Home LiteratureArticle Details
PMID: 2542571 Published · ppublish English Journal Article Research Support, Non-U.S. Gov't

Induction of human interferon gene expression is associated with a nuclear factor that interacts with the NF-kappa B site of the human immunodeficiency virus enhancer.

Journal of virology ·Vol. 63 ·No. 6 ·1989-06-00 ·Pages 2557-66

Hiscott J, Alper D, Cohen L, Leblanc JF, Sportza L, Wong A, Xanthoudakis S

Abstract

The relationship between transcription of alpha and beta interferon (IFN-alpha and IFN-beta) genes and the interaction of IFN promoter-binding transcription factors has been examined in monoblastoid U937 cells following priming with recombinant IFN-alpha 2 (rIFN-alpha 2) and Sendai virus induction. Pretreatment of U937 cells with rIFN-alpha 2 prior to Sendai virus infection increased the mRNA levels of IFN-alpha 1, IFN-alpha 2, and IFN-beta as well as the final yield of biologically active IFN. Analysis of nuclear protein-IFN promoter DNA interactions by electrophoretic mobility-shift assays demonstrated increased factor binding to IFN-alpha 1 and IFN-beta regulatory domains, although no new induction-specific complexes were identified. On the basis of competition electrophoretic mobility-shift assay results, factors interacting with the IFN-alpha 1 and IFN-beta promoters appear to be distinct DNA-binding proteins. U937 factor binding was localized to the P2 domain (-64 to -55) of the IFN-beta regulatory element, a sequence motif with 80% homology to the recognition site of transcription factor NF-kappa B. Protein-DNA interactions within the IFN-beta P2 domain were, in fact, specifically competed by either excess homologous P2 fragment or the human immunodeficiency virus enhancer element which contains two duplicated NF-kappa B recognition sites. Hybrid promoter-chloramphenicol acetyltransferase fusion plasmids, containing either the IFN-beta regulatory element or the human immunodeficiency virus enhancer element linked to the simian virus 40 promoter, were analyzed for virus and phorbol ester inducibility in epithelial and lymphoid cells, respectively. In the 293 cell line, both plasmids were constitutively expressed but not virus inducible, while in Jurkat cells, chloramphenicol acetyltransferase activity from these plasmids was induced by tumor-promoting agent treatment. These experiments suggest that induction of IFN gene expression may be controlled in part by transcription regulatory proteins binding to an NF-kappa B-like site within the IFN-beta promoter.

MeSH Terms
Base Sequence Binding, Competitive Cell Line DNA, Viral/genetics Electrophoresis, Polyacrylamide Gel Enhancer Elements, Genetic Gene Expression Regulation HIV/genetics Humans Interferon Type I/genetics Molecular Sequence Data Parainfluenza Virus 1, Human/genetics Plasmids Promoter Regions, Genetic RNA, Messenger/analysis RNA, Viral/analysis Sequence Homology, Nucleic Acid Transcription Factors/genetics Transcription, Genetic Transfection
Chemicals
DNA, Viral Interferon Type I RNA, Messenger RNA, Viral Transcription Factors
Authors & Affiliations
7 authors, click to expand affiliations / ORCID
Hiscott J
Lady Davis Institute for Medical Research, Sir Mortimer B. Davis-Jewish General Hospital, Montreal, Quebec, Canada.
Alper D
Cohen L
Leblanc J F
Sportza L
Wong A
Xanthoudakis S
References (55)
55 references, click to expand
  1. Interferons and their actions.
    Annu Rev Biochem. 1987;56:727-77 PMID: 2441659
  2. trans activation of type 1 interferon promoters by simian virus 40 T antigen.
    Mol Cell Biol. 1988 Aug;8(8):3397-405 PMID: 2850492
  3. The effect of priming with interferon on interferon production by two lines of L cells.
    J Gen Virol. 1972 Apr;15(1):85-7 PMID: 4337220
  4. A new technique for the assay of infectivity of human adenovirus 5 DNA.
    Virology. 1973 Apr;52(2):456-67 PMID: 4705382
  5. Production of high-titered interferon in cultures of human diploid cells.
    Antimicrob Agents Chemother. 1972 Dec;2(6):476-84 PMID: 4670440
  6. Mapping of RNA by a modification of the Berk-Sharp procedure: the 5' termini of 15 S beta-globin mRNA precursor and mature 10 s beta-globin mRNA have identical map coordinates.
    Nucleic Acids Res. 1979 Nov 10;7(5):1175-93 PMID: 390497
  7. A gel electrophoresis method for quantifying the binding of proteins to specific DNA regions: application to components of the Escherichia coli lactose operon regulatory system.
    Nucleic Acids Res. 1981 Jul 10;9(13):3047-60 PMID: 6269071
  8. Reversible silencing of enhancers by sequences derived from the human IFN-alpha promoter.
    Cell. 1987 Sep 25;50(7):1057-69 PMID: 3040267
  9. Transient expression of the beta interferon promoter in human cells.
    Mol Cell Biol. 1987 Oct;7(10):3830-5 PMID: 2824999
  10. Purification of KBF1, a common factor binding to both H-2 and beta 2-microglobulin enhancers.
    EMBO J. 1987 Nov;6(11):3317-24 PMID: 3322806
  11. Two transcription factors, NF-kappa B and H2TF1, interact with a single regulatory sequence in the class I major histocompatibility complex promoter.
    Proc Natl Acad Sci U S A. 1988 Feb;85(3):723-7 PMID: 3422454
  12. Overlapping positive and negative regulatory domains of the human beta-interferon gene.
    Proc Natl Acad Sci U S A. 1988 Mar;85(5):1447-51 PMID: 3422743
  13. Regulation of gene expression by interferons: control of H-2 promoter responses.
    Science. 1988 Mar 11;239(4845):1302-6 PMID: 3125612
  14. Molecular cloning of an enhancer binding protein: isolation by screening of an expression library with a recognition site DNA.
    Cell. 1988 Feb 12;52(3):415-23 PMID: 2964277
  15. A high-efficiency HeLa cell nuclear transcription extract.
    DNA. 1988 Jan-Feb;7(1):47-55 PMID: 3349904
  16. Interferon response element of the human gene 6-16.
    EMBO J. 1988 Jan;7(1):85-92 PMID: 3359997
  17. Controlled transcription of a human alpha-interferon gene introduced into mouse L cells.
    Nature. 1982 May 13;297(5862):128-32 PMID: 6176871
  18. Inducibility of human beta-interferon gene in mouse L-cell clones.
    Nature. 1982 Jun 24;297(5868):650-4 PMID: 6178033
  19. Biochemistry of interferons and their actions.
    Annu Rev Biochem. 1982;51:251-82 PMID: 6180680
  20. Interferon production in human hematopoietic cell lines: response to chemicals and characterization of interferons.
    J Interferon Res. 1982;2(2):261-70 PMID: 6288815
  21. Recombinant genomes which express chloramphenicol acetyltransferase in mammalian cells.
    Mol Cell Biol. 1982 Sep;2(9):1044-51 PMID: 6960240
  22. Multiple point mutations affecting the simian virus 40 enhancer.
    Science. 1983 Feb 11;219(4585):626-31 PMID: 6297005
  23. Differential expression of human interferon genes.
    Nucleic Acids Res. 1984 May 11;12(9):3727-46 PMID: 6427755
  24. Delimitation and properties of DNA sequences required for the regulated expression of human interferon-beta gene.
    Cell. 1985 Jun;41(2):489-96 PMID: 2985279
  25. A 46-nucleotide promoter segment from an IFN-alpha gene renders an unrelated promoter inducible by virus.
    Cell. 1985 Jun;41(2):497-507 PMID: 3986909
  26. Human beta-interferon gene expression is regulated by an inducible enhancer element.
    Cell. 1985 Jun;41(2):509-20 PMID: 3986910
  27. alpha-Interferon-induced transcription of HLA and metallothionein genes containing homologous upstream sequences.
    Nature. 1985 Apr 18-24;314(6012):637-9 PMID: 3990797
  28. The human beta-interferon gene enhancer is under negative control.
    Cell. 1986 May 23;45(4):601-10 PMID: 3708688
  29. Detection of factors that interact with the human beta-interferon regulatory region in vivo by DNAase I footprinting.
    Cell. 1986 May 23;45(4):611-8 PMID: 3708689
  30. Multiple nuclear factors interact with the immunoglobulin enhancer sequences.
    Cell. 1986 Aug 29;46(5):705-16 PMID: 3091258
  31. Interferon response sequence potentiates activity of an enhancer in the promoter region of a mouse H-2 gene.
    Nature. 1986 Aug 21-27;322(6081):743-6 PMID: 3748155
  32. Activation of the human beta-interferon gene requires an interferon-inducible factor.
    Mol Cell Biol. 1986 Mar;6(3):801-10 PMID: 3773893
  33. Inducibility of kappa immunoglobulin enhancer-binding protein Nf-kappa B by a posttranslational mechanism.
    Cell. 1986 Dec 26;47(6):921-8 PMID: 3096580
  34. A transcriptional enhancer and an interferon-responsive sequence in major histocompatibility complex class I genes.
    Mol Cell Biol. 1986 Oct;6(10):3550-4 PMID: 3025599
  35. The interferon genes.
    Prog Nucleic Acid Res Mol Biol. 1986;33:251-300 PMID: 3025923
  36. An inducible transcription factor activates expression of human immunodeficiency virus in T cells.
    Nature. 1987 Apr 16-22;326(6114):711-3 PMID: 3031512
  37. Interferon-beta gene regulation: tandemly repeated sequences of a synthetic 6 bp oligomer function as a virus-inducible enhancer.
    Cell. 1987 May 8;49(3):357-67 PMID: 3032451
  38. Regulation of inducible and tissue-specific gene expression.
    Science. 1987 Jun 5;236(4806):1237-45 PMID: 3296191
  39. Identification of a nuclear DNA binding protein associated with the interferon-beta upstream regulatory region.
    J Biol Chem. 1987 Jun 15;262(17):8298-302 PMID: 3036808
  40. Single-step method of RNA isolation by acid guanidinium thiocyanate-phenol-chloroform extraction.
    Anal Biochem. 1987 Apr;162(1):156-9 PMID: 2440339
  41. The same inducible nuclear proteins regulates mitogen activation of both the interleukin-2 receptor-alpha gene and type 1 HIV.
    Cell. 1988 Jun 3;53(5):827-36 PMID: 2836068
  42. Interferon-induced nuclear factors that bind a shared promoter element correlate with positive and negative transcriptional control.
    Genes Dev. 1988 Apr;2(4):383-93 PMID: 3371658
  43. Regulation of interleukin 2 receptor alpha subunit (Tac or CD25 antigen) gene expression: binding of inducible nuclear proteins to discrete promoter sequences correlates with transcriptional activation.
    Proc Natl Acad Sci U S A. 1988 Jun;85(12):4468-72 PMID: 3132714
  44. Regulation of cytokine gene expression.
    Annu Rev Immunol. 1988;6:439-64 PMID: 3132954
  45. Identification and purification of a human immunoglobulin-enhancer-binding protein (NF-kappa B) that activates transcription from a human immunodeficiency virus type 1 promoter in vitro.
    Proc Natl Acad Sci U S A. 1988 Jul;85(13):4700-4 PMID: 3133660
  46. Interferon-induced binding of nuclear factors to promoter elements of the 2-5A synthetase gene.
    EMBO J. 1988 Mar;7(3):751-9 PMID: 2456211
  47. Regulated expression of a gene encoding a nuclear factor, IRF-1, that specifically binds to IFN-beta gene regulatory elements.
    Cell. 1988 Sep 9;54(6):903-13 PMID: 3409321
  48. Interferon-induced transcription of a major histocompatibility class I gene accompanies binding of inducible nuclear factors to the interferon consensus sequence.
    Proc Natl Acad Sci U S A. 1988 Aug;85(16):5884-8 PMID: 2457903
  49. Cooperativity and hierarchical levels of functional organization in the SV40 enhancer.
    Cell. 1988 Sep 23;54(7):943-53 PMID: 2843294
  50. I kappa B: a specific inhibitor of the NF-kappa B transcription factor.
    Science. 1988 Oct 28;242(4878):540-6 PMID: 3140380
  51. Regulation of HIV and HTLV gene expression.
    Genes Dev. 1988 Sep;2(9):1055-62 PMID: 2847958
  52. Evidence for a nuclear factor(s), IRF-1, mediating induction and silencing properties to human IFN-beta gene regulatory elements.
    EMBO J. 1988 Nov;7(11):3397-405 PMID: 2850164
  53. Multiple protein-DNA interactions within the human interferon-beta regulatory element.
    J Biol Chem. 1989 Jan 15;264(2):1139-45 PMID: 2910848
  54. Organization of the murine Mx gene and characterization of its interferon- and virus-inducible promoter.
    Mol Cell Biol. 1988 Aug;8(8):3065-79 PMID: 2974922
  55. Priming: a nonantiviral function of interferon.
    J Virol. 1971 Jun;7(6):792-801 PMID: 4327587
Article Info
Journal
Journal of virology
Abbr.
J Virol
ISSN
0022-538X
DOI
10.1128/JVI.63.6.2557-2566.1989
Published
1989-06-00
Pages
2557-66
Language
English
Region
United States
NLM ID
0113724
PMCID
PMC250725
Subset
IM
External Links
PubMed source DOI: 10.1128/JVI.63.6.2557-2566.1989 PMC Full Text View journal details
Analysis Services
Analysis Services

Contact

No. 2 Wenbo Road, Zhangqiu District, Jinan, Shandong

Qilu Normal University · Genelibs Bioinformatics Lab

750 Shunhua Rd, Jinan

2F, Bldg F, University Science Park

Tel: 0531-88819269

WeChat Official Account

Follow our WeChat subscription account for real-time updates and the latest in medical and biological research.


Business Email

E-mail: [email protected]

Copyright © 2026.Genelibs Jinan Honghui Biotechnology Co., Ltd. 鲁ICP备20007159号-2