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PMID: 2502538 Published · ppublish English Journal Article

Induction of ermC methylase in the absence of macrolide antibiotics and by pseudomonic acid A.

Journal of bacteriology ·Vol. 171 ·No. 8 ·1989-08-00 ·Pages 4518-20

Kadam SK

Abstract

The methylase encoded by erm genes and induced by erythromycin modifies the 23S rRNA and confers resistance to macrolide-lincosamide-streptogramin B antibiotics. Induction is due to a posttranscriptional mechanism in which the inducer activates translation of methylase mRNA by binding to unmethylated (erythromycin-sensitive) ribosomes and stalling them in the leader region. It is shown in this study that pseudomonic acid A, an inhibitor of isoleucyl-tRNA synthetase, can also induce methylase synthesis. Isoleucine starvation has a similar effect on ribosomes translating the ermC leader region to cause induction of methylase synthesis. These observations support the requirements for ribosome stalling and destabilization of a stem-loop structure and demonstrate that stalling can occur without macrolide-bound ribosomes.

MeSH Terms
Anti-Bacterial Agents/pharmacology Bacillus subtilis/drug effects,enzymology,genetics Enzyme Induction Erythromycin/pharmacology Fatty Acids/pharmacology Kinetics Methyltransferases/biosynthesis,genetics Mupirocin Plasmids beta-Galactosidase/biosynthesis
Chemicals
Anti-Bacterial Agents Fatty Acids Erythromycin Mupirocin Methyltransferases rRNA (adenosine-O-2'-)methyltransferase beta-Galactosidase
Authors & Affiliations
1 authors, click to expand affiliations / ORCID
Kadam S K
Anti Infective Research Division, Abbott Laboratories, Abbott Park, Illinois 60064.
References (19)
19 references, click to expand
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Article Info
Journal
Journal of bacteriology
Abbr.
J Bacteriol
ISSN
0021-9193
DOI
10.1128/jb.171.8.4518-4520.1989
Published
1989-08-00
Pages
4518-20
Language
English
Region
United States
NLM ID
2985120R
PMCID
PMC210237
Subset
IM
External Links
PubMed source DOI: 10.1128/jb.171.8.4518-4520.1989 PMC Full Text View journal details
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