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PMID: 2545906 Published · ppublish English Journal Article Research Support, U.S. Gov't, P.H.S.

Induction of anti-EBNA-1 protein by 12-O-tetradecanoylphorbol-13-acetate treatment of human lymphoblastoid cells.

Journal of virology ·Vol. 63 ·No. 8 ·1989-08-00 ·Pages 3315-22

Wen LT, Tanaka A, Nonoyama M

Abstract

Binding of the Epstein-Barr virus (EBV) nuclear antigen (EBNA-1) to BamHI-C DNA was studied by affinity column chromatography followed by immunoblotting with human serum specific for EBNA-1. Two species of EBNA-1 (68 and 70 kilodaltons) were identified in nuclear extracts of the EBV-positive Burkitt's lymphoma cell line Raji and not in nuclear extracts of the EBV-negative Burkitt's lymphoma cell line BJAB. Both EBNA-1s bound specifically to the region required for EBV plasmid DNA maintenance (oriP) located in the BamHI-C fragment. Upon treatment with 12-O-tetradecanoylphorbol-13-acetate, which activates latent EBV genome in Raji cells, the 68-kilodalton EBNA-1 was uncoupled from binding to EBV oriP. Nuclear extracts from 12-O-tetradecanoylphorbol-13-acetate-treated BJAB cells also uncoupled the binding of both EBNA-1s to oriP. DNA-cellulose column chromatography identified two protein species which competed for and uncoupled the binding of EBNA-1 to oriP. The two cellular competitors we called anti-EBNA-1 proteins had molecular masses of 60 and 40 kilodaltons, respectively. They were not found in nuclear extracts of BJAB cells not activated by 12-O-tetradecanoylphorbol-13-acetate.

MeSH Terms
Antigens, Viral Burkitt Lymphoma Cell Nucleus/immunology Chromatography, Affinity DNA, Viral/genetics,metabolism Epstein-Barr Virus Nuclear Antigens Genes, Viral Herpesvirus 4, Human/genetics,immunology Humans Immunoblotting Tetradecanoylphorbol Acetate/pharmacology Tumor Cells, Cultured Viral Proteins/biosynthesis,metabolism
Chemicals
Antigens, Viral DNA, Viral Epstein-Barr Virus Nuclear Antigens Viral Proteins Tetradecanoylphorbol Acetate
Authors & Affiliations
3 authors, click to expand affiliations / ORCID
Wen L T
Department of Virology, Showa University Research Institute for Biomedicine in Florida, St. Petersburg 33716.
Tanaka A
Nonoyama M
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Article Info
Journal
Journal of virology
Abbr.
J Virol
ISSN
0022-538X
DOI
10.1128/JVI.63.8.3315-3322.1989
Published
1989-08-00
Pages
3315-22
Language
English
Region
United States
NLM ID
0113724
PMCID
PMC250904
Subset
IM
Grants
NCI NIH HHS · R01 CA-31949 · United States
NCI NIH HHS · R01 CA-31950 · United States
NCI NIH HHS · R01 CA-36895 · United States
External Links
PubMed source DOI: 10.1128/JVI.63.8.3315-3322.1989 PMC Full Text View journal details
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