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PMID: 2545921 Published · ppublish English Journal Article Research Support, Non-U.S. Gov't Research Support, U.S. Gov't, P.H.S.

Herpes simplex virus type 1 immediate-early protein Vmw110 reactivates latent herpes simplex virus type 2 in an in vitro latency system.

Journal of virology ·Vol. 63 ·No. 8 ·1989-08-00 ·Pages 3513-5

Harris RA, Everett RD, Zhu XX, Silverstein S, Preston CM

Abstract

Reactivation of latent herpes simplex virus type 2 (HSV-2) by the immediate-early protein Vmw110 was studied by using an in vitro latency system. Adenovirus recombinants that express Vmw110 reactivated latent HSV-2. An HSV-1 mutant possessing a deletion in a carboxy-terminal region of Vmw110 reactivated latent HSV-2, whereas mutant FXE, which has a deletion in the second exon, did not. Therefore, Vmw110 alone is required to reactivate latent HSV-2 in vitro, and the region of Vmw110 defined by the deletion in FXE is important for this process.

MeSH Terms
Adenoviridae/genetics Base Sequence Blotting, Southern Cells, Cultured DNA, Viral/genetics Humans Immediate-Early Proteins Mutation Nucleic Acid Hybridization Recombinant Proteins/genetics,physiology Restriction Mapping Simplexvirus/physiology Ubiquitin-Protein Ligases Viral Proteins/genetics,physiology
Chemicals
DNA, Viral IE1 protein, Human herpesvirus 1 Immediate-Early Proteins Recombinant Proteins Viral Proteins Ubiquitin-Protein Ligases Vmw110 protein, Human herpesvirus 1
Authors & Affiliations
5 authors, click to expand affiliations / ORCID
Harris R A
Medical Research Council Virology Unit, Institute of Virology, Glasgow, Scotland.
Everett R D
Zhu X X
Silverstein S
Preston C M
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Article Info
Journal
Journal of virology
Abbr.
J Virol
ISSN
0022-538X
DOI
10.1128/JVI.63.8.3513-3515.1989
Published
1989-08-00
Pages
3513-5
Language
English
Region
United States
NLM ID
0113724
PMCID
PMC250929
Subset
IM
Grants
NIGMS NIH HHS · GM38125 · United States
External Links
PubMed source DOI: 10.1128/JVI.63.8.3513-3515.1989 PMC Full Text View journal details
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