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PMID: 2511419 Published · ppublish English Journal Article Research Support, Non-U.S. Gov't

Molecular cloning and nucleotide sequencing of oxyR, the positive regulatory gene of a regulon for an adaptive response to oxidative stress in Escherichia coli: homologies between OxyR protein and a family of bacterial activator proteins.

Molecular & general genetics : MGG ·Vol. 218 ·No. 3 ·1989-09-00 ·Pages 371-6

Tao K, Makino K, Yonei S, Nakata A, Shinagawa H

Abstract

Treatment of Escherichia coli and Salmonella typhimurium cells with a low dose of hydrogen peroxide induces expression of a large number of genes, and confers resistance to oxidative stresses. The oxyR gene encodes a positive regulatory protein for a subset of these genes involved in the defense against oxidative damage. We cloned a DNA fragment that contains the E. coli oxyR region on a plasmid vector, and analyzed the nucleotide sequence of the gene. The amino acid sequence of OxyR protein, deduced from the nucleotide sequence, shows a high degree of homology to the sequences of a number of bacterial activator proteins including LysR, CysB, IlvY, MetR and NodD. The product of the oxyR gene identified by the maxicell procedure was a 34 kDa protein, which agrees with the size predicted from the nucleotide sequence of the gene.

MeSH Terms

Amino Acid Sequence Bacterial Proteins/genetics Base Sequence Cloning, Molecular DNA-Binding Proteins Electrophoresis, Polyacrylamide Gel Escherichia coli/genetics Escherichia coli Proteins Genes, Regulator Hydrogen Peroxide/pharmacology Molecular Sequence Data Oxygen/metabolism Plasmids Repressor Proteins Restriction Mapping Sequence Homology, Nucleic Acid Transcription Factors beta-Galactosidase

Chemicals

Bacterial Proteins DNA-Binding Proteins Escherichia coli Proteins Repressor Proteins Transcription Factors oxyR protein, E coli Hydrogen Peroxide beta-Galactosidase Oxygen

Authors & Affiliations

5 authors, click to expand affiliations / ORCID

Tao K

Laboratory of Radiation Biology, Faculty of Science, Kyoto University, Japan.

Makino K

Yonei S

Nakata A

Shinagawa H

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Article Info

Journal

Molecular & general genetics : MGG

Abbr.

Mol Gen Genet

ISSN

0026-8925

DOI

10.1007/BF00332397

Published

1989-09-00

Pages

371-6

Language

English

Region

Germany

NLM ID

0125036

Subset

External Links

PubMed source DOI: 10.1007/BF00332397

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Molecular cloning and nucleotide sequencing of oxyR, the positive regulatory gene of a regulon for an adaptive response to oxidative stress in Escherichia coli: homologies between OxyR protein and a family of bacterial activator proteins.

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