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PMID: 2536122 Published · ppublish English Comparative Study Journal Article Research Support, Non-U.S. Gov't

Two families of sequences in the small RNA-encoding region of Epstein-Barr virus (EBV) correlate with EBV types A and B.

Journal of virology ·Vol. 63 ·No. 2 ·1989-02-00 ·Pages 983-6

Arrand JR, Young LS, Tugwood JD

Abstract

DNA sequence analysis was carried out on the 1-kilobase SacI-EcoRI region of the EcoRI J fragment of four strains of Epstein-Barr virus (EBV) (MABA, P3HR-1, FF41, and NPC-5), and the sequences were compared with the prototype sequence from strain B95-8. Ten single-base changes which grouped the strains into two families (1 and 2) were found. Restriction endonuclease polymorphisms predicted from the sequences were used to classify the EBV DNA from a further 26 EBV-positive cell lines into these two families. The EBNA-2 types (A or B) of the strains were found to correlate with the J region type; EBNA-2 type A DNA regularly contained J region sequence type 1, while EBNA-2 type B DNA generally carried J region sequence type 2. These data are consistent with the notion of there being two distinct families of EBV with discrete, conserved differences in DNA sequence.

MeSH Terms
Base Sequence DNA, Viral/genetics Genes, Viral Herpesvirus 4, Human/classification,genetics RNA, Viral/genetics Restriction Mapping Sequence Homology, Nucleic Acid
Chemicals
DNA, Viral RNA, Viral
Authors & Affiliations
3 authors, click to expand affiliations / ORCID
Arrand J R
Paterson Institute for Cancer Research, Christie Hospital & Holt Radium Institute, Manchester, United Kingdom.
Young L S
Tugwood J D
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40 references, click to expand
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Article Info
Journal
Journal of virology
Abbr.
J Virol
ISSN
0022-538X
DOI
10.1128/JVI.63.2.983-986.1989
Published
1989-02-00
Pages
983-6
Language
English
Region
United States
NLM ID
0113724
PMCID
PMC247780
Subset
IM
External Links
PubMed source DOI: 10.1128/JVI.63.2.983-986.1989 PMC Full Text View journal details
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