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PMID: 2523868 Published · ppublish English Comparative Study Journal Article Research Support, Non-U.S. Gov't

The extra segments of sequence in rat leucocyte common antigen (L-CA) are derived by alternative splicing of only three exons and show extensive O-linked glycosylation.

Immunogenetics ·Vol. 29 ·No. 5 ·1989-00-00 ·Pages 281-7

Jackson DI, Barclay AN

Abstract

The leucocyte common antigen (L-CA, CD45, or T200) consists of a family of heavily glycosylated glycoproteins of apparent Mr 180,000-240,000 found at the surface of leucocytes but not other cell types. Populations of lymphocytes express forms that differ in antigenicity, apparent Mr, and glycosylation. Some of this heterogeneity is due to polypeptide differences caused by the insertion of up to three different segments of sequence near the NH2-terminus. We report the complete sequence of the region of the rat L-CA gene encoding the extra segments. Analysis of this sequence showed that each segment was encoded by an exon but no further exons could be identified, implying that the polypeptide heterogeneity is solely due to selection from these three exons by alternative splicing. Amino acid sequencing of glycopeptides prepared from the largest forms of L-CA indicated extensive O-linked glycosylation in at least one of the extra segments.

MeSH Terms
Amino Acid Sequence Animals Antigens, Differentiation/genetics Base Sequence Exons Glycosylation Histocompatibility Antigens/genetics Humans Leukocyte Common Antigens Mice Molecular Sequence Data Molecular Weight Protein Processing, Post-Translational Protein Sorting Signals RNA Splicing Rats Rats, Inbred Strains
Chemicals
Antigens, Differentiation Histocompatibility Antigens Protein Sorting Signals Leukocyte Common Antigens
Authors & Affiliations
2 authors, click to expand affiliations / ORCID
Jackson D I
MRC Cellular Immunology Unit, Sir William Dunn School of Pathology, University of Oxford, United Kingdom.
Barclay A N
References (39)
39 references, click to expand
  1. A rapid single-stranded cloning strategy for producing a sequential series of overlapping clones for use in DNA sequencing: application to sequencing the corn mitochondrial 18 S rDNA.
    Plasmid. 1985 Jan;13(1):31-40 PMID: 3991809
  2. T cells that help B cell responses to soluble antigen are distinguishable from those producing interleukin 2 on mitogenic or allogeneic stimulation.
    J Exp Med. 1986 Apr 1;163(4):774-86 PMID: 2936864
  3. Differential usage of three exons generates at least five different mRNAs encoding human leukocyte common antigens.
    J Exp Med. 1987 Nov 1;166(5):1548-66 PMID: 2824653
  4. Method to determine the reading frame of a protein from the purine/pyrimidine genome sequence and its possible evolutionary justification.
    Proc Natl Acad Sci U S A. 1981 Mar;78(3):1596-600 PMID: 6940175
  5. A function for human T200 in natural killer cytolysis.
    Transplantation. 1983 Aug;36(2):166-71 PMID: 6603688
  6. Structural features of Ly-5 glycoproteins of the mouse and counterparts in other mammals.
    Immunogenetics. 1988;28(4):271-7 PMID: 3417340
  7. Blocking of cytotoxic T cell function by monoclonal antibodies against the CD45 antigen (T200/leukocyte-common antigen).
    Transplantation. 1987 Dec;44(6):818-23 PMID: 2962345
  8. The leukocyte common antigen (CD45): a putative receptor-linked protein tyrosine phosphatase.
    Proc Natl Acad Sci U S A. 1988 Oct;85(19):7182-6 PMID: 2845400
  9. The predominant heavily glycosylated glycoproteins at the surface of rat lymphoid cells are differentiation antigens.
    Eur J Immunol. 1978 Dec;8(12):832-9 PMID: 310389
  10. Recognition of protein coding regions in DNA sequences.
    Nucleic Acids Res. 1982 Sep 11;10(17):5303-18 PMID: 7145702
  11. Lymphocyte cell surface glycoproteins which bind to soybean and peanut lectins.
    Immunology. 1982 Aug;46(4):713-26 PMID: 6980823
  12. A second signal for T cell mitogenesis provided by monoclonal antibodies CD45 (T200).
    Eur J Immunol. 1987 Oct;17(10):1447-51 PMID: 2960536
  13. MRC OX-22, a monoclonal antibody that labels a new subset of T lymphocytes and reacts with the high molecular weight form of the leukocyte-common antigen.
    J Exp Med. 1983 Sep 1;158(3):795-810 PMID: 6224884
  14. Interspecies spleen-myeloma hybrid producing monoclonal antibodies against mouse lymphocyte surface glycoprotein, T200.
    J Exp Med. 1978 Jul 1;148(1):313-23 PMID: 78961
  15. The current status and portability of our sequence handling software.
    Nucleic Acids Res. 1986 Jan 10;14(1):217-31 PMID: 3511446
  16. Alternative use of 5' exons in the specification of Ly-5 isoforms distinguishing hematopoietic cell lineages.
    Proc Natl Acad Sci U S A. 1987 Aug;84(15):5364-8 PMID: 3037546
  17. Monoclonal antibody to a human leukocyte-specific membrane glycoprotein probably homologous to the leukocyte-common (L-C) antigen of the rat.
    Eur J Immunol. 1980 Oct;10(10):737-44 PMID: 7428806
  18. Lymphocyte specific heterogeneity in the rat leucocyte common antigen (T200) is due to differences in polypeptide sequences near the NH2-terminus.
    EMBO J. 1987 May;6(5):1259-64 PMID: 2440674
  19. Structural variants of human T200 glycoprotein (leukocyte-common antigen).
    EMBO J. 1987 May;6(5):1251-7 PMID: 2956090
  20. B220: a B cell-specific member of th T200 glycoprotein family.
    Nature. 1981 Feb 19;289(5799):681-3 PMID: 6970340
  21. Quantitative serological analysis of a rabbit anti-rat lymphocyte serum and preliminary biochemical characterisation of the major antigen recognised.
    Transplantation. 1977 Apr;23(4):349-59 PMID: 68560
  22. Measurements of the effects that coding for a protein has on a DNA sequence and their use for finding genes.
    Nucleic Acids Res. 1984 Jan 11;12(1 Pt 2):551-67 PMID: 6364041
  23. Demonstration that the leukocyte common antigen CD45 is a protein tyrosine phosphatase.
    Biochemistry. 1988 Nov 29;27(24):8695-701 PMID: 2853967
  24. Evidence from cDNA clones that the rat leukocyte-common antigen (T200) spans the lipid bilayer and contains a cytoplasmic domain of 80,000 Mr.
    Cell. 1985 May;41(1):83-93 PMID: 3158393
  25. Evidence for the occurrence of O-glycosidically linked oligosaccharides of poly-N-acetyllactosamine type on the human leucocyte common antigen.
    Biochem Biophys Res Commun. 1983 Jan 27;110(2):424-31 PMID: 6601483
  26. Codon preference and its use in identifying protein coding regions in long DNA sequences.
    Nucleic Acids Res. 1982 Jan 11;10(1):141-56 PMID: 7063399
  27. Identification with a monoclonal antibody of a predominantly B lymphocyte-specific determinant of the human leukocyte common antigen. Evidence for structural and possible functional diversity of the human leukocyte common molecule.
    J Exp Med. 1981 Apr 1;153(4):753-65 PMID: 6166706
  28. Visualisation by low-angle shadowing of the leucocyte-common antigen. A major cell surface glycoprotein of lymphocytes.
    EMBO J. 1985 Nov;4(11):2827-30 PMID: 2933249
  29. Functional heterogeneity of CD4+ T lymphocytes: two subpopulations with counteracting immunoregulatory functions identified with the monoclonal antibodies WR16 and WR19.
    Immunology. 1987 Jun;61(2):159-65 PMID: 2954898
  30. Alternative splicing: a ubiquitous mechanism for the generation of multiple protein isoforms from single genes.
    Annu Rev Biochem. 1987;56:467-95 PMID: 3304142
  31. Role of the 2H4 molecule in the activation of suppressor inducer function.
    Eur J Immunol. 1988 May;18(5):731-7 PMID: 2967762
  32. Sequences of Ly-5 cDNA: isoform-related diversity of Ly-5 mRNA.
    Proc Natl Acad Sci U S A. 1986 Sep;83(18):6940-4 PMID: 2944116
  33. Differences in the surface proteins of mouse B and T cells.
    Proc Natl Acad Sci U S A. 1975 Jan;72(1):157-61 PMID: 47173
  34. Identification of lymphocyte integral membrane proteins as substrates for protein kinase C. Phosphorylation of the interleukin-2 receptor, class I HLA antigens, and T200 glycoprotein.
    J Biol Chem. 1986 Jun 25;261(18):8334-41 PMID: 2941417
  35. The monoclonal antibody, UCHL1, recognizes a 180,000 MW component of the human leucocyte-common antigen, CD45.
    Immunology. 1988 Jun;64(2):331-6 PMID: 2455685
  36. B-cell variant of mouse T200 (Ly-5): evidence for alternative mRNA splicing.
    Proc Natl Acad Sci U S A. 1987 Aug;84(15):5360-3 PMID: 2955416
  37. The isolation and characterization of the human suppressor inducer T cell subset.
    J Immunol. 1985 Mar;134(3):1508-15 PMID: 3155770
  38. Organization and expression of eucaryotic split genes coding for proteins.
    Annu Rev Biochem. 1981;50:349-83 PMID: 6791577
  39. Molecular and antigenic heterogeneity of the rat leukocyte-common antigen from thymocytes and T and B lymphocytes.
    Eur J Immunol. 1985 Feb;15(2):168-73 PMID: 2578966
Article Info
Journal
Immunogenetics
Abbr.
Immunogenetics
ISSN
0093-7711
DOI
10.1007/BF00352837
Published
1989-00-00
Pages
281-7
Language
English
Region
United States
NLM ID
0420404
Subset
IM
Databases
GENBANK
M18347, M18348, M18349
External Links
PubMed source DOI: 10.1007/BF00352837 View journal details
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