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PMID: 2530587 Published · ppublish English Comparative Study Journal Article Research Support, Non-U.S. Gov't

Cloning the interleukin 1 receptor from human T cells.

Proceedings of the National Academy of Sciences of the United States of America ·Vol. 86 ·No. 22 ·1989-11-00 ·Pages 8946-50

Sims JE, Acres RB, Grubin CE, McMahan CJ, Wignall JM, March CJ, Dower SK

Abstract

cDNA clones of the interleukin 1 (IL-1) receptor expressed in a human T-cell clone have been isolated by using a murine IL-1 receptor cDNA as a probe. The human and mouse receptors show a high degree of sequence conservation. Both are integral membrane proteins possessing a single membrane-spanning segment. Similar to the mouse receptor, the human IL-1 receptor contains a large cytoplasmic region and an extracellular, IL-1 binding portion composed of three immunoglobulin-like domains. When transfected into COS cells, the human IL-1 receptor cDNA clone leads to expression of two different affinity classes of receptors, with Ka values indistinguishable from those determined for IL-1 receptors in the original T-cell clone. An IL-1 receptor expressed in human dermal fibroblasts has also been cloned and sequenced and found to be identical to the IL-1 receptor expressed in T cells.

MeSH Terms
Amino Acid Sequence Animals B-Lymphocytes/immunology Base Sequence Cloning, Molecular DNA/genetics,isolation & purification Fibroblasts/immunology Gene Expression Genes Humans Interleukin-1/immunology,metabolism Kinetics Mice Molecular Sequence Data Receptors, Antigen, T-Cell/genetics Receptors, Immunologic/genetics,metabolism Receptors, Interleukin-1 Sequence Homology, Nucleic Acid T-Lymphocytes/immunology
Chemicals
Interleukin-1 Receptors, Antigen, T-Cell Receptors, Immunologic Receptors, Interleukin-1 DNA
Authors & Affiliations
7 authors, click to expand affiliations / ORCID
Sims J E
Immunex Corp., Seattle, WA 98101.
Acres R B
Grubin C E
McMahan C J
Wignall J M
March C J
Dower S K
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Article Info
Journal
Proceedings of the National Academy of Sciences of the United States of America
Abbr.
Proc Natl Acad Sci U S A
ISSN
0027-8424
DOI
10.1073/pnas.86.22.8946
Published
1989-11-00
Pages
8946-50
Language
English
Region
United States
NLM ID
7505876
PMCID
PMC298407
Subset
IM
Databases
GENBANK
M20658, M27492
External Links
PubMed source DOI: 10.1073/pnas.86.22.8946 PMC Full Text View journal details
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