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PMID: 2538835 Published · ppublish English Journal Article Research Support, Non-U.S. Gov't Research Support, U.S. Gov't, P.H.S.

Herpes simplex virus 1 helicase-primase: a complex of three herpes-encoded gene products.

Proceedings of the National Academy of Sciences of the United States of America ·Vol. 86 ·No. 7 ·1989-04-00 ·Pages 2186-9

Crute JJ, Tsurumi T, Zhu LA, Weller SK, Olivo PD, Challberg MD, Mocarski ES, Lehman IR

Abstract

In an earlier report, we described a DNA helicase that is specifically induced upon infection of Vero cells with herpes simplex virus 1. We have purified this enzyme to near homogeneity and found it to consist of three polypeptides with molecular weights of 120,000, 97,000, and 70,000. Immunochemical analysis has shown these polypeptides to be the products of three of the genes UL52, UL5, and UL8 that are required for replication of a plasmid containing a herpes simplex 1 origin (oriS). In addition to helicase activity, the enzyme contains a tightly associated DNA primase. Thus, the three-subunit enzyme is a helicase-primase complex that may prime lagging-strand synthesis as it unwinds DNA at the viral replication fork.

MeSH Terms
Adenosine Triphosphatases/genetics,isolation & purification Animals Chromatography, Gel Chromatography, Ion Exchange DNA Helicases/genetics,isolation & purification DNA Primase Genes Genes, Viral Multienzyme Complexes/genetics,isolation & purification Simplexvirus/enzymology,genetics Vero Cells Viral Proteins
Chemicals
Multienzyme Complexes Viral Proteins DNA Primase helicase-primase, Human herpesvirus 1 Adenosine Triphosphatases DNA Helicases
Authors & Affiliations
8 authors, click to expand affiliations / ORCID
Crute J J
Department of Biochemistry, Stanford University School of Medicine, CA 94305.
Tsurumi T
Zhu L A
Weller S K
Olivo P D
Challberg M D
Mocarski E S
Lehman I R
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Article Info
Journal
Proceedings of the National Academy of Sciences of the United States of America
Abbr.
Proc Natl Acad Sci U S A
ISSN
0027-8424
DOI
10.1073/pnas.86.7.2186
Published
1989-04-00
Pages
2186-9
Language
English
Region
United States
NLM ID
7505876
PMCID
PMC286876
Subset
IM
Grants
PHS HHS · A126538 · United States
NIAID NIH HHS · AI20211 · United States
NIGMS NIH HHS · GM06196 · United States
External Links
PubMed source DOI: 10.1073/pnas.86.7.2186 PMC Full Text View journal details
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