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PMID: 6159624 Published · ppublish English Journal Article Research Support, U.S. Gov't, Non-P.H.S. Research Support, U.S. Gov't, P.H.S.

Posttranscriptional regulation of an erythromycin resistance protein specified by plasmic pE194.

Proceedings of the National Academy of Sciences of the United States of America ·Vol. 77 ·No. 7 ·1980-07-00 ·Pages 3903-7

Shivakumar AG, Hahn J, Grandi G, Kozlov Y, Dubnau D

Abstract

Induction of the synthesis of a plasmid-encoded polypeptide (E3) by erythromycin is known to be required for the inducible expression of resistance to the macrolide-lincosamide-streptogramin B group of antibiotics in Bacillus subtilis strains carrying the plasmid pE194. This resistance is mediated by a specific N6-dimethylation of adenine in the 23S rRNA of the large ribosomal subunit. We show in this report that E3 induction is regulated posttranscriptionally in the sense that it can occur when RNA synthesis is blocked and that induction is accompanied by an increase in the functional half-life of E3 mRNA but not of the mRNA species that code for the remaining four known pE194 polypeptides. The induction of E3 is subject to feedback regulation and involves the ribosome. Modification of the erythromycin binding site on the ribosome by methylation or by mutation interferes with induction.

MeSH Terms
Bacillus subtilis/genetics Drug Resistance, Microbial Erythromycin Feedback Gene Expression Regulation Plasmids RNA, Bacterial/genetics RNA, Messenger/genetics Ribosomes/metabolism Transcription, Genetic
Chemicals
RNA, Bacterial RNA, Messenger Erythromycin
Authors & Affiliations
5 authors, click to expand affiliations / ORCID
Shivakumar A G
Hahn J
Grandi G
Kozlov Y
Dubnau D
References (28)
28 references, click to expand
  1. REQUIREMENTS FOR TRANSFORMATION IN BACILLUS SUBTILIS.
    J Bacteriol. 1961 May;81(5):741-6 PMID: 16561900
  2. Induction of erythromycin resistance in Staphyloccus aureus by erythromycin derivatives.
    Antimicrob Agents Chemother. 1976 Jan;9(1):128-30 PMID: 1259386
  3. Erythromycin-inducible resistance in Staphylococcus aureus: requirements for induction.
    J Bacteriol. 1971 Jun;106(3):835-47 PMID: 4397638
  4. Macrolide resistance in Staphylococcus aureus: inducers of macrolide resistance.
    Antimicrob Agents Chemother. 1977 Apr;11(4):669-74 PMID: 856018
  5. Drug resistance in Staphylococcus aureus. Induction of macrolide resistance by erythromycin, oleandomycin and their derivatives.
    Jpn J Microbiol. 1975 Oct;19(5):343-7 PMID: 1223337
  6. Characterization of plasmid transformation in Bacillus subtilis: kinetic properties and the effect of DNA conformation.
    Mol Gen Genet. 1979 Jan 2;167(3):251-8 PMID: 105246
  7. Binding of [14C]erythromycin to Escherichia coli ribosomes.
    Antimicrob Agents Chemother. 1974 Oct;6(4):474-8 PMID: 4157348
  8. Nucleotide sequence at the binding site for coat protein on RNA of bacteriophage R17.
    Proc Natl Acad Sci U S A. 1972 Oct;69(10):3033-7 PMID: 4507620
  9. Three distinct plasmids originating in the same Staphylococcus aureus strain.
    Arch Roum Pathol Exp Microbiol. 1976 Jan-Jun;35(1-2):111-8 PMID: 1008685
  10. Plasmid copy number control: isolation and characterization of high-copy-number mutants of plasmid pE194.
    J Bacteriol. 1979 Jan;137(1):635-43 PMID: 104975
  11. Protein synthesis in Bacillus subtilis. II. Selective translation of natural mRNAs and its possible relation to the species-specific inhibition of protein synthesis by lincomycin and erythromycin.
    J Mol Biol. 1979 Dec 15;135(3):627-38 PMID: 94104
  12. Drug resistance of staphylococci. Foation of erythromycin-ribosome complex. Decrease in the formation of erythromycin-ribosome complex in erythromycin resistant strains of Staphylococcus aureus.
    Jpn J Microbiol. 1969 Mar;13(1):119-21 PMID: 5305691
  13. Studies on the synthesis of plasmid-coded proteins and their control in Bacillus subtilis minicells.
    Plasmid. 1979 Apr;2(2):279-89 PMID: 109873
  14. Construction and properties of chimeric plasmids in Bacillus subtilis.
    Proc Natl Acad Sci U S A. 1978 Mar;75(3):1428-32 PMID: 418412
  15. Transcriptional and post-transcriptional control of RNA polymerase and ribosomal protein genes cloned on composite ColE1 plasmids in the bacterium Escherichia coli.
    J Biol Chem. 1979 Aug 25;254(16):7540-7 PMID: 381287
  16. A quantitative assay for DNA-RNA hybrids with DNA immobilized on a membrane.
    J Mol Biol. 1965 Jul;12(3):829-42 PMID: 4955314
  17. Use of antibiotics to determine ribosome-messenger RNA interactions necessary for in vivo stability of specific messengers.
    J Mol Biol. 1977 Aug 25;114(4):569-73 PMID: 915943
  18. Altered methylation of ribosomal RNA in an erythromycin-resistant strain of Staphylococcus aureus.
    Proc Natl Acad Sci U S A. 1971 Apr;68(4):856-60 PMID: 5279527
  19. Effects of macrolide antibiotics on the ribosomal peptidyl transferase in cell-free systems derived from Escherichia coli B and erythromycin-resistant muytant of Escherichia coli B.
    Biochim Biophys Acta. 1971 Jun 17;240(1):109-21 PMID: 4940152
  20. Minicells of Bacillus subtilis.
    J Bacteriol. 1973 May;114(2):860-73 PMID: 4196259
  21. Erythromycin resistant mutations in Bacillus subtilis cause temperature sensitive sporulation.
    Mol Gen Genet. 1977 Jan 18;150(2):147-59 PMID: 402547
  22. Conditional mutations in the translational apparatus of Bacillus subtils.
    Mol Gen Genet. 1976 Aug 10;147(1):1-12 PMID: 822278
  23. Antibiotics as probes of ribosome structure: binding of chloramphenicol and erythromycin to polyribosomes; effect of other antibiotics.
    Antimicrob Agents Chemother. 1974 Mar;5(3):255-67 PMID: 4599122
  24. Regulation of ribosomal protein synthesis in Escherichia coli by selective mRNA inactivation.
    Proc Natl Acad Sci U S A. 1979 Jul;76(7):3411-5 PMID: 158759
  25. Autogenous translational repression of bacteriophage T4 gene 32 expression in vitro.
    J Mol Biol. 1978 Nov 25;126(1):73-90 PMID: 739544
  26. Hyper-labile messenger RNA in polar mutants of the tryptophan operon of Escherichia coli.
    J Mol Biol. 1972 Dec 14;72(1):103-10 PMID: 4567396
  27. Structure of an inducibly methylatable nucleotide sequence in 23S ribosomal ribonucleic acid from erythromycin-resistant Staphylococcus aureus.
    Biochemistry. 1973 Jan 30;12(3):457-60 PMID: 4683489
  28. Alteration of 23 S ribosomal RNA and erythromycin-induced resistance to lincomycin and spiramycin in Staphylococcus aureus.
    J Mol Biol. 1973 Feb 15;74(1):67-72 PMID: 4731016
Article Info
Journal
Proceedings of the National Academy of Sciences of the United States of America
Abbr.
Proc Natl Acad Sci U S A
ISSN
0027-8424
DOI
10.1073/pnas.77.7.3903
Published
1980-07-00
Pages
3903-7
Language
English
Region
United States
NLM ID
7505876
PMCID
PMC349735
Subset
IM
Grants
NIAID NIH HHS · AI-10311 · United States
External Links
PubMed source DOI: 10.1073/pnas.77.7.3903 PMC Full Text View journal details
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