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PMID: 2536111 Published · ppublish English Comparative Study Journal Article Research Support, Non-U.S. Gov't Research Support, U.S. Gov't, Non-P.H.S. Research Support, U.S. Gov't, P.H.S.

Nucleotide sequence of the human polyomavirus AS virus, an antigenic variant of BK virus.

Journal of virology ·Vol. 63 ·No. 2 ·1989-02-00 ·Pages 901-11

Tavis JE, Walker DL, Gardner SD, Frisque RJ

Abstract

The complete DNA sequence of the human polyomavirus AS virus (ASV) is presented. Although ASV can be differentiated antigenically from the other human polyomaviruses (BK and JC viruses), it shares 94.9% homology at the nucleotide level with the Dunlop strain of BK virus. Differences found in ASV relative to BK virus include the absence of tandem repeats in its regulatory region, the deletion of 32 nucleotides in the late mRNA leader region (altering the initiation codon for the agnoprotein), the presence of a cluster of base pair substitutions within the coding region of the major capsid protein, VP1, and the absence of 4 amino acids in the carboxy-terminal region of the early protein, T antigen. The 43 nucleotides deleted in the Dunlop strain of BK virus relative to the Gardner prototype strain of BK virus are present in ASV. Possible reasons for the distinct antigenicity of the ASV capsid, given the high degree of nucleotide homology with BK virus, are discussed. To reflect the high degree of sequence homology between ASV and BK virus, we suggest ASV be renamed BKV(AS).

MeSH Terms
Antigens, Viral/immunology BK Virus/genetics,immunology Base Sequence Genes, Viral Genetic Variation Molecular Sequence Data Polyomavirus/classification,genetics Sequence Homology, Nucleic Acid
Chemicals
Antigens, Viral
Authors & Affiliations
4 authors, click to expand affiliations / ORCID
Tavis J E
Department of Molecular and Cell Biology, Pennsylvania State University, University Park 16802.
Walker D L
Gardner S D
Frisque R J
References (54)
54 references, click to expand
  1. Bidirectional SV40 transcription mediated by tandem Sp1 binding interactions.
    Science. 1985 Nov 1;230(4725):511-7 PMID: 2996137
  2. Supercoil sequencing: a fast and simple method for sequencing plasmid DNA.
    DNA. 1985 Apr;4(2):165-70 PMID: 3996185
  3. Role of the agnoprotein in regulation of simian virus 40 replication and maturation pathways.
    J Virol. 1987 Mar;61(3):937-9 PMID: 3027418
  4. Complete nucleotide sequence of SV40 DNA.
    Nature. 1978 May 11;273(5658):113-20 PMID: 205802
  5. A domain of SV40 capsid polypeptide VP1 that specifies migration into the cell nucleus.
    EMBO J. 1986 Oct;5(10):2569-76 PMID: 3023047
  6. Molecular and biological properties of BK virus-IR, a BK virus variant isolated from a human tumor.
    J Virol. 1986 Aug;59(2):500-5 PMID: 3016311
  7. Selective extraction of polyoma DNA from infected mouse cell cultures.
    J Mol Biol. 1967 Jun 14;26(2):365-9 PMID: 4291934
  8. New human papovavirus (B.K.) isolated from urine after renal transplantation.
    Lancet. 1971 Jun 19;1(7712):1253-7 PMID: 4104714
  9. Naturally occurring BK virus variants (JL and Dik) with deletions in the putative early enhancer-promoter sequences.
    J Virol. 1985 Jan;53(1):302-5 PMID: 2981352
  10. Human polyomavirus JC virus genome.
    J Virol. 1984 Aug;51(2):458-69 PMID: 6086957
  11. A simple method for displaying the hydropathic character of a protein.
    J Mol Biol. 1982 May 5;157(1):105-32 PMID: 7108955
  12. Hybrid genomes of the polyomaviruses JC virus, BK virus, and simian virus 40: identification of sequences important for efficient transformation.
    J Virol. 1989 Feb;63(2):863-72 PMID: 2536108
  13. Mutations near the carboxyl terminus of the simian virus 40 large tumor antigen alter viral host range.
    J Virol. 1985 May;54(2):569-75 PMID: 2985819
  14. The genome of human papovavirus BKV.
    Cell. 1979 Dec;18(4):963-77 PMID: 229976
  15. BK virus DNA: complete nucleotide sequence of a human tumor virus.
    Science. 1979 Oct 26;206(4417):456-62 PMID: 228391
  16. Normal rat cell lines deficient in nuclear thymidine kinase.
    Virology. 1981 Aug;113(1):408-11 PMID: 7269249
  17. Enhancement of the transforming capacity of BK virus by partial deletion of the 68-base-pair tandem repeats.
    J Virol. 1985 Sep;55(3):867-9 PMID: 2991606
  18. Application of a microtechnique to viral serological investigations.
    J Immunol. 1962 Mar;88:320-9 PMID: 13910995
  19. Biochemical transfer of single-copy eucaryotic genes using total cellular DNA as donor.
    Cell. 1978 Jul;14(3):725-31 PMID: 210957
  20. Bifunctional messenger RNAs in eukaryotes.
    Cell. 1986 Nov 21;47(4):481-3 PMID: 3779834
  21. Point mutations define a sequence flanking the AUG initiator codon that modulates translation by eukaryotic ribosomes.
    Cell. 1986 Jan 31;44(2):283-92 PMID: 3943125
  22. Prevalence in England of antibody to human polyomavirus (B.k.).
    Br Med J. 1973 Jan 13;1(5845):77-8 PMID: 20791873
  23. Segments of simian virus 40 DNA spanning most of the leader sequence of the major late viral messenger RNA are dispensable.
    Proc Natl Acad Sci U S A. 1979 Jun;76(6):2556-60 PMID: 223148
  24. Efficient infection of monkey cells with DNA of simian virus 40.
    Proc Natl Acad Sci U S A. 1981 Dec;78(12):7575-8 PMID: 6278479
  25. The intranuclear location of simian virus 40 polypeptides VP2 and VP3 depends on a specific amino acid sequence.
    J Virol. 1987 Dec;61(12):3862-9 PMID: 2824820
  26. Viable deletion mutants of simian virus 40: selective isolation by means of a restriction endonuclease from Hemophilus parainfluenzae.
    Proc Natl Acad Sci U S A. 1974 Dec;71(12):4879-83 PMID: 4373732
  27. Simian virus 40 agnoprotein facilitates perinuclear-nuclear localization of VP1, the major capsid protein.
    J Virol. 1986 Dec;60(3):1055-61 PMID: 3023658
  28. Cultivation of papova-like virus from human brain with progressive multifocal leucoencephalopathy.
    Lancet. 1971 Jun 19;1(7712):1257-60 PMID: 4104715
  29. Structure and function of the transcriptional control region of nonpassaged BK virus.
    J Virol. 1987 May;61(5):1747-50 PMID: 3033304
  30. Compilation and analysis of sequences upstream from the translational start site in eukaryotic mRNAs.
    Nucleic Acids Res. 1984 Jan 25;12(2):857-72 PMID: 6694911
  31. Unidirectional digestion with exonuclease III creates targeted breakpoints for DNA sequencing.
    Gene. 1984 Jun;28(3):351-9 PMID: 6235151
  32. Construction and characterization of hybrid polyomavirus genomes.
    J Virol. 1986 Dec;60(3):960-71 PMID: 3023684
  33. The spliced structure of BK virus mRNAs in lytically infected and transormed cells.
    Virology. 1979 Aug;97(1):112-21 PMID: 224572
  34. Self-assembly of purified polyomavirus capsid protein VP1.
    Cell. 1986 Sep 12;46(6):895-904 PMID: 3019556
  35. Simian virus 40 agnoprotein facilitates normal nuclear location of the major capsid polypeptide and cell-to-cell spread of virus.
    J Virol. 1986 Dec;60(3):1098-106 PMID: 3023661
  36. A rapid alkaline extraction procedure for screening recombinant plasmid DNA.
    Nucleic Acids Res. 1979 Nov 24;7(6):1513-23 PMID: 388356
  37. Multiple origins of the complementary defective genomes of RF and origin proximal sequences of GS, two human papovavirus isolates.
    Virology. 1983 Dec;131(2):426-36 PMID: 6689230
  38. A prospective study of human polyomavirus infection in pregnancy.
    J Infect Dis. 1980 Jul;142(1):1-8 PMID: 6249869
  39. Prevalence of antibodies in human sera against JC virus, an isolate from a case of progressive multifocal leukoencephalopathy.
    J Infect Dis. 1973 Apr;127(4):467-70 PMID: 4571704
  40. A multiplicity of CCAAT box-binding proteins.
    Cell. 1987 Sep 11;50(6):863-72 PMID: 3476205
  41. Carboxyl-terminal mutants of the large tumor antigen of simian virus 40: a role for the early protein late in the lytic cycle.
    Proc Natl Acad Sci U S A. 1988 Jan;85(2):354-8 PMID: 2829182
  42. Regulation of protein synthesis in virus-infected animal cells.
    Adv Virus Res. 1986;31:229-92 PMID: 3019107
  43. Biological properties of simian virus 40 host range mutants lacking the COOH-terminus of large T antigen.
    Virology. 1987 Nov;161(1):170-80 PMID: 2823459
  44. Analysis of the early regulatory region of the human papovavirus BK.
    Virology. 1987 May;158(1):181-93 PMID: 3033888
  45. Purified transcription factor AP-1 interacts with TPA-inducible enhancer elements.
    Cell. 1987 Jun 19;49(6):741-52 PMID: 3034433
  46. Binding of cellular proteins to the regulatory region of BK virus DNA.
    J Virol. 1988 Sep;62(9):3388-98 PMID: 2841492
  47. Sequence at the 3' end of globin mRNA shows homology with immunoglobulin light chain mRNA.
    Nature. 1974 Nov 29;252(5482):359-62 PMID: 4139664
  48. Sequencing end-labeled DNA with base-specific chemical cleavages.
    Methods Enzymol. 1980;65(1):499-560 PMID: 6246368
  49. Strain differences and some serological observations on several isolates of human polyomaviruses.
    Prog Clin Biol Res. 1983;105:119-32 PMID: 6304748
  50. Decreasing the number of 68-base-pair tandem repeats in the BK virus transcriptional control region reduces plaque size and enhances transforming capacity.
    J Virol. 1985 Sep;55(3):823-5 PMID: 2991597
  51. Suppression of a VP1 mutant of simian virus 40 by missense mutations in serine codons of the viral agnogene.
    J Virol. 1983 Nov;48(2):405-9 PMID: 6312098
  52. Missense mutations in the VP1 gene of simian virus 40 that compensate for defects caused by deletions in the viral agnogene.
    J Virol. 1987 Oct;61(10):3190-8 PMID: 3041040
  53. Simian virus 40 maturation in cells harboring mutants deleted in the agnogene.
    J Biol Chem. 1985 Jan 25;260(2):1127-32 PMID: 2981833
  54. JC virus, a human polyomavirus associated with progressive multifocal leukoencephalopathy: additional biological characteristics and antigenic relationships.
    Infect Immun. 1977 Feb;15(2):656-62 PMID: 191404
Article Info
Journal
Journal of virology
Abbr.
J Virol
ISSN
0022-538X
DOI
10.1128/JVI.63.2.901-911.1989
Published
1989-02-00
Pages
901-11
Language
English
Region
United States
NLM ID
0113724
PMCID
PMC247764
Subset
IM
Grants
NIAID NIH HHS · AI11217 · United States
NCI NIH HHS · CA-44970 · United States
Databases
GENBANK
M23122
External Links
PubMed source DOI: 10.1128/JVI.63.2.901-911.1989 PMC Full Text View journal details
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