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PMID: 2503709 Published · ppublish English Journal Article Research Support, Non-U.S. Gov't

Prosomes and heat shock complexes in Drosophila melanogaster cells.

Molecular and cellular biology ·Vol. 9 ·No. 6 ·1989-06-00 ·Pages 2672-81

de Sa CM, Rollet E, de Sa MF, Tanguay RM, Best-Belpomme M, Scherrer K

Abstract

Prosomes and heat shock protein (HSP) complexes isolated from the cytoplasm of Drosophila cells in culture were biochemically and immunologically characterized. The two complexes were found to separate on sucrose gradients, allowing the analysis of their protein constituents by two-dimensional polyacrylamide gel electrophoresis and by reaction with anti-HSP sera and prosome-specific monoclonal antibodies. All of the prosomal proteins were found to be clearly distinct from the HSP; none of the prosomal proteins was synthesized de novo in heat shock. However, an antiprosome (anti-p27K) monoclonal antibody (mouse anti-duck) recognizing the Drosophila p29K prosomal protein allowed immunoprecipitation from a heat-shocked postmitochondrial supernatant of the crude HSP complex, including the low- and the high-molecular-weight components, in particular the 70 x 10(3)-molecular weight HSP. The highly purified small 16S HSP complex still contained this preexistent p29K prosomal protein, which thus also seems to be a metabolically stable constituent of the HSP complex. The significance of this structural and possibly functional relationship between prosomes and HSP, involving the highly ubiquitous and evolutionarily conserved prosomal protein p27/29K, remains to be elucidated.

MeSH Terms
Animals Antibodies, Monoclonal Biological Evolution Cell Fractionation Cell Nucleus/analysis Cells, Cultured Centrifugation, Density Gradient Cytoplasm/analysis Drosophila melanogaster/analysis,cytology Electrophoresis, Polyacrylamide Gel Heat-Shock Proteins/analysis Immunoblotting Molecular Weight Peptide Mapping Precipitin Tests Ribonucleoproteins/analysis
Chemicals
Antibodies, Monoclonal Heat-Shock Proteins Ribonucleoproteins
Authors & Affiliations
6 authors, click to expand affiliations / ORCID
de Sa C M
Institut Jacques Monod, Paris, France.
Rollet E
de Sa M F
Tanguay R M
Best-Belpomme M
Scherrer K
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Article Info
Journal
Molecular and cellular biology
Abbr.
Mol Cell Biol
ISSN
0270-7306
DOI
10.1128/mcb.9.6.2672-2681.1989
Published
1989-06-00
Pages
2672-81
Language
English
Region
United States
NLM ID
8109087
PMCID
PMC362340
Subset
IM
External Links
PubMed source DOI: 10.1128/mcb.9.6.2672-2681.1989 PMC Full Text View journal details
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