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PMID: 25043187 Published · ppublish English Journal Article Research Support, N.I.H., Extramural Research Support, Non-U.S. Gov't

A genome-scale resource for the functional characterization of Arabidopsis transcription factors.

Cell reports ·Vol. 8 ·No. 2 ·2014-07-24 ·Pages 622-32

Pruneda-Paz JL, Breton G, Nagel DH, Kang SE, Bonaldi K, Doherty CJ, Ravelo S, Galli M, Ecker JR, Kay SA

Abstract

Extensive transcriptional networks play major roles in cellular and organismal functions. Transcript levels are in part determined by the combinatorial and overlapping functions of multiple transcription factors (TFs) bound to gene promoters. Thus, TF-promoter interactions provide the basic molecular wiring of transcriptional regulatory networks. In plants, discovery of the functional roles of TFs is limited by an increased complexity of network circuitry due to a significant expansion of TF families. Here, we present the construction of a comprehensive collection of Arabidopsis TFs clones created to provide a versatile resource for uncovering TF biological functions. We leveraged this collection by implementing a high-throughput DNA binding assay and identified direct regulators of a key clock gene (CCA1) that provide molecular links between different signaling modules and the circadian clock. The resources introduced in this work will significantly contribute to a better understanding of the transcriptional regulatory landscape of plant genomes.

MeSH Terms
Arabidopsis/genetics Arabidopsis Proteins/genetics Gene Expression Regulation, Plant Genome, Plant Transcription Factors/genetics
Chemicals
Arabidopsis Proteins CCA1 protein, Arabidopsis Transcription Factors
Authors & Affiliations
10 authors, click to expand affiliations / ORCID
Pruneda-Paz Jose L
Division of Biological Sciences, University of California, San Diego, La Jolla, CA 92093, USA; Center for Chronobiology, University of California, San Diego, La Jolla, CA 92093, USA. Electronic address: [email protected].
Breton Ghislain
Division of Biological Sciences, University of California, San Diego, La Jolla, CA 92093, USA.
Nagel Dawn H
Division of Biological Sciences, University of California, San Diego, La Jolla, CA 92093, USA; Center for Chronobiology, University of California, San Diego, La Jolla, CA 92093, USA.
Kang S Earl
Division of Biological Sciences, University of California, San Diego, La Jolla, CA 92093, USA; Center for Chronobiology, University of California, San Diego, La Jolla, CA 92093, USA.
Bonaldi Katia
Division of Biological Sciences, University of California, San Diego, La Jolla, CA 92093, USA; Center for Chronobiology, University of California, San Diego, La Jolla, CA 92093, USA.
Doherty Colleen J
Division of Biological Sciences, University of California, San Diego, La Jolla, CA 92093, USA; Center for Chronobiology, University of California, San Diego, La Jolla, CA 92093, USA.
Ravelo Stephanie
Division of Biological Sciences, University of California, San Diego, La Jolla, CA 92093, USA.
Galli Mary
Genomic Analysis Laboratory, Howard Hughes Medical Institute and The Salk Institute for Biological Studies, La Jolla, CA 92037, USA.
Ecker Joseph R
Genomic Analysis Laboratory, Howard Hughes Medical Institute and The Salk Institute for Biological Studies, La Jolla, CA 92037, USA.
Kay Steve A
Division of Biological Sciences, University of California, San Diego, La Jolla, CA 92093, USA; Center for Chronobiology, University of California, San Diego, La Jolla, CA 92093, USA. Electronic address: [email protected].
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Article Info
Journal
Cell reports
Abbr.
Cell Rep
ISSN
2211-1247
DOI
10.1016/j.celrep.2014.06.033
Email
[email protected]
[email protected]
Published
2014-07-24
Epub
2014-00-17
Pages
622-32
Language
English
Region
United States
NLM ID
101573691
PMCID
PMC4125603
Subset
IM
Grants
NIGMS NIH HHS · R01 GM067837 · United States
NIGMS NIH HHS · RC2GM092412 · United States
Howard Hughes Medical Institute · United States
NIGMS NIH HHS · R01 GM056006 · United States
NIGMS NIH HHS · R01GM056006 · United States
NIGMS NIH HHS · R01GM067837 · United States
NIGMS NIH HHS · RC2 GM092412 · United States
External Links
PubMed source DOI: 10.1016/j.celrep.2014.06.033 PMC Full Text View journal details
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