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PMID: 6292473 Published · ppublish English Journal Article Research Support, Non-U.S. Gov't

Deletion of the nontransforming Epstein-Barr virus strain P3HR-1 causes fusion of the large internal repeat to the DSL region.

Journal of virology ·Vol. 43 ·No. 3 ·1982-09-00 ·Pages 952-68

Bornkamm GW, Hudewentz J, Freese UK, Zimber U

Abstract

The nontransforming Epstein-Barr virus (EBV) strain P3HR-1 is known to have a deletion of sequences of the long unique region adjacent to the large internal repeats. The deleted region is believed to be required for initiation of transformation. To establish a more detailed map of the deletion in P3HR-1 virus, SalI-A of the transforming strain M-ABA and of P3HR-1 virus was cloned into the cosmid vector pHC79 and multiplied in Escherichia coli. The cleavage sites for BamHI, BglII, EcoRI, PstI, SacI, SacII, and XhoI were determined in the recombinant plasmid clones. Analysis of the boundary between large internal repeats and the long unique region showed that in M-ABA (EBV) the transition is different from that in B95-8 virus. The map established for SalI-A of P3HR-1 virus revealed that, in contrast to previous reports, the deletion has a size of 6.5 kilobase pairs. It involves the junction between large internal repeats and the long unique region and includes more than half of the rightmost large internal repeat. The site of the deletion in the long unique region is located between a SacI and a SacII site, about 200 base pairs apart from each other. The sequences neighboring the deletion in the long unique region showed homology to the nonrepeated sequences of the DS(R) (duplicated sequence, right) region. Sequences of the large internal repeat are thus fused to sequences of the DS(L) (duplicated sequence, left) region in P3HR-1 virus DNA under elimination of the DS(L) repeats. Jijoye, the parental Burkitt lymphoma cell line from which the P3HR-1 line is derived by single-cell cloning, is known to produce a transforming virus. Analysis of the Jijoye (EBV) genome with cloned M-ABA (EBV) probes specific for the sequences missing in P3HR-1 virus revealed that the sequences of M-ABA (EBV) BamHI-H2 are not represented in Jijoye (EBV). In Jijoye (EBV) the complete DS(L) region including the DS(L) repeats is, however, conserved. Further analysis of Jijoye (EBV) and of Jijoye virustransformed cell lines will be helpful to narrow down the region required for transformation.

MeSH Terms
Cell Transformation, Viral Chromosome Deletion Cloning, Molecular DNA Restriction Enzymes Electrophoresis, Agar Gel Genes, Viral Genetic Linkage Herpesvirus 4, Human/genetics Humans Repetitive Sequences, Nucleic Acid
Chemicals
DNA Restriction Enzymes
Authors & Affiliations
4 authors, click to expand affiliations / ORCID
Bornkamm G W
Hudewentz J
Freese U K
Zimber U
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47 references, click to expand
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Article Info
Journal
Journal of virology
Abbr.
J Virol
ISSN
0022-538X
DOI
10.1128/JVI.43.3.952-968.1982
Published
1982-09-00
Pages
952-68
Language
English
Region
United States
NLM ID
0113724
PMCID
PMC256206
Subset
IM
External Links
PubMed source DOI: 10.1128/JVI.43.3.952-968.1982 PMC Full Text View journal details
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