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PMID: 6328526 Published · ppublish English Journal Article Research Support, Non-U.S. Gov't Research Support, U.S. Gov't, P.H.S.

A cis-acting element from the Epstein-Barr viral genome that permits stable replication of recombinant plasmids in latently infected cells.

Proceedings of the National Academy of Sciences of the United States of America ·Vol. 81 ·No. 12 ·1984-06-00 ·Pages 3806-10

Yates J, Warren N, Reisman D, Sugden B

Abstract

The Epstein-Barr viral (EBV) genome of approximately equal to 170 kilobase pairs (kbp) is maintained as a plasmid in human B lymphoblasts transformed by the virus. We have identified a cis-acting element within 1.8 kbp of the viral genome that allows recombinant plasmids carrying it to be selected at high frequency and maintained as plasmids in cells latently infected by EBV. This functional element(s) requires a segment of DNA at least 800 bp and at most 1800 bp long, which contains a family of 30-bp tandem repeats at one end. Since this region confers efficient stable replication only to plasmids transfected into cells containing EBV genomes, its function probably requires trans-acting products encoded elsewhere in the viral genome.

MeSH Terms
DNA Replication DNA, Viral/genetics Gene Expression Regulation Genes, Regulator Genes, Viral Herpesvirus 4, Human/genetics Plasmids
Chemicals
DNA, Viral
Authors & Affiliations
4 authors, click to expand affiliations / ORCID
Yates J
Warren N
Reisman D
Sugden B
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44 references, click to expand
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Article Info
Journal
Proceedings of the National Academy of Sciences of the United States of America
Abbr.
Proc Natl Acad Sci U S A
ISSN
0027-8424
DOI
10.1073/pnas.81.12.3806
Published
1984-06-00
Pages
3806-10
Language
English
Region
United States
NLM ID
7505876
PMCID
PMC345309
Subset
IM
Grants
NCI NIH HHS · CA-07175 · United States
NCI NIH HHS · CA-22443 · United States
NCI NIH HHS · T32CA09135 · United States
Databases
GENBANK
V01509, V01554, V01555
External Links
PubMed source DOI: 10.1073/pnas.81.12.3806 PMC Full Text View journal details
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