spermidine, but not putrescine, exerts a direct regulatory effect on ODC synthesis.

MeSH Terms
Animals Eflornithine/pharmacology Feedback Ornithine Decarboxylase/biosynthesis Polyamines/metabolism Polyribosomes/analysis Protein Biosynthesis RNA, Messenger/analysis Rabbits Reticulocytes/metabolism Tumor Cells, Cultured/drug effects
Chemicals
Polyamines RNA, Messenger Ornithine Decarboxylase Eflornithine
Authors & Affiliations
5 authors, click to expand affiliations / ORCID
Holm I
Department of Zoophysiology, University of Lund, Sweden.
Persson L
Stjernborg L
Thorsson L
Heby O
References (39)
39 references, click to expand
  1. Amine synthesis in regenerating rat liver: extremely rapid turnover of ornithine decarboxylase.
    Mol Pharmacol. 1969 May;5(3):253-62 PMID: 5783961
  2. Inhibition of translation of mRNAs for ornithine decarboxylase and S-adenosylmethionine decarboxylase by polyamines.
    J Biol Chem. 1987 Feb 25;262(6):2427-30 PMID: 3818602
  3. Control of ornithine decarboxylase activity in stimulated human lymphocytes by putrescine and spermidine.
    Biochem J. 1973 Apr;132(4):791-6 PMID: 4721611
  4. Effect of growth conditions on the activity of ornithine decarboxylase in cultured hepatoma cells. I. Effect of amino acid supply.
    J Cell Physiol. 1974 Jun;83(3):345-51 PMID: 4363880
  5. Ribonucleic acid isolated by cesium chloride centrifugation.
    Biochemistry. 1974 Jun 4;13(12):2633-7 PMID: 4831907
  6. Specific induction of ornithine decarboxylase in 3T3 mouse fibroblasts by pituitary growth factors: cell density-dependent biphasic response and alteration of half-life.
    Biochemistry. 1974 Oct 22;13(22):4668-74 PMID: 4473203
  7. Regulation of ornithine decarboxylase activity by putrescine and spermidine in rat liver.
    Biochem Biophys Res Commun. 1974 Nov 27;61(2):449-56 PMID: 4156172
  8. Effect of DL-alpha-hydrazino-delta-aminovaleric acid, an inhibitor of ornithine decarboxylase, on polyamine metabolism in isoproterenol-stimulated mouse parotid glands.
    J Biochem. 1975 Apr;77(4):879-93 PMID: 1150642
  9. Polyamine transport and metabolism in mouse mammary gland. General properties and hormonal regulation.
    J Biol Chem. 1976 May 10;251(9):2795-800 PMID: 1262344
  10. Effect of alpha-methyl ornithine on ornithine decarboxylase activity of rat hepatoma cells in culture.
    Biochem Biophys Res Commun. 1977 Jun 6;76(3):893-9 PMID: 197931
  11. Initial characterization of a HTC cell variant partially resistant to the anti-proliferative effect of ornithine decarboxylase inhibitors.
    Exp Cell Res. 1978 Sep;115(2):387-93 PMID: 689093
  12. Irreversible inhibition of the early increase in ornithine decarboxylase activity following growth stimulation is required to block Ehrlich ascites tumor cell proliferation in culture.
    Biochem Biophys Res Commun. 1980 May 14;94(1):151-8 PMID: 6770860
  13. Decarboxylation of ornithine and lysine by ornithine decarboxylase from kidneys of testosterone treated mice.
    Acta Chem Scand B. 1981;35(6):451-9 PMID: 6797167
  14. Role of polyamines in the control of cell proliferation and differentiation.
    Differentiation. 1981;19(1):1-20 PMID: 6173280
  15. Purification and some properties of ornithine decarboxylase from rat liver.
    Biochim Biophys Acta. 1982 Jul 16;717(1):111-7 PMID: 6809059
  16. Changes in mouse kidney ornithine decarboxylase activity are brought about by changes in the amount of enzyme protein as measured by radioimmunoassay.
    J Biol Chem. 1983 Feb 25;258(4):2496-500 PMID: 6401734
  17. Transcriptional activation of mammalian ornithine decarboxylase during stimulated growth.
    Mol Cell Biol. 1987 Jul;7(7):2641-3 PMID: 3614203
  18. The induction of ornithine decarboxylase by the tumor promoter TPA is controlled at the post-transcriptional level in murine Swiss 3T3 fibroblasts.
    Biochem Biophys Res Commun. 1987 Aug 31;147(1):219-25 PMID: 3632665
  19. Regulation of ornithine decarboxylase mRNA translation by polyamines. Studies using a cell-free system and a cell line with an amplified ornithine decarboxylase gene.
    J Biol Chem. 1988 Mar 5;263(7):3528-33 PMID: 3125182
  20. Isolation and characterization of the mouse ornithine decarboxylase gene.
    J Biol Chem. 1988 Jun 5;263(16):7604-9 PMID: 3372502
  21. Antibodies to ornithine decarboxylase. Immunochemical cross-reactivity.
    Acta Chem Scand B. 1982;36(10):685-8 PMID: 7164704
  22. The preparation and properties of gel-filtered rabbit-reticulocyte lysate protein-synthesis systems.
    Eur J Biochem. 1983 Mar 15;131(2):289-301 PMID: 6832153
  23. DNA sequence polymorphism in an androgen-regulated gene is associated with alteration in the encoded RNAs.
    Proc Natl Acad Sci U S A. 1983 Jan;80(2):501-4 PMID: 6188152
  24. Ornithine decarboxylase in mouse kidney. Purification, characterization, and radioimmunological determination of the enzyme protein.
    J Biol Chem. 1983 Jun 10;258(11):6735-40 PMID: 6853503
  25. A technique for radiolabeling DNA restriction endonuclease fragments to high specific activity.
    Anal Biochem. 1983 Jul 1;132(1):6-13 PMID: 6312838
  26. A mouse lymphoma cell mutant whose major protein product is ornithine decarboxylase.
    J Biol Chem. 1983 Oct 25;258(20):12083-6 PMID: 6415048
  27. Molecular cloning and expression of the mouse ornithine decarboxylase gene.
    Proc Natl Acad Sci U S A. 1984 Jan;81(2):540-4 PMID: 6582509
  28. Androgen induction of ornithine decarboxylase mRNA in mouse kidney as studied by complementary DNA.
    Proc Natl Acad Sci U S A. 1984 Feb;81(3):731-5 PMID: 6366788
  29. Isolation of cloned cDNA encoding mammalian ornithine decarboxylase.
    Proc Natl Acad Sci U S A. 1984 Jun;81(12):3645-9 PMID: 6587379
  30. Androgen-regulated ornithine decarboxylase mRNAs of mouse kidney.
    J Biol Chem. 1984 Jun 25;259(12):7941-6 PMID: 6330090
  31. Polyamines.
    Annu Rev Biochem. 1984;53:749-90 PMID: 6206782
  32. Investigation of structure and rate of synthesis of ornithine decarboxylase protein in mouse kidney.
    Biochemistry. 1984 Jul 31;23(16):3777-83 PMID: 6477895
  33. Mouse ornithine decarboxylase. Complete amino acid sequence deduced from cDNA.
    J Biol Chem. 1985 Mar 10;260(5):2941-4 PMID: 2982844
  34. Ornithine decarboxylase inhibitors increase the cellular content of the enzyme: implications for translational regulation.
    Biochem Biophys Res Commun. 1985 Aug 30;131(1):239-45 PMID: 3929778
  35. Translational regulation of mammalian ornithine decarboxylase by polyamines.
    J Biol Chem. 1985 Dec 15;260(29):15390-3 PMID: 3934157
  36. Recent advances in the biochemistry of polyamines in eukaryotes.
    Biochem J. 1986 Mar 1;234(2):249-62 PMID: 3087344
  37. Control of ornithine decarboxylase in Chinese hamster ovary cells by polyamines. Translational inhibition of synthesis and acceleration of degradation of the enzyme by putrescine, spermidine, and spermine.
    J Biol Chem. 1986 Jul 15;261(20):9502-8 PMID: 3722208
  38. Translational regulation of ornithine decarboxylase by polyamines.
    FEBS Lett. 1986 Sep 15;205(2):175-8 PMID: 3743773
  39. Dissociation of putrescine-activated decarboxylation of S-adenosyl-L-methionine from the enzymic synthesis of spermidine and spermine by purified prostatic enzyme preparations.
    Biochem Biophys Res Commun. 1971 Jan 22;42(2):222-9 PMID: 5545514
Article Info
Journal
The Biochemical journal
Abbr.
Biochem J
ISSN
0264-6021
DOI
10.1042/bj2580343
Published
1989-03-01
Pages
343-50
Language
English
Region
England
NLM ID
2984726R
PMCID
PMC1138367
Subset
IM
External Links
PubMed source DOI: 10.1042/bj2580343 PMC Full Text View journal details
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