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PMID: 2497227 Published · ppublish English Journal Article

Murine V kappa gene expression does not follow the VH paradigm.

The Journal of experimental medicine ·Vol. 169 ·No. 5 ·1989-05-01 ·Pages 1859-64

Kaushik A, Schulze DH, Bona C, Kelsoe G

Abstract

V kappa gene family expression among LPS-reactive murine B lymphocytes, unlike that of VH gene families, is not proportional to genomic complexity, i.e., nonstoichiometric. Furthermore, no positional bias for the overexpression of J-proximal V kappa genes (V kappa 21) is observed among neonatal B lymphocytes. Yet, the V kappa 1 and V kappa 9 families located in the center of V kappa locus are preferentially used by neonatal B splenocytes. Thus, the mechanisms of V kappa gene rearrangement and expression appear to differ significantly from those controlling the VH locus.

MeSH Terms
Animals Animals, Newborn DNA Probes Gene Expression Regulation Genes, Immunoglobulin Immunoglobulin Heavy Chains/genetics Immunoglobulin Variable Region/genetics Immunoglobulin kappa-Chains/genetics Mice Mice, Inbred C57BL Nucleic Acid Hybridization
Chemicals
DNA Probes Immunoglobulin Heavy Chains Immunoglobulin Variable Region Immunoglobulin kappa-Chains
Authors & Affiliations
4 authors, click to expand affiliations / ORCID
Kaushik A
Department of Microbiology, Mount Sinai School of Medicine, New York 10029.
Schulze D H
Bona C
Kelsoe G
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22 references, click to expand
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Article Info
Journal
The Journal of experimental medicine
Abbr.
J Exp Med
ISSN
0022-1007
DOI
10.1084/jem.169.5.1859
Published
1989-05-01
Pages
1859-64
Language
English
Region
United States
NLM ID
2985109R
PMCID
PMC2189305
Subset
IM
External Links
PubMed source DOI: 10.1084/jem.169.5.1859 PMC Full Text View journal details
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