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PMID: 2514622 Published · ppublish English Journal Article Research Support, Non-U.S. Gov't

BRO beta-lactamases of Branhamella catarrhalis and Moraxella subgenus Moraxella, including evidence for chromosomal beta-lactamase transfer by conjugation in B. catarrhalis, M. nonliquefaciens, and M. lacunata.

Antimicrobial agents and chemotherapy ·Vol. 33 ·No. 11 ·1989-11-00 ·Pages 1845-54

Wallace RJ, Steingrube VA, Nash DR, Hollis DG, Flanagan C, Brown BA, Labidi A, Weaver RE

Abstract

Two closely related beta-lactamases, BRO-1 and BRO-2 (formerly called Ravasio and 1908), are found in Moraxella (Branhamella) catarrhalis. We screened strains of B. catarrhalis recovered in the United States since 1952 and identified the first beta-lactamase-positive isolate in August 1976. The prevalence of the enzymes among 394 clinical isolates from one Texas hospital has averaged 75% since testing began in 1983. Screening of isolates of Moraxella subgenus Moraxella revealed the BRO enzymes in two other human respiratory tract species, M. lacunata and M. nonliquefaciens, beginning in 1978. A different beta-lactamase with a pI of 6.4 predominated in other species of subgenus Moraxella. BRO-2 had a different isoelectric focusing pattern and was produced in lesser amounts than BRO-1, but the two enzymes were indistinguishable by substrate or inhibitor profile. BRO enzymes from B. catarrhalis, M. nonliquefaciens, and M. lacunata could be transferred by conjugation and, for B. catarrhalis, also by transformation to B. catarrhalis. Plasmid bands were demonstrated in 90% of M. nonliquefaciens and in one previously reported strain of B. catarrhalis, but no change in plasmid profiles was seen in beta-lactamase-positive recombinants, supporting previous studies that suggested the beta-lactamase genes are chromosomal.

MeSH Terms
Chromosomes, Bacterial Conjugation, Genetic DNA, Bacterial/genetics Kinetics Moraxella/enzymology,genetics Moraxella catarrhalis/enzymology,genetics Plasmids Substrate Specificity Transformation, Bacterial beta-Lactamase Inhibitors beta-Lactamases/metabolism
Chemicals
DNA, Bacterial beta-Lactamase Inhibitors beta-Lactamases
Authors & Affiliations
8 authors, click to expand affiliations / ORCID
Wallace R J
Department of Microbiology, University of Texas Health Center, Tyler 75710.
Steingrube V A
Nash D R
Hollis D G
Flanagan C
Brown B A
Labidi A
Weaver R E
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Article Info
Journal
Antimicrobial agents and chemotherapy
Abbr.
Antimicrob Agents Chemother
ISSN
0066-4804
DOI
10.1128/AAC.33.11.1845
Published
1989-11-00
Pages
1845-54
Language
English
Region
United States
NLM ID
0315061
PMCID
PMC172775
Subset
IM
External Links
PubMed source DOI: 10.1128/AAC.33.11.1845 PMC Full Text View journal details
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