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PMID: 2521618 Published · ppublish English Journal Article Research Support, Non-U.S. Gov't Research Support, U.S. Gov't, P.H.S.

Retroregulation of the bacteriophage lambda int gene: limited secondary degradation of the RNase III-processed transcript.

Journal of bacteriology ·Vol. 171 ·No. 1 ·1989-01-00 ·Pages 588-92

Plunkett G, Echols H

Abstract

Expression of the int gene of bacteriophage lambda from two promoters, pI and pL, is differentially regulated through RNA processing. Efficient Int protein synthesis from the pL RNA is inhibited by the action of sib, a cis-acting retroregulator downstream from the int gene. We have used mapping procedures with nuclease S1 to study the pL transcripts produced in vivo after phage lambda infection. We have found an RNase III-dependent processing site within the Int coding sequence, 387 nucleotides upstream from the site of the primary cleavage by RNase III at Sib. This secondary processing site is located at the most stable region of secondary structure in the sib int region, as predicted by computer analysis. We suggest that RNase III cleavage at the Sib site allows processive exonucleolytic degradation of the RNA to proceed to a region of secondary structure within the Int coding sequence, which protects the upstream region of the transcript from further degradation.

MeSH Terms
Bacteriophage lambda/enzymology,genetics Base Sequence DNA Nucleotidyltransferases/genetics Endoribonucleases/metabolism Escherichia coli/genetics Escherichia coli Proteins Gene Expression Regulation Genes Genes, Viral Integrases Molecular Sequence Data Nucleic Acid Conformation Promoter Regions, Genetic RNA, Messenger/genetics Ribonuclease III Transcription, Genetic
Chemicals
Escherichia coli Proteins RNA, Messenger DNA Nucleotidyltransferases Integrases Endoribonucleases Ribonuclease III ribonuclease III, E coli
Authors & Affiliations
2 authors, click to expand affiliations / ORCID
Plunkett G
Department of Molecular Biology, University of California, Berkeley 94720.
Echols H
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Article Info
Journal
Journal of bacteriology
Abbr.
J Bacteriol
ISSN
0021-9193
DOI
10.1128/jb.171.1.588-592.1989
Published
1989-01-00
Pages
588-92
Language
English
Region
United States
NLM ID
2985120R
PMCID
PMC209629
Subset
IM
Grants
NIGMS NIH HHS · GM 17078 · United States
External Links
PubMed source DOI: 10.1128/jb.171.1.588-592.1989 PMC Full Text View journal details
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