Conservation of the secondary structure elements of the 5'-untranslated region of cardio- and aphthovirus RNAs.

Nucleic acids research ·Vol. 17 ·No. 14 ·1989-07-25 ·Pages 5701-11

Pilipenko EV, Blinov VM, Chernov BK, Dmitrieva TM, Agol VI

Abstract

An analysis of published nucleotide sequences of the 5'-untranslated region (5'-UTR) of 7 cardioviruses and 3 aphthoviruses has allowed us to derive a consensus secondary structure model that differs from that previously proposed for the 5'-UTR of entero- and rhinoviruses, though all these viruses belong to the same family, Picornaviridae. The theoretical model derived here was experimentally supported by investigating the accessibility of encephalomyocarditis virus RNA to modifications with dimethyl sulfate and its susceptibility to S1 and cobra venom nucleases. The possible involvement of the 5"-UTR secondary structure domains in the translational control is briefly discussed.

MeSH Terms

Animals Base Sequence Carcinoma, Krebs 2 Encephalomyocarditis virus/genetics Mice Models, Molecular Molecular Sequence Data Nucleic Acid Conformation Oligonucleotide Probes RNA, Viral/genetics,isolation & purification Species Specificity

Chemicals

Oligonucleotide Probes RNA, Viral

Authors & Affiliations

5 authors, click to expand affiliations / ORCID

Pilipenko E V

Institute of Poliomyelitis and Viral Encephalitides, USSR Academy of Medical Sciences, Moscow.

Blinov V M

Chernov B K

Dmitrieva T M

Agol V I

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23 references, click to expand

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Article Info

Journal

Nucleic acids research

Abbr.

Nucleic Acids Res

ISSN

0305-1048

DOI

10.1093/nar/17.14.5701

Published

1989-07-25

Pages

5701-11

Language

English

Region

England

NLM ID

0411011

PMCID

PMC318190

Subset

External Links

PubMed source DOI: 10.1093/nar/17.14.5701 PMC Full Text View journal details

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Conservation of the secondary structure elements of the 5'-untranslated region of cardio- and aphthovirus RNAs.

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