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PMID: 2524380 Published · ppublish English Journal Article Research Support, Non-U.S. Gov't

T cell tolerance to Mlsa encoded antigens in T cell receptor V beta 8.1 chain transgenic mice.

The EMBO journal ·Vol. 8 ·No. 3 ·1989-03-00 ·Pages 719-27

Pircher H, Mak TW, Lang R, Ballhausen W, Rüedi E, Hengartner H, Zinkernagel RM, Bürki K

Abstract

To study T cell tolerance, transgenic mice were generated that expressed the Mlsa-reactive T cell receptor (TCR) beta chain V beta 8.1 (cDNA) under the control of the H-2Kb promoter/immunoglobulin heavy chain enhancer on approximately 90% of peripheral T cells. In transgenic mice bearing Mlsa, thymocytes expressing the TCR at a high density were deleted and the percentage of Thy 1.2+ lymph node cells was reduced. The CD4/CD8 ratio of mature T cells was reversed in Mlsa and Mlsb transgenic mice independent of the H-2. RNA analysis and immunofluorescence with TCR V beta-specific antibodies revealed that expression of endogenous TCR beta genes was suppressed. Both Mlsa and Mlsb TCR beta chain transgenic mice mounted a T-cell-dependent IgG response against viral antigens, whereas the capacity to generate alloreactive and virus-specific cytotoxic T cells was impaired in TCR beta chain transgenic Mlsa, but not in transgenic Mlsb mice.

MeSH Terms
Animals Antigens, Differentiation, T-Lymphocyte Antigens, Surface/immunology CD8 Antigens Enhancer Elements, Genetic Gene Expression Regulation Genes, Immunoglobulin Immune Tolerance Mice Mice, Transgenic Minor Lymphocyte Stimulatory Antigens Receptors, Antigen, T-Cell/genetics,immunology Receptors, Antigen, T-Cell, alpha-beta T-Lymphocytes/classification,immunology
Chemicals
Antigens, Differentiation, T-Lymphocyte Antigens, Surface CD8 Antigens Minor Lymphocyte Stimulatory Antigens Receptors, Antigen, T-Cell Receptors, Antigen, T-Cell, alpha-beta
Authors & Affiliations
8 authors, click to expand affiliations / ORCID
Pircher H
Department of Experimental Pathology, University Hospital, Zürich, Switzerland.
Mak T W
Lang R
Ballhausen W
Rüedi E
Hengartner H
Zinkernagel R M
Bürki K
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Article Info
Journal
The EMBO journal
Abbr.
EMBO J
ISSN
0261-4189
Published
1989-03-00
Pages
719-27
Language
English
Region
England
NLM ID
8208664
PMCID
PMC400867
Subset
IM
External Links
PubMed source PMC Full Text View journal details
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